ID   APH1B_DANRE             Reviewed;         258 AA.
AC   Q8JHE9;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Gamma-secretase subunit Aph-1b;
DE   AltName: Full=Anterior-pharynx-defective protein 1b;
GN   Name=aph1b; Synonyms=psfl;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12110170; DOI=10.1016/s1534-5807(02)00189-2;
RA   Francis R., McGrath G., Zhang J., Ruddy D.A., Sym M., Apfeld J., Nicoll M.,
RA   Maxwell M., Hai B., Ellis M.C., Parks A.L., Xu W., Li J., Gurney M.,
RA   Myers R.L., Himes C.S., Hiebsch R., Ruble C., Nye J.S., Curtis D.;
RT   "aph-1 and pen-2 are required for Notch pathway signaling, gamma-secretase
RT   cleavage of betaAPP, and presenilin protein accumulation.";
RL   Dev. Cell 3:85-97(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney marrow;
RX   PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA   Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA   Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA   Look A.T., Chen Z.;
RT   "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential subunit of the gamma-secretase complex, an
CC       endoprotease complex that catalyzes the intramembrane cleavage of
CC       integral proteins such as Notch receptors. It may represent a
CC       stabilizing cofactor for the presenilin homodimer that promotes the
CC       formation of a stable complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the gamma-secretase complex, a complex composed
CC       of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 and
CC       PEN2. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the APH-1 family. {ECO:0000305}.
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DR   EMBL; AF512428; AAM88325.1; -; mRNA.
DR   EMBL; AY423036; AAQ98012.1; -; mRNA.
DR   EMBL; BC071492; AAH71492.1; -; mRNA.
DR   RefSeq; NP_956409.1; NM_200115.1.
DR   AlphaFoldDB; Q8JHE9; -.
DR   SMR; Q8JHE9; -.
DR   STRING; 7955.ENSDARP00000011743; -.
DR   PaxDb; Q8JHE9; -.
DR   Ensembl; ENSDART00000008096; ENSDARP00000011743; ENSDARG00000005612.
DR   Ensembl; ENSDART00000191853; ENSDARP00000149060; ENSDARG00000111794.
DR   GeneID; 386808; -.
DR   KEGG; dre:386808; -.
DR   CTD; 83464; -.
DR   ZFIN; ZDB-GENE-031118-31; aph1b.
DR   eggNOG; KOG3972; Eukaryota.
DR   GeneTree; ENSGT00390000002049; -.
DR   HOGENOM; CLU_086389_0_0_1; -.
DR   InParanoid; Q8JHE9; -.
DR   OMA; EKKKWYV; -.
DR   OrthoDB; 1085102at2759; -.
DR   PhylomeDB; Q8JHE9; -.
DR   TreeFam; TF314362; -.
DR   PRO; PR:Q8JHE9; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 7.
DR   Bgee; ENSDARG00000005612; Expressed in mature ovarian follicle and 28 other tissues.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0070765; C:gamma-secretase complex; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IBA:GO_Central.
DR   GO; GO:0007220; P:Notch receptor processing; IBA:GO_Central.
DR   GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR   GO; GO:0043085; P:positive regulation of catalytic activity; IEA:InterPro.
DR   GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR   InterPro; IPR009294; Aph-1.
DR   PANTHER; PTHR12889; PTHR12889; 1.
DR   Pfam; PF06105; Aph-1; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Notch signaling pathway; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..258
FT                   /note="Gamma-secretase subunit Aph-1b"
FT                   /id="PRO_0000221056"
FT   TRANSMEM        3..23
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   258 AA;  28647 MW;  602EDCC9AD70A334 CRC64;
     MTVAVFFGCT FIAFGPAIAL FMFTIARDPL RVIFLIAGAF FWLVSLLLSS LVWFITVQIS
     NKNSATQQRG LLIFGVVLSV LLQEAFRYGY YRLLKKANEG LLALSQEDTM PISMRQLAYV
     SGLGFGFMSG AFSVVNILSD SLGPGTVGIH GESQHYFISS AFMTLAIILL HMFWGVVFFE
     ACERQRWWAL GAVVASHLVV SCLTFVNPHY QGSLIPTYII LSVMAVWAYL CAGGSLRNLK
     LCLTCKDKDF LLANHRPR