APG_ARATH
ID APG_ARATH Reviewed; 534 AA.
AC P40602; Q93Z14; Q9LNT8;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Anther-specific proline-rich protein APG;
DE Flags: Precursor;
GN Name=APG; OrderedLocusNames=At1g20130; ORFNames=T20H2.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8401599; DOI=10.1046/j.1365-313x.1993.t01-5-00999.x;
RA Roberts M.R., Foster G.D., Blundell R.P., Robinson S.W., Kumar A.,
RA Draper J., Scott R.;
RT "Gametophytic and sporophytic expression of an anther-specific Arabidopsis
RT thaliana gene.";
RL Plant J. 3:111-120(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P40602-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Found in sporophytic and gametophytic cell types in
CC the anther, only in male fertile plants.
CC -!- DEVELOPMENTAL STAGE: Expressed in male gametogenesis, during microspore
CC development. Higher expression is found during microspore mitosis with
CC a dramatic decline during pollen maturation.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79900.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X60377; CAA42925.1; -; Genomic_DNA.
DR EMBL; AC022472; AAF79900.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29939.1; -; Genomic_DNA.
DR EMBL; AY058847; AAL24235.1; -; mRNA.
DR EMBL; BT008301; AAP37660.1; -; mRNA.
DR PIR; A86335; A86335.
DR PIR; S21961; S21961.
DR RefSeq; NP_173441.2; NM_101867.3. [P40602-1]
DR AlphaFoldDB; P40602; -.
DR SMR; P40602; -.
DR STRING; 3702.AT1G20130.1; -.
DR PaxDb; P40602; -.
DR PRIDE; P40602; -.
DR ProteomicsDB; 240884; -. [P40602-1]
DR EnsemblPlants; AT1G20130.1; AT1G20130.1; AT1G20130. [P40602-1]
DR GeneID; 838602; -.
DR Gramene; AT1G20130.1; AT1G20130.1; AT1G20130. [P40602-1]
DR KEGG; ath:AT1G20130; -.
DR Araport; AT1G20130; -.
DR TAIR; locus:2198636; AT1G20130.
DR eggNOG; ENOG502QSYS; Eukaryota.
DR InParanoid; P40602; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; P40602; -.
DR BioCyc; ARA:AT1G20130-MON; -.
DR PRO; PR:P40602; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P40602; baseline and differential.
DR GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR003882; Pistil_extensin.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PRINTS; PR01218; PSTLEXTENSIN.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..534
FT /note="Anther-specific proline-rich protein APG"
FT /id="PRO_0000017850"
FT REGION 59..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..199
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 211
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 508
FT /evidence="ECO:0000250"
FT ACT_SITE 511
FT /evidence="ECO:0000250"
FT CONFLICT 77
FT /note="S -> P (in Ref. 1; CAA42925)"
FT /evidence="ECO:0000305"
FT CONFLICT 141
FT /note="E -> A (in Ref. 1; CAA42925)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="E -> H (in Ref. 1; CAA42925)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 534 AA; 58008 MW; BA851DC3CF7429DB CRC64;
MKRSSLVDSC SYSRIFRSIF CLLSFCIFFL TTTNAQVMHR RLWPWPLWPR PYPQPWPMNP
PTPDPSPKPV APPGPSSKPV APPGPSPCPS PPPKPQPKPP PAPSPSPCPS PPPKPQPKPV
PPPACPPTPP KPQPKPAPPP EPKPAPPPAP KPVPCPSPPK PPAPTPKPVP PHGPPPKPAP
APTPAPSPKP APSPPKPENK TIPAVFFFGD SVFDTGNNNN LETKIKSNYR PYGMDFKFRV
ATGRFSNGMV ASDYLAKYMG VKEIVPAYLD PKIQPNDLLT GVSFASGGAG YNPTTSEAAN
AIPMLDQLTY FQDYIEKVNR LVRQEKSQYK LAGLEKTNQL ISKGVAIVVG GSNDLIITYF
GSGAQRLKND IDSYTTIIAD SAASFVLQLY GYGARRIGVI GTPPLGCVPS QRLKKKKICN
EELNYASQLF NSKLLLILGQ LSKTLPNSTF VYMDIYTIIS QMLETPAAYG FEETKKPCCK
TGLLSAGALC KKSTSKICPN TSSYLFWDGV HPTQRAYKTI NKVLIKEYLH VLSK
