IFA4_CAEEL
ID IFA4_CAEEL Reviewed; 575 AA.
AC P90900; Q9GYN4;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 3.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Intermediate filament protein ifa-4;
DE AltName: Full=Cel IF A4;
DE AltName: Full=Intermediate filament protein A4;
DE Short=IF-A4;
GN Name=ifa-4; ORFNames=K05B2.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=8013462; DOI=10.1002/j.1460-2075.1994.tb06553.x;
RA Dodemont H., Riemer D., Ledger T.N., Weber K.;
RT "Eight genes and alternative RNA processing pathways generate an
RT unexpectedly large diversity of cytoplasmic intermediate filament proteins
RT in the nematode Caenorhabditis elegans.";
RL EMBO J. 13:2625-2638(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=11427699; DOI=10.1073/pnas.121169998;
RA Karabinos A., Schmidt H., Harborth J., Schnabel R., Weber K.;
RT "Essential roles for four cytoplasmic intermediate filament proteins in
RT Caenorhabditis elegans development.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7863-7868(2001).
RN [4]
RP PROBABLE INTERACTION WITH IFB-1.
RX PubMed=14529618; DOI=10.1016/j.jmb.2003.08.041;
RA Karabinos A., Schulze E., Schuenemann J., Parry D.A.D., Weber K.;
RT "In vivo and in vitro evidence that the four essential intermediate
RT filament (IF) proteins A1, A2, A3 and B1 of the nematode Caenorhabditis
RT elegans form an obligate heteropolymeric IF system.";
RL J. Mol. Biol. 333:307-319(2003).
CC -!- FUNCTION: Cytoplasmic intermediate filaments provide mechanical
CC strength to cells. Non-essential protein.
CC {ECO:0000269|PubMed:11427699}.
CC -!- SUBUNIT: Probably forms some heteromeric filaments with ifb-1.
CC -!- INTERACTION:
CC P90900; P34288-2: pac-1; NbExp=3; IntAct=EBI-322249, EBI-11465290;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the larva in the pharyngeal-intestinal
CC valve, the rectum and some neurons of the tail. Highly expressed in the
CC intestine of the newly formed dauer larva. Colocalizes with ifb-1. Not
CC expressed in the intestine of normal developmental stages.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; X70833; CAA50181.1; -; Genomic_DNA.
DR EMBL; FO081589; CCD72670.2; -; Genomic_DNA.
DR PIR; S46329; S46329.
DR RefSeq; NP_508836.5; NM_076435.7.
DR AlphaFoldDB; P90900; -.
DR SMR; P90900; -.
DR BioGRID; 45700; 19.
DR DIP; DIP-24430N; -.
DR IntAct; P90900; 9.
DR MINT; P90900; -.
DR STRING; 6239.K05B2.3; -.
DR EPD; P90900; -.
DR PaxDb; P90900; -.
DR PeptideAtlas; P90900; -.
DR EnsemblMetazoa; K05B2.3.1; K05B2.3.1; WBGene00002052.
DR GeneID; 180765; -.
DR KEGG; cel:CELE_K05B2.3; -.
DR UCSC; K05B2.3; c. elegans.
DR CTD; 180765; -.
DR WormBase; K05B2.3; CE47884; WBGene00002052; ifa-4.
DR eggNOG; KOG0977; Eukaryota.
DR HOGENOM; CLU_012560_7_0_1; -.
DR InParanoid; P90900; -.
DR OMA; PPMRLHD; -.
DR OrthoDB; 701388at2759; -.
DR PhylomeDB; P90900; -.
DR SignaLink; P90900; -.
DR PRO; PR:P90900; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00002052; Expressed in larva and 3 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005882; C:intermediate filament; ISS:WormBase.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0005652; C:nuclear lamina; IBA:GO_Central.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; IBA:GO_Central.
DR GO; GO:0031507; P:heterochromatin assembly; IBA:GO_Central.
DR GO; GO:0006998; P:nuclear envelope organization; IBA:GO_Central.
DR GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR GO; GO:0051664; P:nuclear pore localization; IBA:GO_Central.
DR GO; GO:0090435; P:protein localization to nuclear envelope; IBA:GO_Central.
DR Gene3D; 2.60.40.1260; -; 1.
DR InterPro; IPR018039; IF_conserved.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR016451; Intermed_filament_ifa/ifb.
DR InterPro; IPR001322; Lamin_tail_dom.
DR InterPro; IPR036415; Lamin_tail_dom_sf.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF00932; LTD; 1.
DR PIRSF; PIRSF005546; Intermed_filamnt_Ifb-2; 1.
DR SMART; SM01391; Filament; 1.
DR SUPFAM; SSF74853; SSF74853; 1.
DR PROSITE; PS00226; IF_ROD_1; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
DR PROSITE; PS51841; LTD; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Intermediate filament; Reference proteome.
FT CHAIN 1..575
FT /note="Intermediate filament protein ifa-4"
FT /id="PRO_0000063837"
FT DOMAIN 70..423
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT DOMAIN 456..572
FT /note="LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01187"
FT REGION 1..73
FT /note="Head"
FT REGION 74..105
FT /note="Coil 1A"
FT REGION 106..119
FT /note="Linker 1"
FT REGION 120..257
FT /note="Coil 1B"
FT REGION 258..275
FT /note="Linker 12"
FT REGION 276..423
FT /note="Coil 2"
FT REGION 424..572
FT /note="Tail"
SQ SEQUENCE 575 AA; 66359 MW; FEF4CBA3E6EA9556 CRC64;
MSESEYRSSI SVRPTFNRTM QSTATYGSTI SSGTSRTLKI TEIGGSNLTS GMSPFGGHAA
SAIRESRTRE KKEMSELNDR LASYIEKVRF LEAQNRKMEK DLNLLRGKWG HDSTSVKVMY
ETELRSAKDL IADSDKERAQ LEDQIKKLVE ELNNYRNKLH EAERGADVTR KELDNVLGRL
GALEAEIELL KRRISLMEES IGHLKRENHR MIDSLHHARN AVEQETLNRI DYQNQVQTLL
EECDFIRRIH DSEIHDLLAM ASRDTTVENR EYFKNELSSA IREIRSEYDH VSNTTRTDIE
SWYKLKVQEI HTQNSRNCLE QNYAREEVKR LRTTLGDMRG KMADLEGRNL LLEKQIEDLN
YQMEEDMRSY EQSLNDKDTS INKLRDESKI LMVELQMLID TKQTLDAEIV IYRKMLDGEE
NRAGLRQLVE QVVKTTSIHQ TKEIESLRVL KGETTSHSSY SRSAKGNIAI QEAEPSGKYI
VLENISRRDE NIGDWKLRRK IAGKREIVFT FPREFTLRAQ KSVKIFARGQ GVHSPPDSLV
YDLEDSFGTG NDVVTTLYNK EGEDRASHSQ RASHT
