ID   IF5A_DICDI              Reviewed;         159 AA.
AC   P13651; Q54NT9;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   03-AUG-2022, entry version 139.
DE   RecName: Full=Eukaryotic translation initiation factor 5A;
DE            Short=eIF-5A;
DE   AltName: Full=eIF-4D;
GN   Name=eif5a; Synonyms=eifE; ORFNames=DDB_G0284861;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-39; 53-72 AND 93-140,
RP   AND HYPUSINE AT LYS-55.
RC   STRAIN=AX3;
RX   PubMed=2540042; DOI=10.1016/0014-5793(89)80260-1;
RA   Sandholzer U., Centea-Intemann M., Noegel A.A., Lottspeich F.;
RT   "cDNA and derived amino acid sequence of the hypusine containing protein
RT   from Dictyostelium discoideum.";
RL   FEBS Lett. 246:94-100(1989).
RN   [2]
RP   SEQUENCE REVISION TO 12-16.
RA   Lottspeich F.;
RL   Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: mRNA-binding protein involved in translation elongation. Has
CC       an important function at the level of mRNA turnover, probably acting
CC       downstream of decapping. Critical for the efficient synthesis of
CC       peptide bonds between consecutive proline residues. Can resolve
CC       ribosomal stalling caused by consecutive prolines during translation
CC       (By similarity). Involved in actin dynamics and cell cycle progression,
CC       mRNA decay and probably in a pathway involved in stress response and
CC       maintenance of cell wall integrity. Functions as a regulator of
CC       apoptosis (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:P63241}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Lys-55 undergoes hypusination, a unique post-translational
CC       modification that consists in the addition of a butylamino group from
CC       spermidine to lysine side chain, leading to the formation of the
CC       unusual amino acid hypusine. eIF-5As are the only known proteins to
CC       undergo this modification, which is essential for their function.
CC       {ECO:0000269|PubMed:2540042}.
CC   -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA33095.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X14970; CAA33095.1; ALT_INIT; mRNA.
DR   EMBL; AAFI02000073; EAL64894.1; -; Genomic_DNA.
DR   PIR; S03886; FIDOA.
DR   RefSeq; XP_639978.1; XM_634886.1.
DR   AlphaFoldDB; P13651; -.
DR   SMR; P13651; -.
DR   STRING; 44689.DDB0191442; -.
DR   PaxDb; P13651; -.
DR   EnsemblProtists; EAL64894; EAL64894; DDB_G0284861.
DR   GeneID; 8624890; -.
DR   KEGG; ddi:DDB_G0284861; -.
DR   dictyBase; DDB_G0284861; eif5a.
DR   eggNOG; KOG3271; Eukaryota.
DR   HOGENOM; CLU_102600_0_0_1; -.
DR   InParanoid; P13651; -.
DR   OMA; VFRNEYQ; -.
DR   PhylomeDB; P13651; -.
DR   Reactome; R-DDI-204626; Hypusine synthesis from eIF5A-lysine.
DR   PRO; PR:P13651; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR   GO; GO:0045901; P:positive regulation of translational elongation; IBA:GO_Central.
DR   GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR001884; IF5A-like.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014722; Rib_L2_dom2.
DR   InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR   InterPro; IPR020189; Transl_elong_IF5A_C.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   PANTHER; PTHR11673; PTHR11673; 1.
DR   Pfam; PF01287; eIF-5a; 1.
DR   PIRSF; PIRSF003025; eIF5A; 1.
DR   SMART; SM01376; eIF-5a; 1.
DR   SUPFAM; SSF50104; SSF50104; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00037; eIF_5A; 1.
DR   PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Elongation factor; Hypusine;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..159
FT                   /note="Eukaryotic translation initiation factor 5A"
FT                   /id="PRO_0000142458"
FT   MOD_RES         55
FT                   /note="Hypusine"
FT                   /evidence="ECO:0000269|PubMed:2540042"
SQ   SEQUENCE   159 AA;  17090 MW;  73C0300E5403EEAA CRC64;
     MSDNEALDVE DYAQAGSGAS LTFPIQCSAL RKNGFVVIKG FPCKIVDMST SKTGKHGHAK
     VNITAIDIFT GKKYEEICPS THNIDVPNVS RKEYTVMDVQ DGYLSLLDAG GEVKEDLALP
     EDDIGKEITQ MLKEGKEPLV SVISALGKEG VVSVKVSNN