ID   IFRH_SOLTU              Reviewed;         308 AA.
AC   P52578;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Isoflavone reductase homolog;
DE            EC=1.3.1.-;
DE   AltName: Full=CP100;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9106515; DOI=10.1023/a:1005749913203;
RA   van Eldik G.J., Ruiter R.K., Colla P.H., van Herpen M.M.A.,
RA   Schrauwen J.A.M., Wullems G.J.;
RT   "Expression of an isoflavone reductase-like gene enhanced by pollen tube
RT   growth in pistils of Solanum tuberosum.";
RL   Plant Mol. Biol. 33:923-929(1997).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone
CC       reductase subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X92075; CAA63056.1; -; mRNA.
DR   PIR; T07386; T07386.
DR   RefSeq; NP_001274923.1; NM_001287994.1.
DR   AlphaFoldDB; P52578; -.
DR   SMR; P52578; -.
DR   STRING; 4113.PGSC0003DMT400084271; -.
DR   GeneID; 102586164; -.
DR   KEGG; sot:102586164; -.
DR   eggNOG; ENOG502QPMY; Eukaryota.
DR   OrthoDB; 936727at2759; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P52578; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   CDD; cd05259; PCBER_SDR_a; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR008030; NmrA-like.
DR   InterPro; IPR045312; PCBER-like.
DR   Pfam; PF05368; NmrA; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..308
FT                   /note="Isoflavone reductase homolog"
FT                   /id="PRO_0000204550"
FT   ACT_SITE        133
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         11..17
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         36
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         45
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
FT   BINDING         137
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q9LD14"
SQ   SEQUENCE   308 AA;  33852 MW;  1C68E610CA6C26A6 CRC64;
     MAGKSKILFI GGTGYIGKFI VEASAKAGHD TFVLVRESTL SNPTKTKLID TFKSFGVTFV
     HGDLYDHESL VKAIKQVDVV ISTVGHALLA DQVKLIAAIK EAGNVKRFFP SEFGNDVDRV
     HAVEPAKAAF NTKAQIRRVV EAEGIPFTYV ATFFFAGYSL PNLAQPGAAG PPNDKVVILG
     HGNTKAVFNK EEDIGTYTIN AVDDPKTLNK ILYIKPPHNI ITLNELVSLW EKKTGKNLER
     LYVPEEQVLK NIQEASVPMN VGLSIYHTAF VKGDHTNFEI EPSFGVEASE VYPDVKYTPI
     DEILNQYV