IFIT3_MOUSE
ID IFIT3_MOUSE Reviewed; 403 AA.
AC Q64345; Q99L62;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Interferon-induced protein with tetratricopeptide repeats 3;
DE Short=IFIT-3;
DE AltName: Full=Glucocorticoid-attenuated response gene 49 protein;
DE Short=GARG-49;
DE Short=P49;
DE AltName: Full=IRG2;
GN Name=Ifit3; Synonyms=Garg49, Ifi49, Isg49;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8660659; DOI=10.1006/abbi.1996.0256;
RA Smith J.B., Herschman H.R.;
RT "The glucocorticoid attenuated response genes GARG-16, GARG-39, and GARG-
RT 49/IRG2 encode inducible proteins containing multiple tetratricopeptide
RT repeat domains.";
RL Arch. Biochem. Biophys. 330:290-300(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=8207206;
RA Lee C.G.G., Demarquoy J., Jackson M.J., O'Brien W.E.;
RT "Molecular cloning and characterization of a murine LPS-inducible cDNA.";
RL J. Immunol. 152:5758-5767(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP REVIEW.
RX PubMed=20950130; DOI=10.1089/jir.2010.0101;
RA Fensterl V., Sen G.C.;
RT "The ISG56/IFIT1 gene family.";
RL J. Interferon Cytokine Res. 31:71-78(2011).
RN [6]
RP INDUCTION BY T.GONDII INFECTION.
RX PubMed=32731337; DOI=10.3390/microorganisms8081136;
RA Wang J., Liu T., Mahmmod Y.S., Yang Z., Tan J., Ren Z., Zhang X., Yang X.,
RA Zhang X.X., Yuan Z.G.;
RT "Transcriptome Analysis of Testes and Uterus: Reproductive Dysfunction
RT Induced by Toxoplasma gondii in Mice.";
RL Microorganisms 8:0-0(2020).
CC -!- FUNCTION: IFN-induced antiviral protein which acts as an inhibitor of
CC cellular as well as viral processes, cell migration, proliferation,
CC signaling, and viral replication. Enhances MAVS-mediated host antiviral
CC responses by serving as an adapter bridging TBK1 to MAVS which leads to
CC the activation of TBK1 and phosphorylation of IRF3 and phosphorylated
CC IRF3 translocates into nucleus to promote antiviral gene transcription.
CC Exhibits an antiproliferative activity via the up-regulation of cell
CC cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally,
CC CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in
CC the nucleus and exports it to the cytoplasm for ubiquitin-dependent
CC degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby
CC increasing nuclear CDKN1B/p27 protein levels. Up-regulates CDKN1A/p21
CC by down-regulating MYC, a repressor of CDKN1A/p21. Can negatively
CC regulate the apoptotic effects of IFIT2 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of an interferon-dependent multiprotein complex, at
CC least composed of IFIT1, IFIT2 and IFIT3 (By similarity). Interacts
CC with IFIT1 and IFIT2 (By similarity). Interacts (via N-terminus) with
CC MAVS, TBK1, TRAF6 and DDX58 (By similarity). Interacts with COPS5 (By
CC similarity). {ECO:0000250|UniProtKB:O14879}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O14879}.
CC Mitochondrion {ECO:0000250|UniProtKB:O14879}.
CC -!- INDUCTION: Induced by T.gondii infection in the testes and uterus.
CC {ECO:0000269|PubMed:32731337}.
CC -!- SIMILARITY: Belongs to the IFIT family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U43086; AAC52627.1; -; Genomic_DNA.
DR EMBL; L32974; AAA39329.1; -; mRNA.
DR EMBL; BC003804; AAH03804.1; -; mRNA.
DR CCDS; CCDS29762.1; -.
DR PIR; S71356; S71356.
DR RefSeq; NP_034631.1; NM_010501.2.
DR AlphaFoldDB; Q64345; -.
DR SMR; Q64345; -.
DR BioGRID; 200527; 11.
DR IntAct; Q64345; 1.
DR STRING; 10090.ENSMUSP00000099889; -.
DR iPTMnet; Q64345; -.
DR PhosphoSitePlus; Q64345; -.
DR MaxQB; Q64345; -.
DR PaxDb; Q64345; -.
DR PRIDE; Q64345; -.
DR ProteomicsDB; 266951; -.
DR DNASU; 15959; -.
DR Ensembl; ENSMUST00000102825; ENSMUSP00000099889; ENSMUSG00000074896.
DR GeneID; 15959; -.
DR KEGG; mmu:15959; -.
DR UCSC; uc008hgo.1; mouse.
DR CTD; 3437; -.
DR MGI; MGI:1101055; Ifit3.
DR VEuPathDB; HostDB:ENSMUSG00000074896; -.
DR eggNOG; KOG1124; Eukaryota.
DR GeneTree; ENSGT00950000182946; -.
DR HOGENOM; CLU_043482_0_0_1; -.
DR InParanoid; Q64345; -.
DR OMA; SHSVDCH; -.
DR OrthoDB; 460948at2759; -.
DR PhylomeDB; Q64345; -.
DR TreeFam; TF342671; -.
DR BioGRID-ORCS; 15959; 1 hit in 41 CRISPR screens.
DR ChiTaRS; Ifit3; mouse.
DR PRO; PR:Q64345; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q64345; protein.
DR Bgee; ENSMUSG00000074896; Expressed in right kidney and 53 other tissues.
DR ExpressionAtlas; Q64345; baseline and differential.
DR Genevisible; Q64345; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0035457; P:cellular response to interferon-alpha; IEA:InterPro.
DR GO; GO:0035458; P:cellular response to interferon-beta; IDA:MGI.
DR GO; GO:0051607; P:defense response to virus; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR GO; GO:0035634; P:response to stilbenoid; IEP:UniProtKB.
DR GO; GO:0009615; P:response to virus; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR024122; Interferon-induced_IFIT3.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR10271:SF3; PTHR10271:SF3; 1.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 5.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Cytoplasm; Immunity; Innate immunity; Mitochondrion;
KW Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..403
FT /note="Interferon-induced protein with tetratricopeptide
FT repeats 3"
FT /id="PRO_0000106350"
FT REPEAT 94..127
FT /note="TPR 1"
FT REPEAT 136..169
FT /note="TPR 2"
FT REPEAT 172..206
FT /note="TPR 3"
FT REPEAT 241..274
FT /note="TPR 4"
FT CONFLICT 158
FT /note="C -> Y (in Ref. 3; AAH03804)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 47223 MW; AE5F5ED42F4F8F60 CRC64;
MSEVNRESLE AILPQLKCHF TWNLFREGSM SSHMEDRVCN QVEHLNSEEK ATMYDLLAYI
KHLDGESKAA LECLGQAEDL RKSEHNDQSE IRRLVTWGNY AWIYYHMGRL SEAQAYVDKV
RQVCQKFANP YSMECPELEC EEGWTRLKCG RNERAKMCFE KALEEKPKDP ECSSGMAIAM
FRLEEKPEKQ FSVDALKQAM ELNPQNQYLK VLLALKLLRM GEEAEGERLI KDALGKAPNQ
TDVLQKAAQF YKKKGNLDRA IELLGKALRS TVNNSPLYSL VMCRYREILE QLQNKGDADS
SERRQRMAEL RRLTMEFMQK TLQRRRSPLN SYSDLIDFPE VERCYQMVIS KESPDVEEED
LYERYCNLQE YHRKSEDLAA LECLLQFPRN ERSIEKEEVK EQT
