IF5A_PYRAE
ID IF5A_PYRAE Reviewed; 138 AA.
AC P56635;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Translation initiation factor 5A;
DE AltName: Full=Hypusine-containing protein;
DE AltName: Full=eIF-5A;
GN Name=eif5a; OrderedLocusNames=PAE3337;
OS Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS 104966 / NBRC 100827 / IM2).
OC Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Pyrobaculum.
OX NCBI_TaxID=178306;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX PubMed=11792869; DOI=10.1073/pnas.241636498;
RA Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA Miller J.H.;
RT "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT aerophilum.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), AND HYPUSINE AT LYS-42.
RX PubMed=9753699; DOI=10.1016/s0969-2126(98)00120-8;
RA Peat T.S., Newman J., Waldo G.S., Berendzen J., Terwilliger T.C.;
RT "Structure of translation initiation factor 5A from Pyrobaculum aerophilum
RT at 1.75-A resolution.";
RL Structure 6:1207-1214(1998).
CC -!- FUNCTION: Functions by promoting the formation of the first peptide
CC bond.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL64850.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE009441; AAL64850.1; ALT_INIT; Genomic_DNA.
DR PDB; 1BKB; X-ray; 1.75 A; A=4-138.
DR PDBsum; 1BKB; -.
DR AlphaFoldDB; P56635; -.
DR SMR; P56635; -.
DR STRING; 178306.PAE3337; -.
DR EnsemblBacteria; AAL64850; AAL64850; PAE3337.
DR KEGG; pai:PAE3337; -.
DR PATRIC; fig|178306.9.peg.2514; -.
DR eggNOG; arCOG04277; Archaea.
DR HOGENOM; CLU_102600_3_0_2; -.
DR InParanoid; P56635; -.
DR OMA; QIMDMET; -.
DR EvolutionaryTrace; P56635; -.
DR Proteomes; UP000002439; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045901; P:positive regulation of translational elongation; IBA:GO_Central.
DR GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00085; eIF_5A; 1.
DR InterPro; IPR001884; IF5A-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR InterPro; IPR022847; Transl_elong_IF5A_arc.
DR InterPro; IPR020189; Transl_elong_IF5A_C.
DR InterPro; IPR013185; Transl_elong_KOW-like.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11673; PTHR11673; 1.
DR Pfam; PF08207; EFP_N; 1.
DR Pfam; PF01287; eIF-5a; 1.
DR PIRSF; PIRSF003025; eIF5A; 1.
DR SMART; SM01376; eIF-5a; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00037; eIF_5A; 1.
DR PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hypusine; Initiation factor; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..138
FT /note="Translation initiation factor 5A"
FT /id="PRO_0000142499"
FT MOD_RES 42
FT /note="Hypusine"
FT /evidence="ECO:0000269|PubMed:9753699"
FT STRAND 10..13
FT /evidence="ECO:0007829|PDB:1BKB"
FT HELIX 14..16
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 22..25
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 28..38
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 47..54
FT /evidence="ECO:0007829|PDB:1BKB"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 69..73
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 77..86
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:1BKB"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:1BKB"
FT HELIX 106..108
FT /evidence="ECO:0007829|PDB:1BKB"
FT HELIX 111..114
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 122..128
FT /evidence="ECO:0007829|PDB:1BKB"
FT STRAND 131..137
FT /evidence="ECO:0007829|PDB:1BKB"
SQ SEQUENCE 138 AA; 15411 MW; E44997F8AE912E9A CRC64;
MVLKWVMSTK YVEAGELKEG SYVVIDGEPC RVVEIEKSKT GKHGSAKARI VAVGVFDGGK
RTLSLPVDAQ VEVPIIEKFT AQILSVSGDV IQLMDMRDYK TIEVPMKYVE EEAKGRLAPG
AEVEVWQILD RYKIIRVK
