IF4G1_ARATH
ID IF4G1_ARATH Reviewed; 780 AA.
AC Q93ZT6; F4KDF4; Q8H0T8; Q8H179; Q9FJM7;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Eukaryotic translation initiation factor isoform 4G-1;
DE Short=eIF(iso)4G-1;
GN Name=EIF(ISO)4G1; OrderedLocusNames=At5g57870; ORFNames=MTI20.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=15163703; DOI=10.1128/jvi.78.12.6102-6111.2004;
RA Yoshii M., Nishikiori M., Tomita K., Yoshioka N., Kozuka R., Naito S.,
RA Ishikawa M.;
RT "The Arabidopsis cucumovirus multiplication 1 and 2 loci encode translation
RT initiation factors 4E and 4G.";
RL J. Virol. 78:6102-6111(2004).
RN [5]
RP REVIEW, AND SUBUNIT.
RX PubMed=16343979; DOI=10.1016/j.tplants.2005.11.004;
RA Robaglia C., Caranta C.;
RT "Translation initiation factors: a weak link in plant RNA virus
RT infection.";
RL Trends Plant Sci. 11:40-45(2006).
RN [6]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=17316629; DOI=10.1016/j.febslet.2007.02.007;
RA Nicaise V., Gallois J.L., Chafiai F., Allen L.M., Schurdi-Levraud V.,
RA Browning K.S., Candresse T., Caranta C., Le Gall O., German-Retana S.;
RT "Coordinated and selective recruitment of eIF4E and eIF4G factors for
RT potyvirus infection in Arabidopsis thaliana.";
RL FEBS Lett. 581:1041-1046(2007).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=20694742; DOI=10.1007/s11103-010-9670-z;
RA Lellis A.D., Allen M.L., Aertker A.W., Tran J.K., Hillis D.M., Harbin C.R.,
RA Caldwell C., Gallie D.R., Browning K.S.;
RT "Deletion of the eIFiso4G subunit of the Arabidopsis eIFiso4F translation
RT initiation complex impairs health and viability.";
RL Plant Mol. Biol. 74:249-263(2010).
CC -!- FUNCTION: Plays a role in the accumulation of some potyvirus during
CC viral infection. {ECO:0000269|PubMed:17316629}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G. In higher plants two
CC isoforms of EIF4F have been identified, named isoform EIF4F and isoform
CC EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform
CC EIF(iso)4F has subunits p82 and p28. {ECO:0000269|PubMed:16343979}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93ZT6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93ZT6-2; Sequence=VSP_044532;
CC -!- DISRUPTION PHENOTYPE: Displays resistance to potyvirus (PPV or LMV)
CC infection. I4g1/i4g2 double mutants show reduced germination rates,
CC slow growth rates, moderate chlorosis, late flowering, impaired
CC fertility and reduced long term seed viability. They also exhibit
CC altered responses to dehydration, salinity, and heat stress. The i4g1
CC and i4g2 double mutant has reduced amounts of chlorophyll a and b.
CC {ECO:0000269|PubMed:17316629, ECO:0000269|PubMed:20694742}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4G family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08857.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB013396; BAB08857.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96964.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96965.1; -; Genomic_DNA.
DR EMBL; AY056266; AAL07115.1; -; mRNA.
DR EMBL; AY091258; AAM14197.1; -; mRNA.
DR EMBL; BT000467; AAN17444.1; -; mRNA.
DR EMBL; BT003424; AAO30087.1; -; mRNA.
DR EMBL; BT002056; AAN72067.1; -; mRNA.
DR RefSeq; NP_200595.2; NM_125172.2. [Q93ZT6-2]
DR RefSeq; NP_851207.1; NM_180876.2. [Q93ZT6-1]
DR AlphaFoldDB; Q93ZT6; -.
DR SMR; Q93ZT6; -.
DR BioGRID; 21141; 6.
DR IntAct; Q93ZT6; 4.
DR STRING; 3702.AT5G57870.1; -.
DR iPTMnet; Q93ZT6; -.
DR PaxDb; Q93ZT6; -.
DR PRIDE; Q93ZT6; -.
DR ProteomicsDB; 232186; -. [Q93ZT6-1]
DR EnsemblPlants; AT5G57870.1; AT5G57870.1; AT5G57870. [Q93ZT6-1]
DR EnsemblPlants; AT5G57870.2; AT5G57870.2; AT5G57870. [Q93ZT6-2]
DR GeneID; 835897; -.
DR Gramene; AT5G57870.1; AT5G57870.1; AT5G57870. [Q93ZT6-1]
DR Gramene; AT5G57870.2; AT5G57870.2; AT5G57870. [Q93ZT6-2]
DR KEGG; ath:AT5G57870; -.
DR Araport; AT5G57870; -.
DR TAIR; locus:2174353; AT5G57870.
DR eggNOG; KOG0401; Eukaryota.
DR InParanoid; Q93ZT6; -.
DR OMA; MEKDHQP; -.
DR OrthoDB; 594395at2759; -.
DR PhylomeDB; Q93ZT6; -.
DR PRO; PR:Q93ZT6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93ZT6; baseline and differential.
DR Genevisible; Q93ZT6; AT.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045208; IF4G.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR PANTHER; PTHR23253; PTHR23253; 1.
DR Pfam; PF02847; MA3; 1.
DR Pfam; PF02854; MIF4G; 1.
DR SMART; SM00544; MA3; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS51366; MI; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Initiation factor; Protein biosynthesis;
KW Reference proteome; Translation regulation.
FT CHAIN 1..780
FT /note="Eukaryotic translation initiation factor isoform 4G-
FT 1"
FT /id="PRO_0000420542"
FT DOMAIN 216..440
FT /note="MIF4G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 616..738
FT /note="MI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 529..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 166..189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 115..118
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_044532"
FT CONFLICT 546
FT /note="S -> T (in Ref. 3; AAN72067)"
FT /evidence="ECO:0000305"
FT CONFLICT 610
FT /note="P -> T (in Ref. 3; AAN17444/AAO30087)"
FT /evidence="ECO:0000305"
FT CONFLICT 690
FT /note="L -> I (in Ref. 3; AAN17444/AAO30087)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 780 AA; 85572 MW; 122772273F6C6234 CRC64;
MQQGDQTVLS LRPGGGRGNR LFGSSSSSSS LSFGSLSSSD LPLLRPHGGA PASSFPFKGG
DSRFDGRERV KYTREQLLEL KETTQLSDEI LRVQRETAAE LFGEEGTWAR GESVVSNLVP
VQSASRFSEP DSRDWRSRST QPPPSGEERS WDNLREAKDS RYVEASQYNR QDQPNSQFSR
ANISSNQGGG PAPVLVKAEV PWSARRGNLS ENDRVLKTVK GILNKLTPEK YDLLKGQLIE
SGITSADILK GVITLIFDKA VLEPTFCPMY AKLCSDINDQ LPTFPPAEPG DKEITFKRVL
LNICQEAFEG ASQLREELRQ MSAPDQEAER NDKEKLLKLK TLGNIRLIGE LLKQKMVPEK
IVHHIVQELL GADEKVCPAE ENVEAICHFF KTIGKQLDGN VKSKRINDVY FKRLQALSKN
PQLELRLRFM VQNIIDMRSN GWVPRREEMK ARTITEIHTE AEKNLGLRPG ATANMRRGMV
SSGGPVSPGP VYPGGRPGAG GLMPGMPGTR RMPGMPGVDN DNWEVPRTRS MSRRDGPGPL
HSPAVSKSAS MNTRLLPQGS SGIMSGKTSA LLQGSGSVSR PVTVSAERPA QSVAPLTVPV
PVEKPQPSGP KLSEEVLQRK TKSLLEEYFN VRLLGEALQC VEELGLPSYH PEFVKEAISL
SLEKSPPVVE PIATLLEYLL SKKVVAPKDL ETGFLLYGAM LDDIGIDLPK APNNFGEIVG
KLILAGGVDF KLVREIIGKM EDDRFQKMVV DAAVRIVESS EQGKSLLASQ AADIEACRNL
