IFNG_RAT
ID IFNG_RAT Reviewed; 156 AA.
AC P01581;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Interferon gamma;
DE Short=IFN-gamma;
DE Flags: Precursor;
GN Name=Ifng;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3924594; DOI=10.1002/j.1460-2075.1985.tb03694.x;
RA Dijkema R., van der Meide P.H., Pouwels P.H., Caspers M., Dubbeld M.,
RA Schellekens H.;
RT "Cloning and expression of the chromosomal immune interferon gene of the
RT rat.";
RL EMBO J. 4:761-767(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3093809; DOI=10.1016/0076-6879(86)19065-3;
RA Dijkema R., der Meide P.H., Dubbeld M., Caspers M., Wubben J.,
RA Schellekens H.;
RT "Cloning, expression, and purification of rat IFN-gamma.";
RL Methods Enzymol. 119:453-463(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Fischer;
RA Widegren B., Visse E., Sjogren H.O.;
RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Type II interferon produced by immune cells such as T-cells
CC and NK cells that plays crucial roles in antimicrobial, antiviral, and
CC antitumor responses by activating effector immune cells and enhancing
CC antigen presentation. Primarily signals through the JAK-STAT pathway
CC after interaction with its receptor IFNGR1 to affect gene regulation.
CC Upon IFNG binding, IFNGR1 intracellular domain opens out to allow
CC association of downstream signaling components JAK2, JAK1 and STAT1,
CC leading to STAT1 activation, nuclear translocation and transcription of
CC IFNG-regulated genes. Many of the induced genes are transcription
CC factors such as IRF1 that are able to further drive regulation of a
CC next wave of transcription. Plays a role in class I antigen
CC presentation pathway by inducing a replacement of catalytic proteasome
CC subunits with immunoproteasome subunits. In turn, increases the
CC quantity, quality, and repertoire of peptides for class I MHC loading.
CC Increases the efficiency of peptide generation also by inducing the
CC expression of activator PA28 that associates with the proteasome and
CC alters its proteolytic cleavage preference. Up-regulates as well MHC II
CC complexes on the cell surface by promoting expression of several key
CC molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By
CC similarity). Participates in the regulation of hematopoietic stem cells
CC during development and under homeostatic conditions by affecting their
CC development, quiescence, and differentiation (By similarity).
CC {ECO:0000250|UniProtKB:P01579, ECO:0000250|UniProtKB:P01580}.
CC -!- SUBUNIT: Homodimer. Interacts with IFNGR1 (via extracellular domain);
CC this interaction promotes IFNGR1 dimerization.
CC {ECO:0000250|UniProtKB:P01579}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01579}.
CC -!- TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
CC -!- SIMILARITY: Belongs to the type II (or gamma) interferon family.
CC {ECO:0000305}.
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DR EMBL; X02325; CAA26185.1; -; Genomic_DNA.
DR EMBL; X02326; CAA26186.1; -; Genomic_DNA.
DR EMBL; X02326; CAA26187.1; -; Genomic_DNA.
DR EMBL; X02327; CAA26188.1; -; Genomic_DNA.
DR EMBL; M29317; AAA41362.1; -; Genomic_DNA.
DR EMBL; M29315; AAA41362.1; JOINED; Genomic_DNA.
DR EMBL; M29316; AAA41362.1; JOINED; Genomic_DNA.
DR EMBL; AF010466; AAB66352.1; -; mRNA.
DR PIR; A01845; IVRTG.
DR RefSeq; NP_620235.1; NM_138880.2.
DR AlphaFoldDB; P01581; -.
DR SMR; P01581; -.
DR STRING; 10116.ENSRNOP00000009917; -.
DR GlyGen; P01581; 2 sites.
DR PhosphoSitePlus; P01581; -.
DR PaxDb; P01581; -.
DR Ensembl; ENSRNOT00000009919; ENSRNOP00000009917; ENSRNOG00000007468.
DR GeneID; 25712; -.
DR KEGG; rno:25712; -.
DR UCSC; RGD:2866; rat.
DR CTD; 3458; -.
DR RGD; 2866; Ifng.
DR eggNOG; ENOG502SBGW; Eukaryota.
DR GeneTree; ENSGT00390000007831; -.
DR HOGENOM; CLU_135106_0_0_1; -.
DR InParanoid; P01581; -.
DR OMA; GGPIFTE; -.
DR OrthoDB; 1382686at2759; -.
DR PhylomeDB; P01581; -.
DR TreeFam; TF336308; -.
DR Reactome; R-RNO-877300; Interferon gamma signaling.
DR Reactome; R-RNO-877312; Regulation of IFNG signaling.
DR PRO; PR:P01581; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000007468; Expressed in thymus and 6 other tissues.
DR Genevisible; P01581; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0009897; C:external side of plasma membrane; ISO:RGD.
DR GO; GO:0005576; C:extracellular region; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0043005; C:neuron projection; IDA:RGD.
DR GO; GO:0043204; C:perikaryon; IDA:RGD.
DR GO; GO:0005125; F:cytokine activity; ISO:RGD.
DR GO; GO:0005133; F:interferon-gamma receptor binding; IEA:InterPro.
DR GO; GO:0002250; P:adaptive immune response; ISO:RGD.
DR GO; GO:0019882; P:antigen processing and presentation; ISO:RGD.
DR GO; GO:0006915; P:apoptotic process; ISO:RGD.
DR GO; GO:0048143; P:astrocyte activation; ISO:RGD.
DR GO; GO:0002302; P:CD8-positive, alpha-beta T cell differentiation involved in immune response; ISO:RGD.
DR GO; GO:0071351; P:cellular response to interleukin-18; ISO:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; ISO:RGD.
DR GO; GO:0042742; P:defense response to bacterium; ISO:RGD.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:RGD.
DR GO; GO:0042832; P:defense response to protozoan; ISO:RGD.
DR GO; GO:0051607; P:defense response to virus; ISO:RGD.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; ISO:RGD.
DR GO; GO:0097191; P:extrinsic apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0006959; P:humoral immune response; ISO:RGD.
DR GO; GO:0006925; P:inflammatory cell apoptotic process; ISO:RGD.
DR GO; GO:0060333; P:interferon-gamma-mediated signaling pathway; ISO:RGD.
DR GO; GO:0002281; P:macrophage activation involved in immune response; ISO:RGD.
DR GO; GO:0030225; P:macrophage differentiation; ISO:RGD.
DR GO; GO:0001774; P:microglial cell activation; IGI:ARUK-UCL.
DR GO; GO:1900222; P:negative regulation of amyloid-beta clearance; ISO:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0030857; P:negative regulation of epithelial cell differentiation; IDA:BHF-UCL.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; IDA:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:0072125; P:negative regulation of glomerular mesangial cell proliferation; IMP:RGD.
DR GO; GO:0032700; P:negative regulation of interleukin-17 production; ISO:RGD.
DR GO; GO:0031642; P:negative regulation of myelination; ISO:RGD.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; ISO:RGD.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:RGD.
DR GO; GO:1902948; P:negative regulation of tau-protein kinase activity; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0150076; P:neuroinflammatory response; IDA:ARUK-UCL.
DR GO; GO:0001781; P:neutrophil apoptotic process; ISO:RGD.
DR GO; GO:0030593; P:neutrophil chemotaxis; ISO:RGD.
DR GO; GO:0006809; P:nitric oxide biosynthetic process; IEA:Ensembl.
DR GO; GO:0044794; P:positive regulation by host of viral process; ISO:RGD.
DR GO; GO:1902004; P:positive regulation of amyloid-beta formation; ISO:RGD.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IDA:RGD.
DR GO; GO:0010508; P:positive regulation of autophagy; ISS:UniProtKB.
DR GO; GO:0060559; P:positive regulation of calcidiol 1-monooxygenase activity; ISO:RGD.
DR GO; GO:0032834; P:positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response; ISO:RGD.
DR GO; GO:0045785; P:positive regulation of cell adhesion; IDA:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:BHF-UCL.
DR GO; GO:1901857; P:positive regulation of cellular respiration; ISO:RGD.
DR GO; GO:0032722; P:positive regulation of chemokine production; ISO:RGD.
DR GO; GO:1904798; P:positive regulation of core promoter binding; ISO:RGD.
DR GO; GO:0001819; P:positive regulation of cytokine production; ISO:RGD.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; ISO:RGD.
DR GO; GO:0060550; P:positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity; ISO:RGD.
DR GO; GO:0060552; P:positive regulation of fructose 1,6-bisphosphate metabolic process; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ARUK-UCL.
DR GO; GO:0045821; P:positive regulation of glycolytic process; ISO:RGD.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISO:RGD.
DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; ISO:RGD.
DR GO; GO:0032735; P:positive regulation of interleukin-12 production; ISO:RGD.
DR GO; GO:0032747; P:positive regulation of interleukin-23 production; ISO:RGD.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISO:RGD.
DR GO; GO:1904440; P:positive regulation of iron ion import across plasma membrane; ISO:RGD.
DR GO; GO:0048304; P:positive regulation of isotype switching to IgG isotypes; ISO:RGD.
DR GO; GO:0051712; P:positive regulation of killing of cells of another organism; ISO:RGD.
DR GO; GO:0060907; P:positive regulation of macrophage cytokine production; IEA:Ensembl.
DR GO; GO:0051044; P:positive regulation of membrane protein ectodomain proteolysis; ISO:RGD.
DR GO; GO:0045348; P:positive regulation of MHC class II biosynthetic process; IEP:RGD.
DR GO; GO:0050769; P:positive regulation of neurogenesis; ISO:RGD.
DR GO; GO:1901216; P:positive regulation of neuron death; ISO:RGD.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; IDA:RGD.
DR GO; GO:0045429; P:positive regulation of nitric oxide biosynthetic process; ISO:RGD.
DR GO; GO:0051770; P:positive regulation of nitric-oxide synthase biosynthetic process; ISO:RGD.
DR GO; GO:0051173; P:positive regulation of nitrogen compound metabolic process; ISO:RGD.
DR GO; GO:1904783; P:positive regulation of NMDA glutamate receptor activity; IGI:ARUK-UCL.
DR GO; GO:0045672; P:positive regulation of osteoclast differentiation; ISO:RGD.
DR GO; GO:0033141; P:positive regulation of peptidyl-serine phosphorylation of STAT protein; IDA:BHF-UCL.
DR GO; GO:0050766; P:positive regulation of phagocytosis; ISO:RGD.
DR GO; GO:0090312; P:positive regulation of protein deacetylation; ISO:RGD.
DR GO; GO:0042307; P:positive regulation of protein import into nucleus; ISO:RGD.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:RGD.
DR GO; GO:0071902; P:positive regulation of protein serine/threonine kinase activity; ISO:RGD.
DR GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISO:RGD.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; ISO:RGD.
DR GO; GO:0034393; P:positive regulation of smooth muscle cell apoptotic process; ISO:RGD.
DR GO; GO:0032224; P:positive regulation of synaptic transmission, cholinergic; IDA:RGD.
DR GO; GO:0042102; P:positive regulation of T cell proliferation; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:2000309; P:positive regulation of tumor necrosis factor (ligand) superfamily member 11 production; ISO:RGD.
DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; ISO:RGD.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; IDA:BHF-UCL.
DR GO; GO:0060557; P:positive regulation of vitamin D biosynthetic process; ISO:RGD.
DR GO; GO:0007259; P:receptor signaling pathway via JAK-STAT; ISO:RGD.
DR GO; GO:0050691; P:regulation of defense response to virus by host; ISO:RGD.
DR GO; GO:0060251; P:regulation of glial cell proliferation; IDA:RGD.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:2000345; P:regulation of hepatocyte proliferation; IDA:RGD.
DR GO; GO:0050776; P:regulation of immune response; ISO:RGD.
DR GO; GO:0050796; P:regulation of insulin secretion; ISO:RGD.
DR GO; GO:0098908; P:regulation of neuronal action potential; IDA:RGD.
DR GO; GO:0010835; P:regulation of protein ADP-ribosylation; ISO:RGD.
DR GO; GO:0002026; P:regulation of the force of heart contraction; IDA:RGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0009615; P:response to virus; ISO:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0050954; P:sensory perception of mechanical stimulus; IDA:RGD.
DR GO; GO:0042098; P:T cell proliferation; IEA:Ensembl.
DR GO; GO:0050852; P:T cell receptor signaling pathway; ISO:RGD.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR002069; Interferon_gamma.
DR PANTHER; PTHR11419; PTHR11419; 1.
DR Pfam; PF00714; IFN-gamma; 1.
DR PIRSF; PIRSF001936; IFN-gamma; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
PE 2: Evidence at transcript level;
KW Antiviral defense; Cytokine; Glycoprotein; Growth regulation;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000250"
FT CHAIN 23..156
FT /note="Interferon gamma"
FT /id="PRO_0000016457"
FT MOD_RES 23
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:P01579"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 156 AA; 17918 MW; BC0B433D2CE1E3F2 CRC64;
MSATRRVLVL QLCLMALSGC YCQGTLIESL ESLKNYFNSS SMDAMEGKSL LLDIWRNWQK
DGNTKILESQ IISFYLRLFE VLKDNQAISN NISVIESHLI TNFFSNSKAK KDAFMSIAKF
EVNNPQIQHK AVNELIRVIH QLSPESSLRK RKRSRC
