IF5A_MAIZE
ID IF5A_MAIZE Reviewed; 160 AA.
AC P80639; P56332;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Eukaryotic translation initiation factor 5A;
DE Short=eIF-5A;
DE AltName: Full=eIF-4D;
GN Name=TIF5A;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. B73;
RA Korn A.M., Baysdorfer C.;
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. A188;
RA Cordts S., Loerz H., Dresselhaus T.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 71-78 AND 118-129.
RC TISSUE=Coleoptile;
RX AGRICOLA=IND20551642; DOI=10.1007/BF00224104;
RA Touzet P., Riccardi F., Morin C., Damerval C., Huet J.-C., Pernollet J.-C.,
RA Zivy M., de Vienne D.;
RT "The maize two dimensional gel protein database: towards an integrated
RT genome analysis program.";
RL Theor. Appl. Genet. 93:997-1005(1996).
CC -!- FUNCTION: The precise role of eIF-5A in protein biosynthesis is not
CC known but it functions by promoting the formation of the first peptide
CC bond.
CC -!- PTM: Lys-53 undergoes hypusination, a unique post-translational
CC modification that consists in the addition of a butylamino group from
CC spermidine to lysine side chain, leading to the formation of the
CC unusual amino acid hypusine. eIF-5As are the only known proteins to
CC undergo this modification, which is essential for their function.
CC {ECO:0000250|UniProtKB:Q9XI91}.
CC -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
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DR EMBL; AF034943; AAB88614.1; -; mRNA.
DR EMBL; Y07920; CAA69225.1; -; mRNA.
DR PIR; T01355; T01355.
DR RefSeq; NP_001105550.1; NM_001112080.1.
DR RefSeq; NP_001105606.1; NM_001112136.1.
DR AlphaFoldDB; P80639; -.
DR SMR; P80639; -.
DR STRING; 4577.GRMZM2G113696_P01; -.
DR iPTMnet; P80639; -.
DR PaxDb; P80639; -.
DR EnsemblPlants; Zm00001eb108410_T001; Zm00001eb108410_P001; Zm00001eb108410.
DR EnsemblPlants; Zm00001eb326630_T002; Zm00001eb326630_P002; Zm00001eb326630.
DR GeneID; 542537; -.
DR GeneID; 542601; -.
DR Gramene; Zm00001eb108410_T001; Zm00001eb108410_P001; Zm00001eb108410.
DR Gramene; Zm00001eb326630_T002; Zm00001eb326630_P002; Zm00001eb326630.
DR KEGG; zma:542537; -.
DR KEGG; zma:542601; -.
DR MaizeGDB; 123964; -.
DR eggNOG; KOG3271; Eukaryota.
DR HOGENOM; CLU_102600_1_0_1; -.
DR OMA; TIMINIQ; -.
DR OrthoDB; 1370513at2759; -.
DR Proteomes; UP000007305; Chromosome 2.
DR Proteomes; UP000007305; Chromosome 7.
DR ExpressionAtlas; P80639; baseline and differential.
DR Genevisible; P80639; ZM.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0045901; P:positive regulation of translational elongation; IBA:GO_Central.
DR GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR001884; IF5A-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR InterPro; IPR020189; Transl_elong_IF5A_C.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11673; PTHR11673; 1.
DR Pfam; PF01287; eIF-5a; 1.
DR PIRSF; PIRSF003025; eIF5A; 1.
DR SMART; SM01376; eIF-5a; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00037; eIF_5A; 1.
DR PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hypusine; Initiation factor;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..160
FT /note="Eukaryotic translation initiation factor 5A"
FT /id="PRO_0000142471"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 53
FT /note="Hypusine"
FT /evidence="ECO:0000250|UniProtKB:Q9XI91"
SQ SEQUENCE 160 AA; 17497 MW; 7CCFADFB2EC09CAD CRC64;
MSDSEEHHFE SKADAGASKT YPQQAGTVRK NGFIVIKNRP CKVVEVSTSK TGKHGHAKCH
FVAIDIFNGK KLEDIVPSSH NCDIPHVNRT EYQLIDISED GFVSLLTSDG NTKDDLRLPT
DETLVAQIKE GFESGKDLVV TVQSAMGEEQ ICALKDVGPK
