IFNG_MOUSE
ID IFNG_MOUSE Reviewed; 155 AA.
AC P01580; Q542B8;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Interferon gamma;
DE Short=IFN-gamma;
DE Flags: Precursor;
GN Name=Ifng;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=6310596; DOI=10.1073/pnas.80.19.5842;
RA Gray P.W., Goeddel D.V.;
RT "Cloning and expression of murine immune interferon cDNA.";
RL Proc. Natl. Acad. Sci. U.S.A. 80:5842-5846(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=8456301; DOI=10.1126/science.8456301;
RA Huang S., Hendriks W., Althage A., Hemmi S., Bluethmann H., Kamijo R.,
RA Vilcek J., Zinkernagel R.M., Aguet M.;
RT "Immune response in mice that lack the interferon-gamma receptor.";
RL Science 259:1742-1745(1993).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11585387; DOI=10.1006/cimm.2001.1819;
RA Pearl J.E., Saunders B., Ehlers S., Orme I.M., Cooper A.M.;
RT "Inflammation and lymphocyte activation during mycobacterial infection in
RT the interferon-gamma-deficient mouse.";
RL Cell. Immunol. 211:43-50(2001).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20535209; DOI=10.1038/nature09135;
RA Baldridge M.T., King K.Y., Boles N.C., Weksberg D.C., Goodell M.A.;
RT "Quiescent haematopoietic stem cells are activated by IFN-gamma in response
RT to chronic infection.";
RL Nature 465:793-797(2010).
RN [7]
RP FUNCTION.
RX PubMed=25078851; DOI=10.1002/stem.1799;
RA Matatall K.A., Shen C.C., Challen G.A., King K.Y.;
RT "Type II interferon promotes differentiation of myeloid-biased
RT hematopoietic stem cells.";
RL Stem Cells 32:3023-3030(2014).
CC -!- FUNCTION: Type II interferon produced by immune cells such as T-cells
CC and NK cells that plays crucial roles in antimicrobial, antiviral, and
CC antitumor responses by activating effector immune cells and enhancing
CC antigen presentation (PubMed:8456301, PubMed:11585387). Primarily
CC signals through the JAK-STAT pathway after interaction with its
CC receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1
CC intracellular domain opens out to allow association of downstream
CC signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation,
CC nuclear translocation and transcription of IFNG-regulated genes. Many
CC of the induced genes are transcription factors such as IRF1 that are
CC able to further drive regulation of a next wave of transcription. Plays
CC a role in class I antigen presentation pathway by inducing a
CC replacement of catalytic proteasome subunits with immunoproteasome
CC subunits. In turn, increases the quantity, quality, and repertoire of
CC peptides for class I MHC loading. Increases the efficiency of peptide
CC generation also by inducing the expression of activator PA28 that
CC associates with the proteasome and alters its proteolytic cleavage
CC preference. Up-regulates as well MHC II complexes on the cell surface
CC by promoting expression of several key molecules such as cathepsins
CC B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the
CC regulation of hematopoietic stem cells during development and under
CC homeostatic conditions by affecting their development, quiescence, and
CC differentiation (PubMed:20535209, PubMed:25078851).
CC {ECO:0000250|UniProtKB:P01579, ECO:0000269|PubMed:11585387,
CC ECO:0000269|PubMed:20535209, ECO:0000269|PubMed:25078851,
CC ECO:0000269|PubMed:8456301}.
CC -!- SUBUNIT: Homodimer. Interacts with IFNGR1 (via extracellular domain);
CC this interaction promotes IFNGR1 dimerization.
CC {ECO:0000250|UniProtKB:P01579}.
CC -!- INTERACTION:
CC P01580; P52293: Kpna2; NbExp=5; IntAct=EBI-7892102, EBI-3043908;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01579}.
CC -!- TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant mice show no special
CC developmental defects, and their immune system appear to develop
CC normally. However, they show susceptibility to bacterial or viral
CC infections such as vaccinia virus or Theiler's murine encephalomyelitis
CC virus, despite normal cytotoxic and T-helper cell responses.
CC {ECO:0000269|PubMed:11585387, ECO:0000269|PubMed:20535209,
CC ECO:0000269|PubMed:8456301}.
CC -!- SIMILARITY: Belongs to the type II (or gamma) interferon family.
CC {ECO:0000305}.
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DR EMBL; K00083; AAA37894.1; -; mRNA.
DR EMBL; AK089574; BAC40926.1; -; mRNA.
DR EMBL; BC119063; AAI19064.1; -; mRNA.
DR EMBL; BC119065; AAI19066.1; -; mRNA.
DR CCDS; CCDS24200.1; -.
DR PIR; A01844; IVMSG.
DR RefSeq; NP_032363.1; NM_008337.4.
DR AlphaFoldDB; P01580; -.
DR SMR; P01580; -.
DR IntAct; P01580; 3.
DR MINT; P01580; -.
DR STRING; 10090.ENSMUSP00000063800; -.
DR GlyGen; P01580; 2 sites.
DR PhosphoSitePlus; P01580; -.
DR EPD; P01580; -.
DR PaxDb; P01580; -.
DR PRIDE; P01580; -.
DR Antibodypedia; 4154; 3247 antibodies from 49 providers.
DR DNASU; 15978; -.
DR Ensembl; ENSMUST00000068592; ENSMUSP00000063800; ENSMUSG00000055170.
DR GeneID; 15978; -.
DR KEGG; mmu:15978; -.
DR UCSC; uc007hdy.1; mouse.
DR CTD; 3458; -.
DR MGI; MGI:107656; Ifng.
DR VEuPathDB; HostDB:ENSMUSG00000055170; -.
DR eggNOG; ENOG502SBGW; Eukaryota.
DR GeneTree; ENSGT00390000007831; -.
DR HOGENOM; CLU_135106_0_0_1; -.
DR InParanoid; P01580; -.
DR OMA; GGPIFTE; -.
DR OrthoDB; 1382686at2759; -.
DR PhylomeDB; P01580; -.
DR TreeFam; TF336308; -.
DR Reactome; R-MMU-877300; Interferon gamma signaling.
DR Reactome; R-MMU-877312; Regulation of IFNG signaling.
DR BioGRID-ORCS; 15978; 2 hits in 109 CRISPR screens.
DR PRO; PR:P01580; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; P01580; protein.
DR Bgee; ENSMUSG00000055170; Expressed in granulocyte and 10 other tissues.
DR Genevisible; P01580; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:CAFA.
DR GO; GO:0043005; C:neuron projection; ISO:MGI.
DR GO; GO:0043204; C:perikaryon; ISO:MGI.
DR GO; GO:0005125; F:cytokine activity; IDA:MGI.
DR GO; GO:0005133; F:interferon-gamma receptor binding; IEA:InterPro.
DR GO; GO:0002250; P:adaptive immune response; IDA:MGI.
DR GO; GO:0019882; P:antigen processing and presentation; IDA:MGI.
DR GO; GO:0006915; P:apoptotic process; ISO:MGI.
DR GO; GO:0048143; P:astrocyte activation; IGI:ARUK-UCL.
DR GO; GO:0002302; P:CD8-positive, alpha-beta T cell differentiation involved in immune response; IDA:MGI.
DR GO; GO:0071351; P:cellular response to interleukin-18; IDA:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IDA:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:MGI.
DR GO; GO:0042832; P:defense response to protozoan; IDA:MGI.
DR GO; GO:0051607; P:defense response to virus; IDA:MGI.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IDA:MGI.
DR GO; GO:0097191; P:extrinsic apoptotic signaling pathway; ISO:MGI.
DR GO; GO:0006959; P:humoral immune response; IDA:MGI.
DR GO; GO:0006925; P:inflammatory cell apoptotic process; IMP:MGI.
DR GO; GO:0060333; P:interferon-gamma-mediated signaling pathway; IDA:CAFA.
DR GO; GO:0002281; P:macrophage activation involved in immune response; ISO:MGI.
DR GO; GO:0030225; P:macrophage differentiation; ISO:MGI.
DR GO; GO:0001774; P:microglial cell activation; IGI:ARUK-UCL.
DR GO; GO:1900222; P:negative regulation of amyloid-beta clearance; IDA:ARUK-UCL.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0030857; P:negative regulation of epithelial cell differentiation; ISO:MGI.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; ISO:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:ARUK-UCL.
DR GO; GO:0072125; P:negative regulation of glomerular mesangial cell proliferation; ISO:MGI.
DR GO; GO:0032700; P:negative regulation of interleukin-17 production; ISO:MGI.
DR GO; GO:0031642; P:negative regulation of myelination; IDA:MGI.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; IDA:MGI.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:1902948; P:negative regulation of tau-protein kinase activity; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:CAFA.
DR GO; GO:0150076; P:neuroinflammatory response; ISO:MGI.
DR GO; GO:0001781; P:neutrophil apoptotic process; IMP:MGI.
DR GO; GO:0030593; P:neutrophil chemotaxis; IDA:MGI.
DR GO; GO:0006809; P:nitric oxide biosynthetic process; IDA:MGI.
DR GO; GO:0044794; P:positive regulation by host of viral process; IGI:ARUK-UCL.
DR GO; GO:1902004; P:positive regulation of amyloid-beta formation; IDA:ARUK-UCL.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISO:MGI.
DR GO; GO:0010508; P:positive regulation of autophagy; ISS:UniProtKB.
DR GO; GO:0060559; P:positive regulation of calcidiol 1-monooxygenase activity; ISO:MGI.
DR GO; GO:0032834; P:positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response; ISO:MGI.
DR GO; GO:0045785; P:positive regulation of cell adhesion; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:1901857; P:positive regulation of cellular respiration; IDA:ARUK-UCL.
DR GO; GO:0032722; P:positive regulation of chemokine production; IDA:MGI.
DR GO; GO:1904798; P:positive regulation of core promoter binding; ISO:MGI.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISO:MGI.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; ISO:MGI.
DR GO; GO:0060550; P:positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity; ISO:MGI.
DR GO; GO:0060552; P:positive regulation of fructose 1,6-bisphosphate metabolic process; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ARUK-UCL.
DR GO; GO:0045821; P:positive regulation of glycolytic process; IDA:ARUK-UCL.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISO:MGI.
DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; IDA:ARUK-UCL.
DR GO; GO:0032735; P:positive regulation of interleukin-12 production; IDA:MGI.
DR GO; GO:0032747; P:positive regulation of interleukin-23 production; ISO:MGI.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; IDA:MGI.
DR GO; GO:1904440; P:positive regulation of iron ion import across plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0048304; P:positive regulation of isotype switching to IgG isotypes; IDA:MGI.
DR GO; GO:0051712; P:positive regulation of killing of cells of another organism; ISO:MGI.
DR GO; GO:0060907; P:positive regulation of macrophage cytokine production; IDA:MGI.
DR GO; GO:0051044; P:positive regulation of membrane protein ectodomain proteolysis; ISO:MGI.
DR GO; GO:0045348; P:positive regulation of MHC class II biosynthetic process; IDA:BHF-UCL.
DR GO; GO:0050769; P:positive regulation of neurogenesis; ISO:MGI.
DR GO; GO:1901216; P:positive regulation of neuron death; IGI:ARUK-UCL.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISO:MGI.
DR GO; GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IDA:MGI.
DR GO; GO:0051770; P:positive regulation of nitric-oxide synthase biosynthetic process; IDA:ARUK-UCL.
DR GO; GO:0051173; P:positive regulation of nitrogen compound metabolic process; IGI:ARUK-UCL.
DR GO; GO:1904783; P:positive regulation of NMDA glutamate receptor activity; ISO:MGI.
DR GO; GO:0045672; P:positive regulation of osteoclast differentiation; ISO:MGI.
DR GO; GO:0033141; P:positive regulation of peptidyl-serine phosphorylation of STAT protein; ISO:MGI.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IDA:ARUK-UCL.
DR GO; GO:0090312; P:positive regulation of protein deacetylation; IDA:CAFA.
DR GO; GO:0042307; P:positive regulation of protein import into nucleus; ISO:MGI.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:MGI.
DR GO; GO:0071902; P:positive regulation of protein serine/threonine kinase activity; ISO:MGI.
DR GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISO:MGI.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; IGI:ARUK-UCL.
DR GO; GO:0034393; P:positive regulation of smooth muscle cell apoptotic process; ISO:MGI.
DR GO; GO:0032224; P:positive regulation of synaptic transmission, cholinergic; ISO:MGI.
DR GO; GO:0042102; P:positive regulation of T cell proliferation; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:2000309; P:positive regulation of tumor necrosis factor (ligand) superfamily member 11 production; ISO:MGI.
DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; IDA:BHF-UCL.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR GO; GO:0060557; P:positive regulation of vitamin D biosynthetic process; ISO:MGI.
DR GO; GO:0007259; P:receptor signaling pathway via JAK-STAT; IGI:ARUK-UCL.
DR GO; GO:0050691; P:regulation of defense response to virus by host; IDA:CAFA.
DR GO; GO:0060251; P:regulation of glial cell proliferation; ISO:MGI.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:2000345; P:regulation of hepatocyte proliferation; ISO:MGI.
DR GO; GO:0050776; P:regulation of immune response; IDA:MGI.
DR GO; GO:0050796; P:regulation of insulin secretion; ISO:MGI.
DR GO; GO:0098908; P:regulation of neuronal action potential; ISO:MGI.
DR GO; GO:0010835; P:regulation of protein ADP-ribosylation; ISO:MGI.
DR GO; GO:0002026; P:regulation of the force of heart contraction; ISO:MGI.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0009615; P:response to virus; ISO:MGI.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0050954; P:sensory perception of mechanical stimulus; ISO:MGI.
DR GO; GO:0042098; P:T cell proliferation; IDA:MGI.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IDA:MGI.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR002069; Interferon_gamma.
DR PANTHER; PTHR11419; PTHR11419; 1.
DR Pfam; PF00714; IFN-gamma; 1.
DR PIRSF; PIRSF001936; IFN-gamma; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Cytokine; Glycoprotein; Growth regulation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..155
FT /note="Interferon gamma"
FT /id="PRO_0000016453"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 155 AA; 17907 MW; 7EE80BAECB42071A CRC64;
MNATHCILAL QLFLMAVSGC YCHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD
GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE
VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC
