位置:首页 > 蛋白库 > IF4E_RABIT
IF4E_RABIT
ID   IF4E_RABIT              Reviewed;         217 AA.
AC   P29338;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Eukaryotic translation initiation factor 4E;
DE            Short=eIF-4E;
DE            Short=eIF4E;
DE   AltName: Full=eIF-4F 25 kDa subunit;
DE   AltName: Full=mRNA cap-binding protein;
GN   Name=EIF4E;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=1475206; DOI=10.1093/nar/20.23.6415;
RA   Rychlik W., Rhoads R.E.;
RT   "Nucleotide sequence of rabbit eIF-4E cDNA.";
RL   Nucleic Acids Res. 20:6415-6415(1992).
RN   [2]
RP   INTERACTION WITH EIF4A.
RX   PubMed=6853548; DOI=10.1016/s0021-9258(20)81965-6;
RA   Grifo J.A., Tahara S.M., Morgan M.A., Shatkin A.J., Merrick W.C.;
RT   "New initiation factor activity required for globin mRNA translation.";
RL   J. Biol. Chem. 258:5804-5810(1983).
CC   -!- FUNCTION: Recognizes and binds the 7-methylguanosine-containing mRNA
CC       cap during an early step in the initiation of protein synthesis and
CC       facilitates ribosome binding by inducing the unwinding of the mRNAs
CC       secondary structures. In addition to its role in translation
CC       initiation, also acts as a regulator of translation and stability in
CC       the cytoplasm (By similarity). Component of the CYFIP1-EIF4E-FMR1
CC       complex which binds to the mRNA cap and mediates translational
CC       repression: in the complex, EIF4E mediates the binding to the mRNA cap.
CC       Component of a multiprotein complex that sequesters and represses
CC       translation of proneurogenic factors during neurogenesis (By
CC       similarity). In P-bodies, component of a complex that mediates the
CC       storage of translationally inactive mRNAs in the cytoplasm and prevents
CC       their degradation (By similarity). May play an important role in
CC       spermatogenesis through translational regulation of stage-specific
CC       mRNAs during germ cell development (By similarity).
CC       {ECO:0000250|UniProtKB:P06730, ECO:0000250|UniProtKB:P63073,
CC       ECO:0000250|UniProtKB:P63074}.
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions (By
CC       similarity). It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3
CC       (PubMed:6853548). EIF4E is also known to interact with other partners.
CC       Interacts with EIF4ENIF1/4E-T; promotes recruitment to P-bodies and
CC       import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and
CC       EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin
CC       stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1
CC       causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and
CC       consequent initiation of translation. Interacts mutually exclusive with
CC       EIF4A1 or EIF4A2 (By similarity). Interacts with NGDN and PIWIL2.
CC       Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and
CC       FMR1. Interacts directly with CYFIP1. Interacts with CLOCK (By
CC       similarity). Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and
CC       AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts
CC       with LARP1. Interacts with METTL3. Interacts with RBM24; this
CC       interaction prevents EIF4E from binding to p53/TP53 mRNA and inhibits
CC       the assembly of translation initiation complex. Interacts with DDX3X;
CC       interaction is direct and in an RNA-independent manner; this
CC       interaction enhances EIF4E cap-binding ability and is required for the
CC       repression of cap-dependent translation and the increase of IRES-
CC       mediated translation. DDX3X competes with EIF4G1 for interaction with
CC       EIF4E (By similarity). Interacts with BTG4 and CNOT7 (By similarity).
CC       {ECO:0000250|UniProtKB:P06730, ECO:0000250|UniProtKB:P63073,
CC       ECO:0000269|PubMed:6853548}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000250|UniProtKB:P06730}.
CC       Cytoplasm {ECO:0000250|UniProtKB:P06730}. Cytoplasm, Stress granule
CC       {ECO:0000250|UniProtKB:P06730}. Nucleus {ECO:0000250|UniProtKB:P06730}.
CC       Note=Interaction with EIF4ENIF1/4E-T is required for localization to
CC       processing bodies (P-bodies). Imported in the nucleus via interaction
CC       with EIF4ENIF1/4E-T via a piggy-back mechanism.
CC       {ECO:0000250|UniProtKB:P06730}.
CC   -!- PTM: Phosphorylation increases the ability of the protein to bind to
CC       mRNA caps and to form the eIF4F complex.
CC       {ECO:0000250|UniProtKB:P06730}.
CC   -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X61939; CAA43943.1; -; mRNA.
DR   PIR; S30248; B26411.
DR   RefSeq; NP_001095180.1; NM_001101710.1.
DR   AlphaFoldDB; P29338; -.
DR   BMRB; P29338; -.
DR   SMR; P29338; -.
DR   STRING; 9986.ENSOCUP00000024878; -.
DR   BindingDB; P29338; -.
DR   ChEMBL; CHEMBL5636; -.
DR   iPTMnet; P29338; -.
DR   GeneID; 100009338; -.
DR   KEGG; ocu:100009338; -.
DR   CTD; 1977; -.
DR   eggNOG; KOG1670; Eukaryota.
DR   InParanoid; P29338; -.
DR   OrthoDB; 1394271at2759; -.
DR   TreeFam; TF101526; -.
DR   PRO; PR:P29338; -.
DR   Proteomes; UP000001811; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:AgBase.
DR   GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; ISS:UniProtKB.
DR   GO; GO:0005845; C:mRNA cap binding complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000932; C:P-body; ISS:UniProtKB.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:AgBase.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; ISS:AgBase.
DR   GO; GO:0019899; F:enzyme binding; ISS:AgBase.
DR   GO; GO:0031370; F:eukaryotic initiation factor 4G binding; ISS:AgBase.
DR   GO; GO:0000340; F:RNA 7-methylguanosine cap binding; ISS:UniProtKB.
DR   GO; GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
DR   GO; GO:0006417; P:regulation of translation; ISS:UniProtKB.
DR   Gene3D; 3.30.760.10; -; 1.
DR   InterPro; IPR023398; TIF_eIF4e-like.
DR   InterPro; IPR001040; TIF_eIF_4E.
DR   InterPro; IPR019770; TIF_eIF_4E_CS.
DR   PANTHER; PTHR11960; PTHR11960; 1.
DR   Pfam; PF01652; IF4E; 1.
DR   SUPFAM; SSF55418; SSF55418; 1.
DR   PROSITE; PS00813; IF4E; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Initiation factor; Nucleus; Phosphoprotein;
KW   Protein biosynthesis; Reference proteome; RNA-binding;
KW   Translation regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   CHAIN           2..217
FT                   /note="Eukaryotic translation initiation factor 4E"
FT                   /id="PRO_0000193636"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          37..40
FT                   /note="EIF4EBP1/2/3 binding"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   REGION          73..77
FT                   /note="EIF4EBP1/2/3 binding"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   REGION          132..139
FT                   /note="EIF4EBP1/2/3 binding"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   BINDING         56..57
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT                   /ligand_part_id="ChEBI:CHEBI:74429"
FT                   /ligand_part_note="m7GTP residue in mRNA cap"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   BINDING         102..103
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT                   /ligand_part_id="ChEBI:CHEBI:74429"
FT                   /ligand_part_note="m7GTP residue in mRNA cap"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   BINDING         157..162
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT                   /ligand_part_id="ChEBI:CHEBI:74429"
FT                   /ligand_part_note="m7GTP residue in mRNA cap"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   BINDING         205..207
FT                   /ligand="mRNA"
FT                   /ligand_id="ChEBI:CHEBI:33699"
FT                   /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT                   /ligand_part_id="ChEBI:CHEBI:74429"
FT                   /ligand_part_note="m7GTP residue in mRNA cap"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   MOD_RES         22
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
FT   MOD_RES         209
FT                   /note="Phosphoserine; by PKC and MKNK2"
FT                   /evidence="ECO:0000250|UniProtKB:P06730"
SQ   SEQUENCE   217 AA;  25049 MW;  B5A6BE12F4147159 CRC64;
     MATVEPETTP TPNPPPAEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL
     RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ
     QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENRDAVTHIG
     RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024