IF5A_PYRHO
ID IF5A_PYRHO Reviewed; 138 AA.
AC O50089;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Translation initiation factor 5A;
DE AltName: Full=Hypusine-containing protein;
DE AltName: Full=PhoIF-5A;
DE AltName: Full=eIF-5A;
GN Name=eif5a; OrderedLocusNames=PH1381;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND HYPUSINE AT LYS-37.
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=12761201; DOI=10.1093/jb/mvg011;
RA Yao M., Ohsawa A., Kikukawa S., Tanaka I., Kimura M.;
RT "Crystal structure of hyperthermophilic archaeal initiation factor 5A: a
RT homologue of eukaryotic initiation factor 5A (eIF-5A).";
RL J. Biochem. 133:75-81(2003).
CC -!- FUNCTION: Functions by promoting the formation of the first peptide
CC bond. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
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DR EMBL; BA000001; BAA30487.1; -; Genomic_DNA.
DR PIR; G71010; G71010.
DR RefSeq; WP_010885470.1; NC_000961.1.
DR PDB; 1IZ6; X-ray; 2.00 A; A/B/C=1-138.
DR PDBsum; 1IZ6; -.
DR AlphaFoldDB; O50089; -.
DR SMR; O50089; -.
DR STRING; 70601.3257804; -.
DR EnsemblBacteria; BAA30487; BAA30487; BAA30487.
DR GeneID; 1443708; -.
DR KEGG; pho:PH1381; -.
DR eggNOG; arCOG04277; Archaea.
DR OMA; QIMDMET; -.
DR OrthoDB; 98221at2157; -.
DR EvolutionaryTrace; O50089; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045901; P:positive regulation of translational elongation; IEA:InterPro.
DR GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00085; eIF_5A; 1.
DR InterPro; IPR001884; IF5A-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR InterPro; IPR022847; Transl_elong_IF5A_arc.
DR InterPro; IPR020189; Transl_elong_IF5A_C.
DR InterPro; IPR013185; Transl_elong_KOW-like.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11673; PTHR11673; 1.
DR Pfam; PF08207; EFP_N; 1.
DR Pfam; PF01287; eIF-5a; 1.
DR PIRSF; PIRSF003025; eIF5A; 1.
DR SMART; SM01376; eIF-5a; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00037; eIF_5A; 1.
DR PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hypusine; Initiation factor; Protein biosynthesis.
FT CHAIN 1..138
FT /note="Translation initiation factor 5A"
FT /id="PRO_0000142501"
FT MOD_RES 37
FT /note="Hypusine"
FT /evidence="ECO:0000269|PubMed:12761201"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:1IZ6"
FT HELIX 9..11
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 16..20
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 23..32
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 42..49
FT /evidence="ECO:0007829|PDB:1IZ6"
FT TURN 50..52
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 55..61
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 64..69
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 72..81
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 83..89
FT /evidence="ECO:0007829|PDB:1IZ6"
FT TURN 91..93
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 96..100
FT /evidence="ECO:0007829|PDB:1IZ6"
FT HELIX 101..104
FT /evidence="ECO:0007829|PDB:1IZ6"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:1IZ6"
FT TURN 110..112
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 118..124
FT /evidence="ECO:0007829|PDB:1IZ6"
FT STRAND 127..133
FT /evidence="ECO:0007829|PDB:1IZ6"
SQ SEQUENCE 138 AA; 15285 MW; B736249262E129B0 CRC64;
MGDKTKVQVS KLKPGRYIII DDEPCRIVNI TVSSPGKHGS AKARIEAVGI FDGKVRSIVK
PTSAEVDVPI IDKKTAQVIA ITPDTVQIMD METYETFEVP IDTGVADEIR DQLKEGINVE
YWETLGRIKI MRIKGEGE
