IF6_BOVIN
ID IF6_BOVIN Reviewed; 245 AA.
AC Q9TU47; Q2TBM1;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132};
DE Short=eIF-6 {ECO:0000255|HAMAP-Rule:MF_03132};
DE AltName: Full=Imc-415 homolog;
GN Name=EIF6 {ECO:0000255|HAMAP-Rule:MF_03132};
GN Synonyms=ITGB4BP {ECO:0000255|HAMAP-Rule:MF_03132};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Holstein; TISSUE=Mammary gland;
RX PubMed=10879479; DOI=10.1271/bbb.64.1052;
RA Ha S., Baik M., Choi Y.;
RT "Cloning and expression of bovine imc-415 cDNA from mammary gland.";
RL Biosci. Biotechnol. Biochem. 64:1052-1054(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Binds to the 60S ribosomal subunit and prevents its
CC association with the 40S ribosomal subunit to form the 80S initiation
CC complex in the cytoplasm. Behaves as a stimulatory translation
CC initiation factor downstream insulin/growth factors. Is also involved
CC in ribosome biogenesis. Associates with pre-60S subunits in the nucleus
CC and is involved in its nuclear export. Cytoplasmic release of TIF6 from
CC 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-
CC dependent protein kinase C activity (By similarity). In tissues
CC responsive to insulin, controls fatty acid synthesis and glycolysis by
CC exerting translational control of adipogenic transcription factors such
CC as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR.
CC Required for ROS-dependent megakaryocyte maturation and platelets
CC formation, controls the expression of mitochondrial respiratory chain
CC genes involved in reactive oxygen species (ROS) synthesis (By
CC similarity). Involved in miRNA-mediated gene silencing by the RNA-
CC induced silencing complex (RISC). Required for both miRNA-mediated
CC translational repression and miRNA-mediated cleavage of complementary
CC mRNAs by RISC (By similarity). Modulates cell cycle progression and
CC global translation of pre-B cells, its activation seems to be rate-
CC limiting in tumorigenesis and tumor growth (By similarity).
CC {ECO:0000250|UniProtKB:O55135, ECO:0000250|UniProtKB:P56537,
CC ECO:0000255|HAMAP-Rule:MF_03132}.
CC -!- SUBUNIT: Monomer. Associates with the 60S ribosomal subunit. Interacts
CC with RACK1 (By similarity). Interacts with DICER1, AGO2, TARBP2, MOV10
CC and RPL7A; they form a large RNA-induced silencing complex (RISC) (By
CC similarity). {ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
CC Rule:MF_03132}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03132}.
CC Nucleus, nucleolus {ECO:0000255|HAMAP-Rule:MF_03132}. Note=Shuttles
CC between cytoplasm and nucleus/nucleolus. {ECO:0000255|HAMAP-
CC Rule:MF_03132}.
CC -!- PTM: Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes
CC nuclear export. {ECO:0000250|UniProtKB:P56537}.
CC -!- PTM: Ufmylated by UFL1. {ECO:0000250|UniProtKB:O55135}.
CC -!- SIMILARITY: Belongs to the eIF-6 family. {ECO:0000255|HAMAP-
CC Rule:MF_03132}.
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DR EMBL; AF141872; AAF00595.1; -; mRNA.
DR EMBL; BC109922; AAI09923.1; -; mRNA.
DR PIR; JC7273; JC7273.
DR RefSeq; NP_777255.1; NM_174830.1.
DR RefSeq; XP_005214560.1; XM_005214503.3.
DR AlphaFoldDB; Q9TU47; -.
DR SMR; Q9TU47; -.
DR IntAct; Q9TU47; 2.
DR STRING; 9913.ENSBTAP00000014966; -.
DR PaxDb; Q9TU47; -.
DR PRIDE; Q9TU47; -.
DR Ensembl; ENSBTAT00000076570; ENSBTAP00000062338; ENSBTAG00000011263.
DR GeneID; 286811; -.
DR KEGG; bta:286811; -.
DR CTD; 3692; -.
DR VEuPathDB; HostDB:ENSBTAG00000011263; -.
DR VGNC; VGNC:57006; EIF6.
DR eggNOG; KOG3185; Eukaryota.
DR GeneTree; ENSGT00390000015972; -.
DR HOGENOM; CLU_071894_0_0_1; -.
DR InParanoid; Q9TU47; -.
DR OMA; GEDTTGP; -.
DR OrthoDB; 1157302at2759; -.
DR TreeFam; TF105396; -.
DR Proteomes; UP000009136; Chromosome 13.
DR Bgee; ENSBTAG00000011263; Expressed in esophagus and 107 other tissues.
DR ExpressionAtlas; Q9TU47; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:1902626; P:assembly of large subunit precursor of preribosome; IBA:GO_Central.
DR GO; GO:0000460; P:maturation of 5.8S rRNA; IBA:GO_Central.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0035195; P:miRNA-mediated gene silencing; ISS:UniProtKB.
DR GO; GO:0035278; P:miRNA-mediated gene silencing by inhibition of translation; ISS:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
DR GO; GO:0042304; P:regulation of fatty acid biosynthetic process; ISS:UniProtKB.
DR GO; GO:0006110; P:regulation of glycolytic process; ISS:UniProtKB.
DR GO; GO:0045652; P:regulation of megakaryocyte differentiation; ISS:UniProtKB.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; ISS:UniProtKB.
DR GO; GO:0032868; P:response to insulin; ISS:UniProtKB.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IBA:GO_Central.
DR CDD; cd00527; IF6; 1.
DR HAMAP; MF_00032; eIF_6; 1.
DR InterPro; IPR002769; eIF6.
DR PANTHER; PTHR10784; PTHR10784; 1.
DR Pfam; PF01912; eIF-6; 1.
DR PIRSF; PIRSF006413; IF-6; 1.
DR SMART; SM00654; eIF6; 1.
DR TIGRFAMs; TIGR00323; eIF-6; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Initiation factor; Nucleus; Phosphoprotein;
KW Protein biosynthesis; Reference proteome; Ubl conjugation.
FT CHAIN 1..245
FT /note="Eukaryotic translation initiation factor 6"
FT /id="PRO_0000259426"
FT MOD_RES 113
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P56537"
FT MOD_RES 165
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O55135, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O55135, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 174
FT /note="Phosphoserine; by CK1"
FT /evidence="ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 175
FT /note="Phosphoserine; by CK1"
FT /evidence="ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 235
FT /note="Phosphoserine; by PKC"
FT /evidence="ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 239
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT MOD_RES 243
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P56537, ECO:0000255|HAMAP-
FT Rule:MF_03132"
FT CONFLICT 141
FT /note="T -> K (in Ref. 1; AAF00595)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="V -> A (in Ref. 1; AAF00595)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="T -> A (in Ref. 1; AAF00595)"
FT /evidence="ECO:0000305"
FT CONFLICT 222..224
FT /note="FKL -> SKP (in Ref. 1; AAF00595)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="I -> T (in Ref. 1; AAF00595)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 26513 MW; C62D76AD25402243 CRC64;
MAVRASFENN CEIGCFAKLT NSYCLVAIGG SENFYSVFEG ELAGTIPVVH ASIAGCRIIG
RMCVGNRHGL LVPNNTTDQE LQHIRNCLPD SVQIRRVEER LSALGNVTTC NDYVALVHPD
LDRETEEILA DVLKVEVFRQ TVADQVLVGS YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP
LVAGTVNRGS EVIAAGMVVN DWCAFCGLDT TSTELSVVES VFKLNEAQPS TIATSMRDSL
IDSLT
