ID   IFNG1_CARAU             Reviewed;         182 AA.
AC   B5B3U4;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Interferon gamma 1 {ECO:0000305};
DE   AltName: Full=Interferon gamma {ECO:0000303|PubMed:18831986};
DE   AltName: Full=Interferon gamma 2 {ECO:0000303|PubMed:18831986};
DE   Flags: Precursor;
GN   Name=ifng1 {ECO:0000305};
OS   Carassius auratus (Goldfish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Cyprininae; Carassius.
OX   NCBI_TaxID=7957 {ECO:0000312|EMBL:ACG68885.1};
RN   [1] {ECO:0000312|EMBL:ACG68885.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND INDUCTION.
RX   PubMed=18831986; DOI=10.1016/j.dci.2008.09.001;
RA   Grayfer L., Belosevic M.;
RT   "Molecular characterization, expression and functional analysis of goldfish
RT   (Carassius auratus L.) interferon gamma.";
RL   Dev. Comp. Immunol. 33:235-246(2009).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   PubMed=19577303; DOI=10.1016/j.molimm.2009.06.004;
RA   Grayfer L., Belosevic M.;
RT   "Molecular characterization of novel interferon gamma receptor 1 isoforms
RT   in zebrafish (Danio rerio) and goldfish (Carassius auratus L.).";
RL   Mol. Immunol. 46:3050-3059(2009).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=20507977; DOI=10.1074/jbc.m109.096925;
RA   Grayfer L., Garcia E.G., Belosevic M.;
RT   "Comparison of macrophage antimicrobial responses induced by type II
RT   interferons of the goldfish (Carassius auratus L.).";
RL   J. Biol. Chem. 285:23537-23547(2010).
CC   -!- FUNCTION: Cytokine which binds to interferon gamma receptor 1-like
CC       (ifngr1l) (PubMed:18831986, PubMed:19577303, PubMed:20507977). Has
CC       activating effects on primary macrophages and neutrophils
CC       (PubMed:18831986, PubMed:20507977). Induces nitric oxide production and
CC       phagocytic responses in macrophages (PubMed:18831986, PubMed:20507977).
CC       Primes macrophages and neutrophils for production of reactive oxygen
CC       intermediates (ROI) (PubMed:18831986, PubMed:20507977). Stimulates
CC       phosphorylation and nuclear localization of the JAK/STAT signal
CC       transducer stat1 (PubMed:20507977). Promotes increased expression of a
CC       number of genes important for macrophage activity, including the
CC       interferon regulatory factors irf1, irf2, irf8 and irf9
CC       (PubMed:18831986, PubMed:20507977). {ECO:0000269|PubMed:18831986,
CC       ECO:0000269|PubMed:19577303, ECO:0000269|PubMed:20507977}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19577303}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:18831986,
CC       ECO:0000305|PubMed:19577303, ECO:0000305|PubMed:20507977}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in spleen. Also detected at lower
CC       levels in brain, gill, kidney, heart, intestine and muscle. In immune
CC       cell populations, has highest expression in peripheral blood leukocytes
CC       and splenocytes. Detected in kidney-derived monocytes, neutrophils,
CC       macrophages and leukocytes. {ECO:0000269|PubMed:18831986}.
CC   -!- INDUCTION: Strongly up-regulated in kidney leukocytes in response to
CC       phytohaemagglutinin (PHA). Also up-regulated to a lesser extent by
CC       polyinosinic:polycytidylic acid (poly(I:C)).
CC       {ECO:0000269|PubMed:18831986}.
CC   -!- SIMILARITY: Belongs to the type II (or gamma) interferon family.
CC       {ECO:0000305}.
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DR   EMBL; EU909368; ACG68885.1; -; mRNA.
DR   AlphaFoldDB; B5B3U4; -.
DR   SMR; B5B3U4; -.
DR   Proteomes; UP000515129; Genome assembly.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0005125; F:cytokine activity; IEA:UniProtKB-KW.
DR   GO; GO:0005133; F:interferon-gamma receptor binding; IEA:InterPro.
DR   GO; GO:0006955; P:immune response; IEA:InterPro.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR002069; Interferon_gamma.
DR   PANTHER; PTHR11419; PTHR11419; 1.
DR   Pfam; PF00714; IFN-gamma; 1.
DR   SUPFAM; SSF47266; SSF47266; 1.
PE   1: Evidence at protein level;
KW   Cytokine; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..182
FT                   /note="Interferon gamma 1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002830045"
FT   CARBOHYD        93
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   182 AA;  21517 MW;  4F67D3C8F4F446FE CRC64;
     MIAQNMTIFF WGVCLLTSGW ATYSEASVPE NLDKSIDELK AYYIKDDHEI HNAHPVFLRV
     LKDLKVNLEE PEQNLLMSII MDTYSRIFTR MENDSLDEAT KERIAHVQEH LKKLRENYFP
     GKSAELKTYA ETLWAIKEDD PVIQRKALFE LKRVYREATL LKNLKNKERR RRQAKNTKNL
     KS