IFA3_CAEEL
ID IFA3_CAEEL Reviewed; 581 AA.
AC Q21065; Q20671; Q94367;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Intermediate filament protein ifa-3;
DE AltName: Full=Cel IF A3;
DE AltName: Full=Intermediate filament protein A3;
DE Short=IF-A3;
GN Name=ifa-3; ORFNames=F52E10.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=8013462; DOI=10.1002/j.1460-2075.1994.tb06553.x;
RA Dodemont H., Riemer D., Ledger T.N., Weber K.;
RT "Eight genes and alternative RNA processing pathways generate an
RT unexpectedly large diversity of cytoplasmic intermediate filament proteins
RT in the nematode Caenorhabditis elegans.";
RL EMBO J. 13:2625-2638(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11427699; DOI=10.1073/pnas.121169998;
RA Karabinos A., Schmidt H., Harborth J., Schnabel R., Weber K.;
RT "Essential roles for four cytoplasmic intermediate filament proteins in
RT Caenorhabditis elegans development.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7863-7868(2001).
RN [4]
RP INTERACTION WITH IFB-1.
RX PubMed=14529618; DOI=10.1016/j.jmb.2003.08.041;
RA Karabinos A., Schulze E., Schuenemann J., Parry D.A.D., Weber K.;
RT "In vivo and in vitro evidence that the four essential intermediate
RT filament (IF) proteins A1, A2, A3 and B1 of the nematode Caenorhabditis
RT elegans form an obligate heteropolymeric IF system.";
RL J. Mol. Biol. 333:307-319(2003).
RN [5]
RP FUNCTION.
RX PubMed=14975728; DOI=10.1016/j.ydbio.2003.11.007;
RA Woo W.-M., Goncharov A., Jin Y., Chisholm A.D.;
RT "Intermediate filaments are required for C. elegans epidermal elongation.";
RL Dev. Biol. 267:216-229(2004).
CC -!- FUNCTION: Cytoplasmic intermediate filaments provide mechanical
CC strength to cells. Essential protein, involved in attachment structures
CC in epidermal cells that connect muscles to the external cuticle.
CC Required for epidermal morphogenesis in embryos. Probable component of
CC embryonic epidermal attachment structures.
CC {ECO:0000269|PubMed:11427699, ECO:0000269|PubMed:14975728}.
CC -!- SUBUNIT: Forms some heteromeric filaments with ifb-1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11427699}.
CC -!- TISSUE SPECIFICITY: Expressed in the embryonic and larval hypodermis.
CC Also expressed in the ventral nerve cord of larvae.
CC {ECO:0000269|PubMed:11427699}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos and larvae. Not expressed in
CC adults.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; X70831; CAA50179.1; -; Genomic_DNA.
DR EMBL; Z54282; CAA91057.4; -; Genomic_DNA.
DR EMBL; Z81140; CAA91057.4; JOINED; Genomic_DNA.
DR PIR; S46327; S46327.
DR PIR; T22507; T22507.
DR RefSeq; NP_510649.3; NM_078248.7.
DR AlphaFoldDB; Q21065; -.
DR SMR; Q21065; -.
DR BioGRID; 46585; 1.
DR STRING; 6239.F52E10.5; -.
DR iPTMnet; Q21065; -.
DR EPD; Q21065; -.
DR PaxDb; Q21065; -.
DR PeptideAtlas; Q21065; -.
DR EnsemblMetazoa; F52E10.5.1; F52E10.5.1; WBGene00002051.
DR GeneID; 181699; -.
DR KEGG; cel:CELE_F52E10.5; -.
DR UCSC; F52E10.5; c. elegans.
DR CTD; 181699; -.
DR WormBase; F52E10.5; CE34875; WBGene00002051; ifa-3.
DR eggNOG; KOG0977; Eukaryota.
DR GeneTree; ENSGT00970000196730; -.
DR HOGENOM; CLU_012560_7_0_1; -.
DR InParanoid; Q21065; -.
DR OMA; SEYATHR; -.
DR OrthoDB; 701388at2759; -.
DR PhylomeDB; Q21065; -.
DR PRO; PR:Q21065; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00002051; Expressed in embryo and 3 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0005652; C:nuclear lamina; IBA:GO_Central.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; IBA:GO_Central.
DR GO; GO:0031507; P:heterochromatin assembly; IBA:GO_Central.
DR GO; GO:0006998; P:nuclear envelope organization; IBA:GO_Central.
DR GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR GO; GO:0051664; P:nuclear pore localization; IBA:GO_Central.
DR GO; GO:0090435; P:protein localization to nuclear envelope; IBA:GO_Central.
DR Gene3D; 2.60.40.1260; -; 1.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR016451; Intermed_filament_ifa/ifb.
DR InterPro; IPR001322; Lamin_tail_dom.
DR InterPro; IPR036415; Lamin_tail_dom_sf.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF00932; LTD; 1.
DR PIRSF; PIRSF005546; Intermed_filamnt_Ifb-2; 1.
DR SMART; SM01391; Filament; 1.
DR SUPFAM; SSF74853; SSF74853; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
DR PROSITE; PS51841; LTD; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Intermediate filament; Reference proteome.
FT CHAIN 1..581
FT /note="Intermediate filament protein ifa-3"
FT /id="PRO_0000063836"
FT DOMAIN 71..424
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT DOMAIN 457..574
FT /note="LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01187"
FT REGION 1..74
FT /note="Head"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..106
FT /note="Coil 1A"
FT REGION 107..120
FT /note="Linker 1"
FT REGION 121..258
FT /note="Coil 1B"
FT REGION 259..276
FT /note="Linker 12"
FT REGION 277..424
FT /note="Coil 2"
FT REGION 425..578
FT /note="Tail"
SQ SEQUENCE 581 AA; 66563 MW; ECF989B21E460C7F CRC64;
MADPDSYRSS ITSRPAFNRT VTSSTQNYGT PASGNRVLKI VTETHTSSVA SGLSPYGQGA
ASTIRDDRER EKKEITELND RLASYIGKVR FLAAQNRKLE ADLNVLQSRF GKSTGSVKIM
YEMEITTATN VVKETGKDHE EAEKEIGKIK DQLDELRKKF EEAQKGRAED RLKIDELLVT
LSNLEAEINL LKRRIALLEE EVARLKKENF RLTSELQRVR SELDQETLLR IDNQNKVTTI
LEEIDFMKRG FETELKDLQA QAARDTTSEN REYFKNELMN SIRDIRAEYD RFMAGNRNDL
ESWSQIRVQE INTQTNRQNA EINHKRDEVK RLHSQVSELK SKHAELAARN GLLEKQLEDL
NYQLEDDQRS YEAALNDKDA QVRKLREECQ ALLVELQMLL DTKQTLDGEL KVYRRMLEGN
SEENGLRQLV EKVVRTSAIN EEVDTETMRV VKGEHSSRTS YQRSAKGNVS IKEVSPEGKF
VILENTHRDK EEPLGDWKLK RKIDGKREIV FTFPSDYILH PVQTVKIFAR GNGVANPPEV
LVFEGDDTFG AGANVQTILY NNSGEERATH MQRQSQQTTT S
