ID   IF4E4_CAEEL             Reviewed;         212 AA.
AC   Q22888;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Eukaryotic translation initiation factor 4E-4;
DE            Short=eIF-4E-4;
DE            Short=eIF4E-4;
DE   AltName: Full=eIF-4F 25 kDa subunit;
DE   AltName: Full=mRNA cap-binding protein;
GN   Name=ife-4; ORFNames=C05D9.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=Bristol N2;
RX   PubMed=10744754; DOI=10.1074/jbc.275.14.10590;
RA   Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M.,
RA   Aamodt E.J., Blumenthal T., Rhoads R.E.;
RT   "Functional characterization of five eIF4E isoforms in Caenorhabditis
RT   elegans.";
RL   J. Biol. Chem. 275:10590-10596(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [3]
RP   TISSUE SPECIFICITY.
RC   STRAIN=Bristol N2;
RX   PubMed=11641215; DOI=10.1242/dev.128.20.3899;
RA   Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E.,
RA   Strome S.;
RT   "An isoform of eIF4E is a component of germ granules and is required for
RT   spermatogenesis in C. elegans.";
RL   Development 128:3899-3912(2001).
RN   [4]
RP   FUNCTION.
RX   PubMed=12422237;
RA   Stachelska A., Wieczorek Z., Ruszczynska K., Stolarski R., Pietrzak M.,
RA   Lamphear B.J., Rhoads R.E., Darzynkiewicz E., Jankowska-Anyszka M.;
RT   "Interaction of three Caenorhabditis elegans isoforms of translation
RT   initiation factor eIF4E with mono- and trimethylated mRNA 5' cap
RT   analogues.";
RL   Acta Biochim. Pol. 49:671-682(2002).
CC   -!- FUNCTION: Recognizes and binds the 7-methylguanosine-containing mRNA
CC       cap during an early step in the initiation of protein synthesis and
CC       facilitates ribosome binding by inducing the unwinding of the mRNAs
CC       secondary structures. All 5 eIF4E proteins bind monomethyl cap
CC       structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures
CC       which result from trans-splicing. Translation of trimethyl cap
CC       structure mRNAs may be regulated by intracellular redox state;
CC       disulfide bonds change the width and depth of the cap-binding cavity
CC       determining selectivity to mRNA caps. {ECO:0000269|PubMed:10744754,
CC       ECO:0000269|PubMed:12422237}.
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions. It is
CC       composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to
CC       interact with other partners.
CC   -!- TISSUE SPECIFICITY: Enriched in somatic cells.
CC       {ECO:0000269|PubMed:11641215}.
CC   -!- MISCELLANEOUS: Completely dispensible; null mutations have no effect on
CC       worm.
CC   -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC       {ECO:0000305}.
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DR   EMBL; AF214651; AAF62414.1; -; mRNA.
DR   EMBL; FO080359; CCD63145.1; -; Genomic_DNA.
DR   PIR; T31058; T31058.
DR   RefSeq; NP_508210.1; NM_075809.3.
DR   AlphaFoldDB; Q22888; -.
DR   SMR; Q22888; -.
DR   BioGRID; 45417; 5.
DR   STRING; 6239.C05D9.5; -.
DR   EPD; Q22888; -.
DR   PaxDb; Q22888; -.
DR   PeptideAtlas; Q22888; -.
DR   EnsemblMetazoa; C05D9.5.1; C05D9.5.1; WBGene00002062.
DR   GeneID; 180464; -.
DR   KEGG; cel:CELE_C05D9.5; -.
DR   UCSC; C05D9.5; c. elegans.
DR   CTD; 180464; -.
DR   WormBase; C05D9.5; CE07931; WBGene00002062; ife-4.
DR   eggNOG; KOG1669; Eukaryota.
DR   GeneTree; ENSGT00940000174225; -.
DR   HOGENOM; CLU_043552_3_3_1; -.
DR   InParanoid; Q22888; -.
DR   OMA; ANCHGGK; -.
DR   OrthoDB; 1394271at2759; -.
DR   PhylomeDB; Q22888; -.
DR   Reactome; R-CEL-1169408; ISG15 antiviral mechanism.
DR   PRO; PR:Q22888; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00002062; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR   GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; IBA:GO_Central.
DR   GO; GO:0000340; F:RNA 7-methylguanosine cap binding; IDA:WormBase.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   Gene3D; 3.30.760.10; -; 1.
DR   InterPro; IPR023398; TIF_eIF4e-like.
DR   InterPro; IPR001040; TIF_eIF_4E.
DR   InterPro; IPR019770; TIF_eIF_4E_CS.
DR   PANTHER; PTHR11960; PTHR11960; 1.
DR   Pfam; PF01652; IF4E; 1.
DR   SUPFAM; SSF55418; SSF55418; 1.
DR   PROSITE; PS00813; IF4E; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Initiation factor; Protein biosynthesis;
KW   Reference proteome; RNA-binding; Translation regulation.
FT   CHAIN           1..212
FT                   /note="Eukaryotic translation initiation factor 4E-4"
FT                   /id="PRO_0000193646"
FT   DISULFID        143..147
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   212 AA;  24584 MW;  87BA3DFE9FE8A486 CRC64;
     MEAETSTQEV VKQVKNLPIL MEDAPVGSDD HQLQYSYTFS YFMRPTGKFD PEDYASYVQP
     VGIMKSVEQF WSIMVHFKRP TEMCDKADIH FFKTGVKPVW EDPANCKGGK WIIRLKKGLS
     TRIWENLLMA IIGEQFLVGD ELCGAVCSIR NQEDIISLWN RNADDTPVTN RIRETLRSVL
     QLPQNTVLEY KRHDDCLRDQ SSYRHTTKNI CK