IF4G2_ARATH
ID IF4G2_ARATH Reviewed; 747 AA.
AC O82233; Q8W4L4;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Eukaryotic translation initiation factor isoform 4G-2;
DE Short=eIF(iso)4G-2;
GN Name=EIF(ISO)4G2; OrderedLocusNames=At2g24050; ORFNames=T29E15.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=15163703; DOI=10.1128/jvi.78.12.6102-6111.2004;
RA Yoshii M., Nishikiori M., Tomita K., Yoshioka N., Kozuka R., Naito S.,
RA Ishikawa M.;
RT "The Arabidopsis cucumovirus multiplication 1 and 2 loci encode translation
RT initiation factors 4E and 4G.";
RL J. Virol. 78:6102-6111(2004).
RN [5]
RP REVIEW, AND SUBUNIT.
RX PubMed=16343979; DOI=10.1016/j.tplants.2005.11.004;
RA Robaglia C., Caranta C.;
RT "Translation initiation factors: a weak link in plant RNA virus
RT infection.";
RL Trends Plant Sci. 11:40-45(2006).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=17316629; DOI=10.1016/j.febslet.2007.02.007;
RA Nicaise V., Gallois J.L., Chafiai F., Allen L.M., Schurdi-Levraud V.,
RA Browning K.S., Candresse T., Caranta C., Le Gall O., German-Retana S.;
RT "Coordinated and selective recruitment of eIF4E and eIF4G factors for
RT potyvirus infection in Arabidopsis thaliana.";
RL FEBS Lett. 581:1041-1046(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=20694742; DOI=10.1007/s11103-010-9670-z;
RA Lellis A.D., Allen M.L., Aertker A.W., Tran J.K., Hillis D.M., Harbin C.R.,
RA Caldwell C., Gallie D.R., Browning K.S.;
RT "Deletion of the eIFiso4G subunit of the Arabidopsis eIFiso4F translation
RT initiation complex impairs health and viability.";
RL Plant Mol. Biol. 74:249-263(2010).
CC -!- FUNCTION: Plays a role in the accumulation of some potyvirus during
CC viral infection. {ECO:0000250}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G. In higher plants two
CC isoforms of EIF4F have been identified, named isoform EIF4F and isoform
CC EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform
CC EIF(iso)4F has subunits p82 and p28. {ECO:0000269|PubMed:16343979}.
CC -!- DISRUPTION PHENOTYPE: Displays no resistance to potyvirus. Possesses
CC reduced amounts of chlorophyll a and b. I4g1 and i4g2 double mutants
CC show reduced germination rates, slow growth rates, moderate chlorosis,
CC late flowering, impaired fertility and reduced long term seed
CC viability. They also exhibit altered responses to dehydration,
CC salinity, and heat stress. The i4g1 and i4g2 double mutant has reduced
CC amounts of chlorophyll a and b. {ECO:0000269|PubMed:17316629,
CC ECO:0000269|PubMed:20694742}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4G family.
CC {ECO:0000305}.
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DR EMBL; AC005170; AAC63673.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07523.1; -; Genomic_DNA.
DR EMBL; AY062494; AAL32572.1; -; mRNA.
DR EMBL; BT002087; AAN72098.1; -; mRNA.
DR PIR; A84632; A84632.
DR RefSeq; NP_179983.1; NM_127967.4.
DR AlphaFoldDB; O82233; -.
DR SMR; O82233; -.
DR BioGRID; 2291; 3.
DR IntAct; O82233; 3.
DR STRING; 3702.AT2G24050.1; -.
DR iPTMnet; O82233; -.
DR PaxDb; O82233; -.
DR PRIDE; O82233; -.
DR ProteomicsDB; 232202; -.
DR EnsemblPlants; AT2G24050.1; AT2G24050.1; AT2G24050.
DR GeneID; 816939; -.
DR Gramene; AT2G24050.1; AT2G24050.1; AT2G24050.
DR KEGG; ath:AT2G24050; -.
DR Araport; AT2G24050; -.
DR TAIR; locus:2061370; AT2G24050.
DR eggNOG; KOG0401; Eukaryota.
DR HOGENOM; CLU_021872_0_0_1; -.
DR InParanoid; O82233; -.
DR OMA; ENAILQP; -.
DR OrthoDB; 594395at2759; -.
DR PhylomeDB; O82233; -.
DR PRO; PR:O82233; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82233; baseline and differential.
DR Genevisible; O82233; AT.
DR GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045208; IF4G.
DR InterPro; IPR003891; Initiation_fac_eIF4g_MI.
DR InterPro; IPR003890; MIF4G-like_typ-3.
DR PANTHER; PTHR23253; PTHR23253; 1.
DR Pfam; PF02847; MA3; 1.
DR Pfam; PF02854; MIF4G; 1.
DR SMART; SM00544; MA3; 1.
DR SMART; SM00543; MIF4G; 1.
DR SUPFAM; SSF48371; SSF48371; 2.
DR PROSITE; PS51366; MI; 1.
PE 1: Evidence at protein level;
KW Initiation factor; Protein biosynthesis; Reference proteome;
KW Translation regulation.
FT CHAIN 1..747
FT /note="Eukaryotic translation initiation factor isoform 4G-
FT 2"
FT /id="PRO_0000420543"
FT DOMAIN 176..400
FT /note="MIF4G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT DOMAIN 582..704
FT /note="MI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00698"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 489..580
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 490..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 536..551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 576
FT /note="N -> D (in Ref. 3; AAL32572/AAN72098)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 747 AA; 82890 MW; C7AFC0731A8341FA CRC64;
MQQQGEPSVL SLRPGGGGGK SRLFVPRFSS SSSFDLTNGG SEETPFPVKR ENSGERVRFS
REEILQHRES VQVSDEILRR CKEIAVELFG EEQSWGNHAA ESKITNHTQH RHTETDNRDW
HSRSQIPTSG KEWLRDDPRE AKSTWQGSGP TPVLIKAEVP WSAKRGALSD KDRVVKSVKG
ILNKLTPEKY ELLKGQLIDA GITSADILKE VIQLIFENAI LQPTFCEMYA LLCFDINGQL
PSFPSEEPGG KEITFKRVLL NNCQEAFEGA GKLKEEIRQM TNPDQEMERM DKEKMAKLRT
LGNIRLIGEL LKQKMVPEKI VHHIVQELLG DDTKACPAEG DVEALCQFFI TIGKQLDDSP
RSRGINDTYF GRLKELARHP QLELRLRFMV QNVVDLRANK WVPRREEVKA KKINEIHSEA
ERNLGMRPGA MASMRNNNNN RAAVSGAADG MGLGNILGRP GTGGMMPGMP GTRVMPMDED
GWEMARTRSM PRGNRQTVQQ PRFQPPPAIN KSLSVNSRLL PQGSGGLLNG GGRPSPLLQG
NGSSSAPQAS KPIPTVEKPQ PRSQPQPQPQ AAPLANSLNA GELERKTKSL LEEYFSIRLV
DEALQCVEEL KSPSYHPELV KETISLGLEK NPPLVEPIAK LLKHLISKNV LTSKDLGAGC
LLYGSMLDDI GIDLPKAPNS FGEFLGELVS AKVLDFELVR DVLKKMEDEW FRKTVLNAVI
KSVRECPSGQ SVLDSQAVEV EACQSLL
