ID   IF5A3_SOLTU             Reviewed;         160 AA.
AC   P56335;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Eukaryotic translation initiation factor 5A-3;
DE            Short=eIF-5A-3;
DE   AltName: Full=eIF-4D;
GN   Name=EIF5A3;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Irish Cobbler;
RA   In J.G., Fujino K., Kikuta Y.;
RT   "Nucleotide sequence of five cDNAs encoding eukaryotic translation
RT   initiation factor 5A (eIF-5A) from potato.";
RL   (er) Plant Gene Register PGR97-147(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. DM1-3 516 R44;
RX   PubMed=21743474; DOI=10.1038/nature10158;
RG   The Potato Genome Sequencing Consortium;
RT   "Genome sequence and analysis of the tuber crop potato.";
RL   Nature 475:189-195(2011).
CC   -!- FUNCTION: The precise role of eIF-5A in protein biosynthesis is not
CC       known but it functions by promoting the formation of the first peptide
CC       bond.
CC   -!- PTM: Lys-52 undergoes hypusination, a unique post-translational
CC       modification that consists in the addition of a butylamino group from
CC       spermidine to lysine side chain, leading to the formation of the
CC       unusual amino acid hypusine. eIF-5As are the only known proteins to
CC       undergo this modification, which is essential for their function.
CC       {ECO:0000250|UniProtKB:Q9XI91}.
CC   -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
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DR   EMBL; AB004824; BAA20877.1; -; mRNA.
DR   PIR; T07133; T07133.
DR   AlphaFoldDB; P56335; -.
DR   SMR; P56335; -.
DR   STRING; 4113.PGSC0003DMT400028926; -.
DR   EnsemblPlants; PGSC0003DMT400028926; PGSC0003DMT400028926; PGSC0003DMG400011137.
DR   EnsemblPlants; RHC07H1G0196.2.1; RHC07H1G0196.2.1; RHC07H1G0196.2.
DR   Gramene; PGSC0003DMT400028926; PGSC0003DMT400028926; PGSC0003DMG400011137.
DR   Gramene; RHC07H1G0196.2.1; RHC07H1G0196.2.1; RHC07H1G0196.2.
DR   eggNOG; KOG3271; Eukaryota.
DR   HOGENOM; CLU_102600_1_0_1; -.
DR   InParanoid; P56335; -.
DR   OMA; TECIITH; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P56335; baseline.
DR   GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0045901; P:positive regulation of translational elongation; IBA:GO_Central.
DR   GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR001884; IF5A-like.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR   InterPro; IPR020189; Transl_elong_IF5A_C.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR11673; PTHR11673; 1.
DR   Pfam; PF01287; eIF-5a; 1.
DR   PIRSF; PIRSF003025; eIF5A; 1.
DR   SMART; SM01376; eIF-5a; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00037; eIF_5A; 1.
DR   PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE   2: Evidence at transcript level;
KW   Hypusine; Initiation factor; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..160
FT                   /note="Eukaryotic translation initiation factor 5A-3"
FT                   /id="PRO_0000142479"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         52
FT                   /note="Hypusine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9XI91"
SQ   SEQUENCE   160 AA;  17571 MW;  019C68B90F0CA9BF CRC64;
     MSDEEHHFES KADAGASKTY PQQAGTIRKN GYIVIKGRPC KVVEVSTSKT GKHGHAKCHF
     VAIDIFNGKK LEDIVPSSHN CDVPHVNRTD YQLIDISEDG FVSLLTESGN TKDDLRLPTD
     ESLLKQVKDG FQEGKDLVVS VMSAMGEEQI NAIKDIGTKN