IF61_ARATH
ID IF61_ARATH Reviewed; 247 AA.
AC O22290;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Eukaryotic translation initiation factor 6-1 {ECO:0000255|HAMAP-Rule:MF_03132};
DE Short=AteIF-6;1;
GN Name=EIF6-1 {ECO:0000255|HAMAP-Rule:MF_03132}; OrderedLocusNames=At2g39820;
GN ORFNames=T5I7.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20570652; DOI=10.1016/j.bbrc.2010.06.001;
RA Kato Y., Konishi M., Shigyo M., Yoneyama T., Yanagisawa S.;
RT "Characterization of plant eukaryotic translation initiation factor 6
RT (eIF6) genes: The essential role in embryogenesis and their differential
RT expression in Arabidopsis and rice.";
RL Biochem. Biophys. Res. Commun. 397:673-678(2010).
CC -!- FUNCTION: Binds to the 60S ribosomal subunit and prevents its
CC association with the 40S ribosomal subunit to form the 80S initiation
CC complex in the cytoplasm. May also be involved in ribosome biogenesis.
CC {ECO:0000255|HAMAP-Rule:MF_03132, ECO:0000269|PubMed:20570652}.
CC -!- SUBUNIT: Monomer. Associates with the 60S ribosomal subunit.
CC {ECO:0000255|HAMAP-Rule:MF_03132}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03132}.
CC Nucleus, nucleolus {ECO:0000255|HAMAP-Rule:MF_03132}. Note=Shuttles
CC between cytoplasm and nucleus/nucleolus. {ECO:0000255|HAMAP-
CC Rule:MF_03132}.
CC -!- DISRUPTION PHENOTYPE: Causes embryonic lethality.
CC {ECO:0000269|PubMed:20570652}.
CC -!- SIMILARITY: Belongs to the eIF-6 family. {ECO:0000255|HAMAP-
CC Rule:MF_03132}.
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DR EMBL; AC003000; AAB87131.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09735.1; -; Genomic_DNA.
DR EMBL; BX821100; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T01012; T01012.
DR RefSeq; NP_181512.1; NM_129541.3.
DR AlphaFoldDB; O22290; -.
DR SMR; O22290; -.
DR BioGRID; 3907; 1.
DR STRING; 3702.AT2G39820.1; -.
DR PaxDb; O22290; -.
DR PRIDE; O22290; -.
DR ProteomicsDB; 228736; -.
DR EnsemblPlants; AT2G39820.1; AT2G39820.1; AT2G39820.
DR GeneID; 818569; -.
DR Gramene; AT2G39820.1; AT2G39820.1; AT2G39820.
DR KEGG; ath:AT2G39820; -.
DR Araport; AT2G39820; -.
DR TAIR; locus:2063927; AT2G39820.
DR eggNOG; KOG3185; Eukaryota.
DR HOGENOM; CLU_071894_0_0_1; -.
DR InParanoid; O22290; -.
DR OMA; GNAIACN; -.
DR OrthoDB; 1157302at2759; -.
DR PhylomeDB; O22290; -.
DR PRO; PR:O22290; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22290; baseline and differential.
DR Genevisible; O22290; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:1902626; P:assembly of large subunit precursor of preribosome; IBA:GO_Central.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IBA:GO_Central.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IBA:GO_Central.
DR CDD; cd00527; IF6; 1.
DR HAMAP; MF_00032; eIF_6; 1.
DR InterPro; IPR002769; eIF6.
DR PANTHER; PTHR10784; PTHR10784; 1.
DR Pfam; PF01912; eIF-6; 1.
DR PIRSF; PIRSF006413; IF-6; 1.
DR SMART; SM00654; eIF6; 1.
DR TIGRFAMs; TIGR00323; eIF-6; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Initiation factor; Nucleus; Protein biosynthesis;
KW Reference proteome; Ribosome biogenesis.
FT CHAIN 1..247
FT /note="Eukaryotic translation initiation factor 6-1"
FT /id="PRO_0000153739"
FT CONFLICT 164
FT /note="H -> N (in Ref. 3; BX821100)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 247 AA; 26511 MW; A12A7099700B9A06 CRC64;
MATRLQYDNN NCEIGVFSKL TNAYCLVSAT SASANFFTGY ESKLKGVIPI VTTSIGGSGT
IGSLCVGNKN GLLLSHTITD QELQHLRDSL PDEVVVQRIE EPICALGNAI ACNDYVALVH
PKLEKDTEEI ISDVLGVEVY RQTIANNELV GSYCSLSNNG GMVHSNTNVE EMVELANLVQ
VPLVAGTVNR GSQVISAGLT VNDWTAFCGS DTTAVELSVV NNIFKLVQSQ PDFVGSEMRK
SLIDTYV
