IF5A1_TOBAC
ID IF5A1_TOBAC Reviewed; 159 AA.
AC P69040; P24921;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Eukaryotic translation initiation factor 5A-1;
DE Short=eIF-5A-1;
DE AltName: Full=eIF-4D;
GN Name=EIF-5A1;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leaf;
RX PubMed=1542563; DOI=10.1093/nar/20.4.665;
RA Chamot D., Kuhlemeter C.;
RT "Differential expression of genes encoding the hypusine-containing
RT translation initiation factor, eIF-5A, in tobacco.";
RL Nucleic Acids Res. 20:665-669(1992).
CC -!- FUNCTION: The precise role of eIF-5A in protein biosynthesis is not
CC known but it functions by promoting the formation of the first peptide
CC bond.
CC -!- PTM: Lys-52 undergoes hypusination, a unique post-translational
CC modification that consists in the addition of a butylamino group from
CC spermidine to lysine side chain, leading to the formation of the
CC unusual amino acid hypusine. eIF-5As are the only known proteins to
CC undergo this modification, which is essential for their function.
CC {ECO:0000250|UniProtKB:Q9XI91}.
CC -!- MISCELLANEOUS: There are at least two genes for eIF-5A in tobacco: 5A1
CC may regulate the light-dependent translation of specific transcripts
CC while 5A2 may be a housekeeping protein.
CC -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
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DR EMBL; X63543; CAA45105.1; -; Genomic_DNA.
DR PIR; S21060; S21060.
DR RefSeq; XP_016502573.1; XM_016647087.1.
DR AlphaFoldDB; P69040; -.
DR SMR; P69040; -.
DR GeneID; 107820748; -.
DR KEGG; nta:107820748; -.
DR OMA; TIMINIQ; -.
DR OrthoDB; 1370513at2759; -.
DR PhylomeDB; P69040; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0045901; P:positive regulation of translational elongation; IBA:GO_Central.
DR GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR001884; IF5A-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR InterPro; IPR020189; Transl_elong_IF5A_C.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11673; PTHR11673; 1.
DR Pfam; PF01287; eIF-5a; 1.
DR PIRSF; PIRSF003025; eIF5A; 1.
DR SMART; SM01376; eIF-5a; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00037; eIF_5A; 1.
DR PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE 3: Inferred from homology;
KW Hypusine; Initiation factor; Protein biosynthesis; Reference proteome.
FT CHAIN 1..159
FT /note="Eukaryotic translation initiation factor 5A-1"
FT /id="PRO_0000142482"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 52
FT /note="Hypusine"
FT /evidence="ECO:0000250|UniProtKB:Q9XI91"
SQ SEQUENCE 159 AA; 17371 MW; 5174BC5809E8E275 CRC64;
MSDEEHHFES KADAGASKTY PQQAGTIRKN GHIVIKNRPC KVVEVSTSKT GKHGHAKCHF
VAIDIFTGKK LEDIVPSSHN CDVPHVNRTD YQLIDISEDG FVSLLTENGN TKDDLRLPTD
DNLLALIKDG FAEGKDLVLS VMSAMGEEQI CGIKDVGPK
