IF4E1_PHAVU
ID IF4E1_PHAVU Reviewed; 230 AA.
AC V7BSD0; D3TI49; D3TI54; G8XRX6; G8XRX7; M4Y728; M4Y734; M4Y7P1; M4YAW3;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 19-FEB-2014, sequence version 1.
DT 03-AUG-2022, entry version 32.
DE RecName: Full=Eukaryotic translation initiation factor 4E-1 {ECO:0000303|PubMed:20447274, ECO:0000303|PubMed:23933781};
DE Short=PveIF4E {ECO:0000303|PubMed:20447274, ECO:0000303|PubMed:23933781};
DE Short=eIF-4E-1 {ECO:0000303|PubMed:20447274, ECO:0000303|PubMed:23933781};
DE AltName: Full=eIF-4F 25 kDa subunit {ECO:0000305};
DE AltName: Full=eIF-4F p26 subunit {ECO:0000305};
DE AltName: Full=mRNA cap-binding protein {ECO:0000303|PubMed:20447274, ECO:0000303|PubMed:23933781};
GN Name=eIF4E {ECO:0000303|PubMed:20447274, ECO:0000303|PubMed:23933781};
GN ORFNames=PHAVU_006G168400g {ECO:0000312|EMBL:ESW19950.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, FUNCTION (MICROBIAL INFECTION),
RP VARIANTS THR-6; LYS-53; TYR-65; GLU-76; GLY-111 AND 229-ALA-ILE-230 DELINS
RP VAL-VAL, SUBUNIT (MICROBIAL INFECTION), AND POLYMORPHISM.
RC STRAIN=cv. Amanda, cv. B/R RIL 105-25, cv. Baby Bop, cv. Black Knight,
RC cv. Clipper, cv. CY-10 S4, cv. Evolutie, cv. GN 1140, cv. Hystyle,
RC cv. Imuna, cv. IVT 7214, cv. Jolanda, cv. Laureat, cv. Midnight,
RC cv. Paloma, cv. Polder, cv. Sonesta, cv. USWK-6, and cv. USWKH x H S4;
RX PubMed=23933781; DOI=10.1007/s00122-013-2176-8;
RA Hart J.P., Griffiths P.D.;
RT "A series of eIF4E alleles at the Bc-3 locus are associated with recessive
RT resistance to Clover yellow vein virus in common bean.";
RL Theor. Appl. Genet. 126:2849-2863(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833;
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 8-222, FUNCTION, FUNCTION (MICROBIAL
RP INFECTION), VARIANTS LYS-53; TYR-65; GLU-76 AND GLY-111, SUBUNIT (MICROBIAL
RP INFECTION), AND POLYMORPHISM.
RC STRAIN=cv. Dubbele Witte, cv. I9365-31, cv. IVT7214, cv. Othello,
RC cv. Raven, cv. Sanilac, cv. Stringless Green Refugee, cv. Topcrop,
RC cv. USCR7, cv. USCR8, cv. USLK1, and cv. Widusa;
RX PubMed=20447274; DOI=10.1111/j.1364-3703.2009.00602.x;
RA Naderpour M., Lund O.S., Larsen R., Johansen E.;
RT "Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying
RT bc-3 is associated with the homozygotic presence of a mutated eIF4E
RT allele.";
RL Mol. Plant Pathol. 11:255-263(2010).
RN [4]
RP GENE FAMILY, AND REVIEW.
RX PubMed=24309680; DOI=10.1016/j.meegid.2013.11.024;
RA Moury B., Charron C., Janzac B., Simon V., Gallois J.L., Palloix A.,
RA Caranta C.;
RT "Evolution of plant eukaryotic initiation factor 4E (eIF4E) and potyvirus
RT genome-linked protein (VPg): a game of mirrors impacting resistance
RT spectrum and durability.";
RL Infect. Genet. Evol. 27:472-480(2014).
CC -!- FUNCTION: Component of the protein complex eIF4F, which is involved in
CC the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal
CC secondary structure and recruitment of mRNA to the ribosome (By
CC similarity). Recognizes and binds the 7-methylguanosine-containing mRNA
CC cap during an early step in the initiation of protein synthesis and
CC facilitates ribosome binding by inducing the unwinding of the mRNAs
CC secondary structures (By similarity). Key component of recessive
CC resistance to potyviruses (PubMed:20447274, PubMed:23933781).
CC {ECO:0000250|UniProtKB:P29557, ECO:0000269|PubMed:20447274,
CC ECO:0000269|PubMed:23933781}.
CC -!- FUNCTION: (Microbial infection) Susceptibility host factor required for
CC viral infection by recruiting viral RNAs to the host ribosomal complex
CC via an interaction with viral genome-linked protein (VPg).
CC {ECO:0000269|PubMed:20447274, ECO:0000269|PubMed:23933781}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions (By
CC similarity). It is composed of at least EIF4A, EIF4E and EIF4G (By
CC similarity). EIF4E is also known to interact with other partners (By
CC similarity). In higher plants two isoforms of EIF4F have been
CC identified, named isoform EIF4F and isoform EIF(iso)4F (By similarity).
CC Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has
CC subunits p82 and p28 (By similarity). {ECO:0000250|UniProtKB:P29557}.
CC -!- SUBUNIT: (Microbial infection) Interacts with potyvirus viral genome-
CC linked protein (VPg); this interaction is possible in susceptible hosts
CC but impaired in resistant plants. {ECO:0000305|PubMed:20447274,
CC ECO:0000305|PubMed:23933781}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C6ZJZ3}. Cytoplasm
CC {ECO:0000250|UniProtKB:C6ZJZ3}.
CC -!- PTM: According to the redox status, the Cys-128-Cys-166 disulfide
CC bridge may have a role in regulating protein function by affecting its
CC ability to bind capped mRNA. {ECO:0000250|UniProtKB:P29557}.
CC -!- POLYMORPHISM: Variant present in the strains cv. USLK1, cv. USCR8, cv.
CC USCR7, cv. Sonesta, cv. Polder, cv. Paloma, cv. Laureat and cv. Baby
CC Bop, allele PveIF4E(4), confers an increased resistance to clover
CC yellow vein virus (ClYVV) strain NY (ClYVV-NY) but susceptibility to
CC bean common mosaic necrosis virus (BCMNV) strain NL 3D (BCMNV-NL 3D).
CC {ECO:0000269|PubMed:20447274, ECO:0000269|PubMed:23933781}.
CC -!- POLYMORPHISM: Variant present in the strains cv. GN 1140, cv. Black
CC Knight, cv. Amanda, cv. Imuna, cv. Clipper and cv. CY-10 S4, alleles
CC PveIF4E(3) and cyv, confers an increased resistance to clover yellow
CC vein virus (ClYVV) strain NY (ClYVV-NY) but susceptibility to bean
CC common mosaic necrosis virus (BCMNV) strain NL 3D (BCMNV-NL 3D).
CC {ECO:0000269|PubMed:23933781}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Jolanda, cv. Imuna and
CC cv. Evolutie, allele desc, confers an increased resistance to clover
CC yellow vein virus (ClYVV) strain NY (ClYVV-NY) but susceptibility to
CC bean common mosaic necrosis virus (BCMNV) strain NL 3D (BCMNV-NL 3D).
CC {ECO:0000269|PubMed:23933781}.
CC -!- POLYMORPHISM: Variant present in the strains cv. IVT 7214, cv. B/R RIL
CC 105-25, cv. USWK-6 and cv. USWKH x H S4, allele bc-3, confers an
CC increased resistance to clover yellow vein virus (ClYVV) strain NY
CC (ClYVV-NY) and to bean common mosaic necrosis virus (BCMNV) strain NL
CC 3D (BCMNV-NL 3D). {ECO:0000269|PubMed:23933781}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Raven, allele
CC PveIF4E(2)Raven, allele PveIF4E(2), confers an increased resistance to
CC clover yellow vein virus (ClYVV) strain NY (ClYVV-NY) and to bean
CC common mosaic necrosis virus (BCMNV) strain NL 3D (BCMNV-NL 3D).
CC {ECO:0000269|PubMed:23933781}.
CC -!- MISCELLANEOUS: Displayed sequence is allele PveIF4E(1), sensitive to
CC potyviruses such as clover yellow vein virus (ClYVV) and bean common
CC mosaic necrosis virus (BCMNV). {ECO:0000305|PubMed:20447274,
CC ECO:0000305|PubMed:23933781}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC {ECO:0000305}.
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DR EMBL; KC417354; AGI41289.1; -; mRNA.
DR EMBL; KC417355; AGI41290.1; -; mRNA.
DR EMBL; KC417356; AGI41291.1; -; mRNA.
DR EMBL; KC417357; AGI41292.1; -; mRNA.
DR EMBL; KC417358; AGI41293.1; -; mRNA.
DR EMBL; KC417359; AGI41294.1; -; mRNA.
DR EMBL; KC417360; AGI41295.1; -; mRNA.
DR EMBL; KC417361; AGI41296.1; -; mRNA.
DR EMBL; KC417362; AGI41297.1; -; mRNA.
DR EMBL; KC417363; AGI41298.1; -; mRNA.
DR EMBL; KC417364; AGI41299.1; -; mRNA.
DR EMBL; KC417365; AGI41300.1; -; mRNA.
DR EMBL; KC417366; AGI41301.1; -; mRNA.
DR EMBL; KC417367; AGI41302.1; -; mRNA.
DR EMBL; KC417368; AGI41303.1; -; mRNA.
DR EMBL; KC417369; AGI41304.1; -; mRNA.
DR EMBL; KC417370; AGI41305.1; -; mRNA.
DR EMBL; KC417371; AGI41306.1; -; mRNA.
DR EMBL; KC417372; AGI41307.1; -; mRNA.
DR EMBL; CM002293; ESW19950.1; -; Genomic_DNA.
DR EMBL; EF571267; ABU54808.1; -; mRNA.
DR EMBL; EF571268; ABU54809.1; -; mRNA.
DR EMBL; EF571269; ABU54810.1; -; mRNA.
DR EMBL; EF571270; ABU54811.1; -; mRNA.
DR EMBL; EF571271; ABU54812.1; -; mRNA.
DR EMBL; EF571272; ABU54813.1; -; mRNA.
DR EMBL; EF571273; ABU54814.1; -; mRNA.
DR EMBL; EF571274; ABU54815.1; -; mRNA.
DR EMBL; EF571275; ABU54816.1; -; mRNA.
DR EMBL; EF571276; ABU54817.1; -; mRNA.
DR EMBL; EF571277; ABU54818.1; -; mRNA.
DR EMBL; FJ389546; ACQ59192.1; -; mRNA.
DR EMBL; FJ389547; ACQ59193.1; -; mRNA.
DR RefSeq; XP_007147956.1; XM_007147894.1.
DR STRING; 3885.XP_007147956.1; -.
DR EnsemblPlants; ESW19950; ESW19950; PHAVU_006G168400g.
DR GeneID; 18629048; -.
DR Gramene; ESW19950; ESW19950; PHAVU_006G168400g.
DR KEGG; pvu:PHAVU_006G168400g; -.
DR eggNOG; KOG1670; Eukaryota.
DR OMA; VKPRICL; -.
DR OrthoDB; 1394271at2759; -.
DR Proteomes; UP000000226; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; ISS:UniProtKB.
DR GO; GO:0006413; P:translational initiation; ISS:UniProtKB.
DR Gene3D; 3.30.760.10; -; 1.
DR InterPro; IPR023398; TIF_eIF4e-like.
DR InterPro; IPR001040; TIF_eIF_4E.
DR InterPro; IPR019770; TIF_eIF_4E_CS.
DR PANTHER; PTHR11960; PTHR11960; 1.
DR Pfam; PF01652; IF4E; 1.
DR SUPFAM; SSF55418; SSF55418; 1.
DR PROSITE; PS00813; IF4E; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Host-virus interaction; Initiation factor;
KW Nucleus; Plant defense; Protein biosynthesis; Reference proteome;
KW RNA-binding; Translation regulation.
FT CHAIN 1..230
FT /note="Eukaryotic translation initiation factor 4E-1"
FT /id="PRO_0000454066"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 55..58
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 65..101
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 149..158
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..36
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 73..78
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 105
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 123..124
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 173..178
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 218..222
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT DISULFID 128..166
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT VARIANT 6
FT /note="S -> T (in strain: Sonesta, Polder, Paloma, Laureat,
FT Baby Bop, Hystyle and Midnight. In strain: GN 1140, Black
FT Knight, Amanda, Imuna, Clipper and CY-10 S4, alleles
FT PveIF4E(3) and cyv. In strain: Jolanda, Imuna and Evolutie,
FT allele desc. In strain: IVT 7214, B/R RIL 105-25, USWK-6
FT and USWKH x H S4, allele bc-3)"
FT /evidence="ECO:0000269|PubMed:23933781"
FT VARIANT 53
FT /note="N -> K (in strain: USLK1, USCR8 and USCR7. In
FT strain: Imuna, Clipper and CY-10 S4, alleles PveIF4E(3) and
FT cyv. In strain: Jolanda, Imuna and Evolutie, allele desc.
FT In strain: Raven, allele PveIF4E(2). In strain: IVT 7214,
FT B/R RIL 105-25, USWK-6 and USWKH x H S4, allele bc-3)"
FT /evidence="ECO:0000269|PubMed:20447274,
FT ECO:0000269|PubMed:23933781"
FT VARIANT 65
FT /note="F -> Y (in strain: USLK1, USCR8 and USCR7. In
FT strain: Imuna, Clipper and CY-10 S4, alleles PveIF4E(3) and
FT cyv. In strain: Imuna and Evolutie, allele desc. In strain:
FT Raven, allele PveIF4E(2). In strain: IVT 7214, B/R RIL 105-
FT 25, USWK-6 and USWKH x H S4, allele bc-3)"
FT /evidence="ECO:0000269|PubMed:20447274,
FT ECO:0000269|PubMed:23933781"
FT VARIANT 76
FT /note="A -> E (in strain: USLK1, USCR8, USCR7, Sonesta,
FT Polder, Paloma, Laureat and Baby Bop, allele PveIF4E(4). In
FT strain: GN 1140, Black Knight, Amanda, Imuna, Clipper and
FT CY-10 S4, alleles PveIF4E(3) and cyv. In strain: Jolanda,
FT Imuna and Evolutie, allele desc. In strain: Raven, allele
FT PveIF4E(2). In strain: IVT 7214, B/R RIL 105-25, USWK-6 and
FT USWKH x H S4, allele bc-3)"
FT /evidence="ECO:0000269|PubMed:20447274,
FT ECO:0000269|PubMed:23933781"
FT VARIANT 111
FT /note="D -> G (in strain: USLK1, USCR8 and USCR7. In
FT strain: IVT 7214, B/R RIL 105-25, USWK-6 and USWKH x H S4,
FT allele bc-3)"
FT /evidence="ECO:0000269|PubMed:20447274,
FT ECO:0000269|PubMed:23933781"
FT VARIANT 229..230
FT /note="AI -> VV (in strain: Sonesta, Polder, Paloma,
FT Laureat, Baby Bop, Hystyle and Midnight. In strain, GN
FT 1140, Black Knight, Amanda, Imuna, Clipper and CY-10 S4,
FT alleles PveIF4E(3) and cyv. In strain: Jolanda, Imuna and
FT Evolutie, allele desc. In strain: IVT 7214, B/R RIL 105-25,
FT USWK-6 and USWKH x H S4, allele bc-3)"
FT /evidence="ECO:0000269|PubMed:23933781"
SQ SEQUENCE 230 AA; 26231 MW; 4BB71FEC3C432388 CRC64;
MVVEDSQKST ITDEQNPSRV DNDDDDLEDG EILEDADDAA SAASKPPSAF LRNPHPLENS
WTFWFDNPSA KSKQAAWGSS IRPIYTFSTV EEFWSIYNNI HHPSKLGVGA DFHCFKHKIE
PKWEDPICAN GGKWTMTFQR GKSDTSWLYT LLAMIGEQFD YGDEICGAVV NVRNRQDKIS
IWTKNASNEA AQMSIGKQWK EFLDYNEPIG FIFHEDAKKH ERSAKNKYAI
