APH1_ARATH
ID APH1_ARATH Reviewed; 250 AA.
AC Q8L9G7; Q8VZI0; Q9SIC5;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2003, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Gamma-secretase subunit APH1-like;
GN OrderedLocusNames=At2g31440; ORFNames=T28P16.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral proteins such as Notch receptors. {ECO:0000250}.
CC -!- SUBUNIT: Probable component of the gamma-secretase complex, a complex
CC composed of a presenilin homodimer, nicastrin, APH1 and PEN2.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the APH-1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007169; AAD26475.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08548.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62984.1; -; Genomic_DNA.
DR EMBL; AY064157; AAL36063.1; -; mRNA.
DR EMBL; AY097412; AAM19928.1; -; mRNA.
DR EMBL; AY088444; AAM65980.1; -; mRNA.
DR PIR; G84720; G84720.
DR RefSeq; NP_001325103.1; NM_001336332.1.
DR RefSeq; NP_565724.1; NM_128701.3.
DR AlphaFoldDB; Q8L9G7; -.
DR SMR; Q8L9G7; -.
DR BioGRID; 3049; 4.
DR IntAct; Q8L9G7; 2.
DR STRING; 3702.AT2G31440.1; -.
DR PaxDb; Q8L9G7; -.
DR PRIDE; Q8L9G7; -.
DR ProteomicsDB; 246914; -.
DR EnsemblPlants; AT2G31440.1; AT2G31440.1; AT2G31440.
DR EnsemblPlants; AT2G31440.2; AT2G31440.2; AT2G31440.
DR GeneID; 817702; -.
DR Gramene; AT2G31440.1; AT2G31440.1; AT2G31440.
DR Gramene; AT2G31440.2; AT2G31440.2; AT2G31440.
DR KEGG; ath:AT2G31440; -.
DR Araport; AT2G31440; -.
DR TAIR; locus:2061265; AT2G31440.
DR eggNOG; KOG3972; Eukaryota.
DR HOGENOM; CLU_1117281_0_0_1; -.
DR InParanoid; Q8L9G7; -.
DR OMA; FFNAFDT; -.
DR OrthoDB; 1085102at2759; -.
DR PhylomeDB; Q8L9G7; -.
DR PRO; PR:Q8L9G7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8L9G7; baseline and differential.
DR Genevisible; Q8L9G7; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0070765; C:gamma-secretase complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0043085; P:positive regulation of catalytic activity; IEA:InterPro.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR InterPro; IPR009294; Aph-1.
DR PANTHER; PTHR12889; PTHR12889; 1.
DR Pfam; PF06105; Aph-1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Notch signaling pathway; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..250
FT /note="Gamma-secretase subunit APH1-like"
FT /id="PRO_0000221059"
FT TRANSMEM 5..25
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT CONFLICT 69
FT /note="L -> V (in Ref. 4; AAM65980)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 27536 MW; D6CB4061C2C16D8E CRC64;
MTVAAGIGYA LVALGPSLSL FVSVISRKPF LILTVLSSTL LWLVSLIILS GLWRPFLPLK
ANVWWPYALL VITSVCFQEG LRFLFWKVYK RLEDVLDSFA DRISRPRLFL TDKLQIALAG
GLGHGVAHAV FFCLSLLTPA FGPATFYVER CSKVPFFLIS AIIALAFVTI HTFSMVIAFE
GYAKGNKVDQ IIVPVIHLTA GMLTLVNFAS EGCVIGVPLL YLVASLTLVH CGKMVWQRLL
ESRNQSSASR
