IFRH_LUPAL
ID IFRH_LUPAL Reviewed; 312 AA.
AC P52581;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Isoflavone reductase homolog;
DE EC=1.3.1.-;
OS Lupinus albus (White lupine) (Lupinus termis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC genistoids sensu lato; core genistoids; Genisteae; Lupinus.
OX NCBI_TaxID=3870;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Primovski; TISSUE=Root;
RA Attucci S., Aitken S.M., Ibrahim R.K., Gulick P.J.;
RT "A cDNA clone encoding an isoflavone reductase-like protein from white
RT lupin.";
RL (er) Plant Gene Register PGR96-012(1996).
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone
CC reductase subfamily. {ECO:0000305}.
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DR EMBL; U48590; AAB67729.1; -; mRNA.
DR PIR; T11035; T11035.
DR AlphaFoldDB; P52581; -.
DR SMR; P52581; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0009807; P:lignan biosynthetic process; IEA:UniProt.
DR CDD; cd05259; PCBER_SDR_a; 1.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR008030; NmrA-like.
DR InterPro; IPR045312; PCBER-like.
DR Pfam; PF05368; NmrA; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW NADP; Oxidoreductase.
FT CHAIN 1..312
FT /note="Isoflavone reductase homolog"
FT /id="PRO_0000204552"
FT ACT_SITE 138
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 10..16
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 35
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 44
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 142
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT BINDING 270
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9LD14"
SQ SEQUENCE 312 AA; 35129 MW; 6355CCB341411218 CRC64;
MGKSKVLVVG GTGYVGRRIV KASLEHGHET FILQRPEIGL DIEKLQILLS FKKQGAILVE
ASFSDHKSLV DAVKLVDVVI CTMSGVHFRS HNLLTQLKLV EAIKDAGNIK RFLPSEFGMD
PALMGHALEP GRVTFDEKMT VRKAIEEANI PFTYISANCF AGYFAGNLSQ MKTLLPPRDK
VLLYGDGNVK PVYMDEDDVA TYTIKTIDDP RTLNKTVYLR PPENILTHKE LIEKWEELIG
KQLEKNSISE KDFLSTLKGL DFASQVGVGH FYHIFYEGCL TNFEIGENGE EASELYPEVN
YTRMDQYLKV YV