IFRH_ARATH
ID IFRH_ARATH Reviewed; 310 AA.
AC P52577; Q9FRL9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Isoflavone reductase homolog P3;
DE EC=1.3.1.-;
GN OrderedLocusNames=At1g75280; ORFNames=F22H5.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=7592828; DOI=10.1074/jbc.270.44.26224;
RA Babiychuk E., Kushnir S., Belles-Boix E., van Montagu M., Inze D.;
RT "Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of
RT yeasts toward the thiol-oxidizing drug diamide.";
RL J. Biol. Chem. 270:26224-26231(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY CADMIUM.
RC STRAIN=cv. Columbia;
RX PubMed=16502469; DOI=10.1002/pmic.200500543;
RA Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V.,
RA Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C.,
RA Ezan E., Garin J., Bourguignon J.;
RT "The early responses of Arabidopsis thaliana cells to cadmium exposure
RT explored by protein and metabolite profiling analyses.";
RL Proteomics 6:2180-2198(2006).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Induced by cadmium. {ECO:0000269|PubMed:16502469}.
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone
CC reductase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG12677.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z49777; CAA89859.1; -; mRNA.
DR EMBL; AC025814; AAG12677.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35697.1; -; Genomic_DNA.
DR EMBL; AY065395; AAL38836.1; -; mRNA.
DR EMBL; AY117175; AAM51250.1; -; mRNA.
DR EMBL; AY084851; AAM61416.1; -; mRNA.
DR PIR; C96783; C96783.
DR PIR; S57613; S57613.
DR RefSeq; NP_565107.1; NM_106183.4.
DR AlphaFoldDB; P52577; -.
DR SMR; P52577; -.
DR BioGRID; 29084; 1.
DR STRING; 3702.AT1G75280.1; -.
DR iPTMnet; P52577; -.
DR PaxDb; P52577; -.
DR PRIDE; P52577; -.
DR ProteomicsDB; 228737; -.
DR EnsemblPlants; AT1G75280.1; AT1G75280.1; AT1G75280.
DR GeneID; 843865; -.
DR Gramene; AT1G75280.1; AT1G75280.1; AT1G75280.
DR KEGG; ath:AT1G75280; -.
DR Araport; AT1G75280; -.
DR TAIR; locus:2025192; AT1G75280.
DR eggNOG; ENOG502QPMY; Eukaryota.
DR HOGENOM; CLU_060833_0_1_1; -.
DR InParanoid; P52577; -.
DR OMA; IIDDERT; -.
DR OrthoDB; 936727at2759; -.
DR PhylomeDB; P52577; -.
DR PRO; PR:P52577; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P52577; baseline and differential.
DR Genevisible; P52577; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR CDD; cd05259; PCBER_SDR_a; 1.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR008030; NmrA-like.
DR InterPro; IPR045312; PCBER-like.
DR Pfam; PF05368; NmrA; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..310
FT /note="Isoflavone reductase homolog P3"
FT /id="PRO_0000204548"
FT ACT_SITE 134
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 12..18
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 37
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 46
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 138
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
SQ SEQUENCE 310 AA; 33737 MW; B6D3F3D977176AC1 CRC64;
MATEKSKILV IGGTGYIGKF LVEASAKAGH STFALVREAT LSDPVKGKTV QSFKDLGVTI
LHGDLNDHES LVKAIKQVDV VISTVGSMQI LDQTKIISAI KEAGNVKRFL PSEFGVDVDR
TSAVEPAKSA FAGKIQIRRT IEAEGIPYTY AVTGCFGGYY LPTLVQFEPG LTSPPRDKVT
ILGDGNAKAV INKEEDIAAY TIKAVDDPRT LNKILYIKPS NNTLSMNEIV TLWEKKIGKS
LEKTHLPEEQ LLKSIQESPI PINVVLSINH AVFVNGDTNI SIEPSFGVEA SELYPDVKYT
SVDEYLSYFA
