IF5A_SPOFR
ID IF5A_SPOFR Reviewed; 160 AA.
AC P62925; Q9TVJ8;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Eukaryotic translation initiation factor 5A;
DE Short=eIF-5A;
GN Name=eIF-5A; Synonyms=eIF5A;
OS Spodoptera frugiperda (Fall armyworm).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC Noctuidae; Amphipyrinae; Spodoptera.
OX NCBI_TaxID=7108;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Midgut, and Ovary;
RX PubMed=10620048; DOI=10.1046/j.1365-2583.1999.00148.x;
RA van Oers M.M., van Marwijk M., Kwa M.S.G., Vlak J.M., Thomas A.A.M.;
RT "Cloning and analysis of cDNAs encoding the hypusine-containing protein
RT eIF5A of two lepidopteran insect species.";
RL Insect Mol. Biol. 8:531-538(1999).
CC -!- FUNCTION: mRNA-binding protein involved in translation elongation.
CC Critical for the efficient synthesis of peptide bonds between
CC consecutive proline residues. Can resolve ribosomal stalling caused by
CC consecutive prolines during translation (By similarity). Has an
CC important function at the level of mRNA turnover, probably acting
CC downstream of decapping. Involved in actin dynamics and cell cycle
CC progression, mRNA decay and probably in a pathway involved in stress
CC response and maintenance of cell wall integrity. Functions as a
CC regulator of apoptosis (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:P63241}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Lys-52 undergoes hypusination, a unique post-translational
CC modification that consists in the addition of a butylamino group from
CC spermidine to lysine side chain, leading to the formation of the
CC unusual amino acid hypusine. eIF-5As are the only known proteins to
CC undergo this modification, which is essential for their function.
CC {ECO:0000250|UniProtKB:P63241}.
CC -!- SIMILARITY: Belongs to the eIF-5A family. {ECO:0000305}.
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DR EMBL; AF109731; AAF13316.1; -; mRNA.
DR PDB; 5HY6; X-ray; 2.19 A; A=1-160.
DR PDBsum; 5HY6; -.
DR AlphaFoldDB; P62925; -.
DR SMR; P62925; -.
DR PRIDE; P62925; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0045901; P:positive regulation of translational elongation; IEA:InterPro.
DR GO; GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR001884; IF5A-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR014722; Rib_L2_dom2.
DR InterPro; IPR019769; Trans_elong_IF5A_hypusine_site.
DR InterPro; IPR020189; Transl_elong_IF5A_C.
DR InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR PANTHER; PTHR11673; PTHR11673; 1.
DR Pfam; PF01287; eIF-5a; 1.
DR PIRSF; PIRSF003025; eIF5A; 1.
DR SMART; SM01376; eIF-5a; 1.
DR SUPFAM; SSF50104; SSF50104; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00037; eIF_5A; 1.
DR PROSITE; PS00302; IF5A_HYPUSINE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Elongation factor; Hypusine; Protein biosynthesis;
KW RNA-binding.
FT CHAIN 1..160
FT /note="Eukaryotic translation initiation factor 5A"
FT /id="PRO_0000142462"
FT MOD_RES 52
FT /note="Hypusine"
FT /evidence="ECO:0000250|UniProtKB:P63241"
FT STRAND 18..23
FT /evidence="ECO:0007829|PDB:5HY6"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 32..35
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 38..47
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 58..64
FT /evidence="ECO:0007829|PDB:5HY6"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 70..75
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 77..84
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 87..96
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 100..105
FT /evidence="ECO:0007829|PDB:5HY6"
FT HELIX 120..131
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 135..143
FT /evidence="ECO:0007829|PDB:5HY6"
FT STRAND 146..155
FT /evidence="ECO:0007829|PDB:5HY6"
SQ SEQUENCE 160 AA; 17525 MW; D3CE1E65CEA21732 CRC64;
MADIEDTHFE TGDSGASATF PMQCSALRKN GFVMLKGRPC KIVEMSTSKT GKHGHAKVHL
VGIDIFNGKK YEDICPSTHN MDVPHVKRED YQLTDISDDG YLTLMADNGD LREDLKIPDG
DLGTQLRSDF DSGKELLCTV LKSCGEECVI AVKANTALDK
