IFNW1_HUMAN
ID IFNW1_HUMAN Reviewed; 195 AA.
AC P05000; Q13168; Q5U802; Q5VWD0; Q7M4P5;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 2.
DT 03-AUG-2022, entry version 189.
DE RecName: Full=Interferon omega-1;
DE AltName: Full=Interferon alpha-II-1;
DE Flags: Precursor;
GN Name=IFNW1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2985969; DOI=10.1128/mcb.5.4.768-779.1985;
RA Capon D.J., Shepard H.M., Goeddel D.V.;
RT "Two distinct families of human and bovine interferon-alpha genes are
RT coordinately expressed and encode functional polypeptides.";
RL Mol. Cell. Biol. 5:768-779(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leukocyte;
RX PubMed=1647209; DOI=10.1016/0167-4781(91)90004-6;
RA Adolf G.R., Fruehbeis B., Hauptmann R., Kalsner I., Maurer-Fogy I.,
RA Ostermann E., Patzelt E., Schwendenwein R., Sommergruber W., Zoephel A.;
RT "Human interferon omega 1: isolation of the gene, expression in Chinese
RT hamster ovary cells and characterization of the recombinant protein.";
RL Biochim. Biophys. Acta 1089:167-174(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 22-195.
RX PubMed=3895159; DOI=10.1093/nar/13.13.4739;
RA Hauptmann R., Swetly P.;
RT "A novel class of human type I interferons.";
RL Nucleic Acids Res. 13:4739-4749(1985).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-195.
RX PubMed=8142025;
RA Li M.F., Zeng Q., Zhou Y., Guo H.Y., Hou Y.D.;
RT "Cloning, sequencing and expression in E. coli of interferon-omega 1
RT gene.";
RL Sci. China, Ser. B, Chem. Life Sci. Earth Sci. 36:1361-1366(1993).
RN [8]
RP PROTEIN SEQUENCE OF 22-55.
RX PubMed=7765487; DOI=10.1271/bbb.58.1714;
RA Shirono H., Koga J., Uemura H., Matsuo A.;
RT "Identification of glycosylated subtypes of interferon-alpha produced by
RT human leukocytes.";
RL Biosci. Biotechnol. Biochem. 58:1714-1715(1994).
RN [9]
RP PROTEIN SEQUENCE OF 22-36.
RX PubMed=15340161; DOI=10.1110/ps.04682504;
RA Zhang Z., Henzel W.J.;
RT "Signal peptide prediction based on analysis of experimentally verified
RT cleavage sites.";
RL Protein Sci. 13:2819-2824(2004).
RN [10]
RP PROTEIN SEQUENCE OF 22-33, AND GLYCOSYLATION AT ASN-101.
RX PubMed=1693148; DOI=10.1016/s0021-9258(19)38846-5;
RA Adolf G.R., Maurer-Fogy I., Kalsner I., Cantell K.;
RT "Purification and characterization of natural human interferon omega 1. Two
RT alternative cleavage sites for the signal peptidase.";
RL J. Biol. Chem. 265:9290-9295(1990).
RN [11]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 24-195.
RC TISSUE=Liver;
RA Yang R.Y., Yang Z.F., Zhu Y.T., Feng L.L., Liu N., Zhao F., Zhang F.X.,
RA Fu L.C.;
RT "Human IFN-omega 1 gene isolated from embryonic hepar.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [12]
RP X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 22-195 IN COMPLEX WITH IFNAR1 AND
RP IFNAR2, AND DISULFIDE BOND.
RX PubMed=21854986; DOI=10.1016/j.cell.2011.06.048;
RA Thomas C., Moraga I., Levin D., Krutzik P.O., Podoplelova Y., Trejo A.,
RA Lee C., Yarden G., Vleck S.E., Glenn J.S., Nolan G.P., Piehler J.,
RA Schreiber G., Garcia K.C.;
RT "Structural linkage between ligand discrimination and receptor activation
RT by type I interferons.";
RL Cell 146:621-632(2011).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the alpha/beta interferon family. {ECO:0000305}.
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DR EMBL; M11003; AAA52724.1; -; Genomic_DNA.
DR EMBL; X58822; CAA41626.1; -; Genomic_DNA.
DR EMBL; AL390882; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471071; EAW58624.1; -; Genomic_DNA.
DR EMBL; BC069095; AAH69095.1; -; mRNA.
DR EMBL; BC117290; AAI17291.1; -; mRNA.
DR EMBL; BC117292; AAI17293.1; -; mRNA.
DR EMBL; X02669; CAA26501.1; -; mRNA.
DR EMBL; U25670; AAA70091.1; -; Genomic_DNA.
DR EMBL; AY780805; AAV49320.1; -; mRNA.
DR CCDS; CCDS6496.1; -.
DR PIR; A93070; IVHUII.
DR PIR; PC2204; PC2204.
DR PIR; PC2205; PC2205.
DR RefSeq; NP_002168.1; NM_002177.2.
DR PDB; 3SE4; X-ray; 3.50 A; B=22-195.
DR PDBsum; 3SE4; -.
DR AlphaFoldDB; P05000; -.
DR SMR; P05000; -.
DR BioGRID; 109689; 5.
DR ComplexPortal; CPX-6010; Interferon omega receptor-ligand complex.
DR CORUM; P05000; -.
DR IntAct; P05000; 3.
DR STRING; 9606.ENSP00000369578; -.
DR GlyConnect; 295; 2 N-Linked glycans (1 site).
DR GlyGen; P05000; 1 site, 4 N-linked glycans (1 site).
DR BioMuta; IFNW1; -.
DR DMDM; 400061; -.
DR MassIVE; P05000; -.
DR PaxDb; P05000; -.
DR PeptideAtlas; P05000; -.
DR PRIDE; P05000; -.
DR ProteomicsDB; 51761; -.
DR Antibodypedia; 24850; 131 antibodies from 21 providers.
DR DNASU; 3467; -.
DR Ensembl; ENST00000380229.4; ENSP00000369578.2; ENSG00000177047.7.
DR GeneID; 3467; -.
DR KEGG; hsa:3467; -.
DR MANE-Select; ENST00000380229.4; ENSP00000369578.2; NM_002177.3; NP_002168.1.
DR UCSC; uc003zol.2; human.
DR CTD; 3467; -.
DR DisGeNET; 3467; -.
DR GeneCards; IFNW1; -.
DR HGNC; HGNC:5448; IFNW1.
DR HPA; ENSG00000177047; Not detected.
DR MIM; 147553; gene.
DR neXtProt; NX_P05000; -.
DR OpenTargets; ENSG00000177047; -.
DR PharmGKB; PA29683; -.
DR VEuPathDB; HostDB:ENSG00000177047; -.
DR eggNOG; ENOG502T289; Eukaryota.
DR GeneTree; ENSGT01000000214430; -.
DR HOGENOM; CLU_109427_0_0_1; -.
DR InParanoid; P05000; -.
DR OMA; VVRMEIM; -.
DR OrthoDB; 1358010at2759; -.
DR PhylomeDB; P05000; -.
DR TreeFam; TF336177; -.
DR PathwayCommons; P05000; -.
DR SignaLink; P05000; -.
DR SIGNOR; P05000; -.
DR BioGRID-ORCS; 3467; 20 hits in 1061 CRISPR screens.
DR GeneWiki; IFNW1; -.
DR GenomeRNAi; 3467; -.
DR Pharos; P05000; Tbio.
DR PRO; PR:P05000; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; P05000; protein.
DR Bgee; ENSG00000177047; Expressed in developing anatomical structure and 1 other tissue.
DR Genevisible; P05000; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005126; F:cytokine receptor binding; TAS:ProtInc.
DR GO; GO:0005132; F:type I interferon receptor binding; IBA:GO_Central.
DR GO; GO:0002250; P:adaptive immune response; IBA:GO_Central.
DR GO; GO:0030183; P:B cell differentiation; IBA:GO_Central.
DR GO; GO:0042100; P:B cell proliferation; IBA:GO_Central.
DR GO; GO:0098586; P:cellular response to virus; IC:ComplexPortal.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0006959; P:humoral immune response; IBA:GO_Central.
DR GO; GO:0002323; P:natural killer cell activation involved in immune response; IBA:GO_Central.
DR GO; GO:0033141; P:positive regulation of peptidyl-serine phosphorylation of STAT protein; IBA:GO_Central.
DR GO; GO:0051726; P:regulation of cell cycle; TAS:ProtInc.
DR GO; GO:0043330; P:response to exogenous dsRNA; IBA:GO_Central.
DR GO; GO:0009615; P:response to virus; TAS:ProtInc.
DR GO; GO:0002286; P:T cell activation involved in immune response; IBA:GO_Central.
DR GO; GO:0060337; P:type I interferon signaling pathway; IC:ComplexPortal.
DR CDD; cd00095; IFab; 1.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000471; Interferon_alpha/beta/delta.
DR PANTHER; PTHR11691; PTHR11691; 1.
DR Pfam; PF00143; Interferon; 1.
DR PRINTS; PR00266; INTERFERONAB.
DR SMART; SM00076; IFabd; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00252; INTERFERON_A_B_D; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; Cytokine; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /note="Or 23 in some molecules"
FT /evidence="ECO:0000269|PubMed:15340161,
FT ECO:0000269|PubMed:1693148, ECO:0000269|PubMed:7765487"
FT CHAIN 22..195
FT /note="Interferon omega-1"
FT /id="PRO_0000016408"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:1693148"
FT /id="CAR_000050"
FT DISULFID 24..122
FT /evidence="ECO:0000250"
FT DISULFID 52..162
FT /evidence="ECO:0000269|PubMed:21854986"
FT VARIANT 95
FT /note="R -> S (in dbSNP:rs2230055)"
FT /id="VAR_020028"
FT CONFLICT 22..23
FT /note="LG -> EF (in Ref. 7; AAA70091)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="H -> M (in Ref. 8; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="V -> A (in Ref. 7; AAA70091)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="G -> E (in Ref. 1; AAA52724)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="V -> D (in Ref. 7; AAA70091)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 22319 MW; 1E4306F3487987FA CRC64;
MALLFPLLAA LVMTSYSPVG SLGCDLPQNH GLLSRNTLVL LHQMRRISPF LCLKDRRDFR
FPQEMVKGSQ LQKAHVMSVL HEMLQQIFSL FHTERSSAAW NMTLLDQLHT GLHQQLQHLE
TCLLQVVGEG ESAGAISSPA LTLRRYFQGI RVYLKEKKYS DCAWEVVRME IMKSLFLSTN
MQERLRSKDR DLGSS
