APG_ORYSJ
ID APG_ORYSJ Reviewed; 505 AA.
AC Q6AT90; A0A0P0WHQ1; H7CE30;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Transcription factor APG;
DE AltName: Full=Basic helix-loop-helix protein 106;
DE Short=OsbHLH106;
DE AltName: Full=Protein ANTAGONIST OF PGL1;
DE AltName: Full=bHLH transcription factor bHLH106;
GN Name=APG; OrderedLocusNames=Os05g0139100, LOC_Os05g04740;
GN ORFNames=OSJNBa0069I13.9, OSJNBb0111O13.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP FUNCTION, INTERACTION WITH ILI6, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nipponbare; TISSUE=Grain;
RX PubMed=22363621; DOI=10.1371/journal.pone.0031325;
RA Heang D., Sassa H.;
RT "Antagonistic actions of HLH/bHLH proteins are involved in grain length and
RT weight in rice.";
RL PLoS ONE 7:E31325-E31325(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH ILI5.
RX PubMed=23136524; DOI=10.1270/jsbbs.62.133;
RA Heang D., Sassa H.;
RT "An atypical bHLH protein encoded by POSITIVE REGULATOR OF GRAIN LENGTH 2
RT is involved in controlling grain length and weight of rice through
RT interaction with a typical bHLH protein APG.";
RL Breed. Sci. 62:133-141(2012).
CC -!- FUNCTION: Atypical bHLH transcription factor that acts as negative
CC regulator of grain size. Binds the transcription factor ILI6 and forms
CC a heterodimer of antagonistic bHLH transcription factors that regulates
CC grain length and weight by controlling cell elongation in lemma and
CC palea. May be involved in the control of lamina inclination through
CC brassinosteroid signaling pathway. {ECO:0000269|PubMed:22363621}.
CC -!- SUBUNIT: Homodimer and heterodimer with ILI5 or ILI6.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:22363621}.
CC -!- MISCELLANEOUS: Plants silencing APG show enhanced bending of the lamina
CC joints and produce grains increased in size, caused by elongated cells
CC in lemma. {ECO:0000305|PubMed:22363621}.
CC -!- SIMILARITY: Belongs to the bHLH protein family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAL63287.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AB667900; BAL63287.1; ALT_SEQ; mRNA.
DR EMBL; AC137616; AAT77368.1; -; Genomic_DNA.
DR EMBL; AC137621; AAU10768.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16507.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92179.1; -; Genomic_DNA.
DR EMBL; AK287958; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015638282.1; XM_015782796.1.
DR AlphaFoldDB; Q6AT90; -.
DR SMR; Q6AT90; -.
DR STRING; 4530.OS05T0139100-01; -.
DR PRIDE; Q6AT90; -.
DR EnsemblPlants; Os05t0139100-01; Os05t0139100-01; Os05g0139100.
DR GeneID; 4337752; -.
DR Gramene; Os05t0139100-01; Os05t0139100-01; Os05g0139100.
DR KEGG; osa:4337752; -.
DR eggNOG; ENOG502QV9I; Eukaryota.
DR HOGENOM; CLU_014289_1_0_1; -.
DR InParanoid; Q6AT90; -.
DR OMA; LPPDYCA; -.
DR OrthoDB; 583799at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080113; P:regulation of seed growth; IMP:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR044273; PIF3-like.
DR PANTHER; PTHR46807; PTHR46807; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Growth regulation; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..505
FT /note="Transcription factor APG"
FT /id="PRO_0000429105"
FT DOMAIN 335..384
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 469..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..35
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..241
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 436
FT /note="Missing (in Ref. 6; AK287958)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 505 AA; 53029 MW; 2238FC6CF21B0632 CRC64;
MLRGNDTGSD LAELLWDNGA PAPLRPPPPP PFQPFTCSAA ATTSPPAHDY LFIKNLMRGG
GAANHHHHDD DDDDDDDVPW LHYHPVVDDD DDADADTAPL PPDYCAALLS GLSDHLPPPA
AAASRVDPDP CSSSHGAVVP STSAAAAKQA RTSGGGGGGV MNFTFFSRPL QQRPSGGETA
SASASAAATS TVPVESTVVQ AATNRLRSTP LFSDQRMAWL HPPKPSPRAA APPPPPPLAP
TTRHRLDTAA ATATVAQRLP PSEARAPDAP PPAATATATT SSVCSGNGDR RQLNWRDSHN
NQSAEWSASQ DELDLDDELA GVHRRSAARS SKRSRTAEVH NLSERRRRDR INEKMRALQE
LIPNCNKIDK ASMLEEAIEY LKTLQLQVQM MSMGTGMFVP PMMLPAAAAA MQHHHMQMQQ
MAGPMAAAAH FPHLGAAAAM GLAGFGMPAA AQFPCPMFPA APPMSMFAPP PPPPPFPHAA
ATAVEQTPSP PGAADAGNAP AVKQA
