IFT20_BOVIN
ID IFT20_BOVIN Reviewed; 156 AA.
AC Q58CS6;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Intraflagellar transport protein 20 homolog;
GN Name=IFT20;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Hypothalamus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11916979; DOI=10.1083/jcb.200107108;
RA Pazour G.J., Baker S.A., Deane J.A., Cole D.G., Dickert B.L.,
RA Rosenbaum J.L., Witman G.B., Besharse J.C.;
RT "The intraflagellar transport protein, IFT88, is essential for vertebrate
RT photoreceptor assembly and maintenance.";
RL J. Cell Biol. 157:103-113(2002).
CC -!- FUNCTION: Part of intraflagellar transport (IFT) particles involved in
CC ciliary process assembly. May play a role in the trafficking of ciliary
CC membrane proteins from the Golgi complex to the cilium. Regulates the
CC platelet-derived growth factor receptor-alpha (PDGFRA) signaling
CC pathway. Required for protein stability of E3 ubiquitin ligases CBL and
CC CBLB that mediate ubiquitination and internalization of PDGFRA for
CC proper feedback inhibition of PDGFRA signaling. Essential for male
CC fertility. Plays an important role in spermatogenesis, particularly
CC spermiogenesis, when germ cells form flagella. May play a role in the
CC transport of flagellar proteins ODF2 and SPAG16 to build sperm flagella
CC and in the removal of redundant sperm cytoplasm. Also involved in
CC autophagy since it is required for trafficking of ATG16L and the
CC expansion of the autophagic compartment.
CC {ECO:0000250|UniProtKB:Q61025}.
CC -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74,
CC IFT80, IFT81, and IFT88. Interacts directly with IFT57 and
CC KIF3B/Kinesin II subunit. Interacts with IFT88 (By similarity).
CC Interacts with CEP83 (By similarity). Interacts with SPEF2 (via C-
CC terminus) (By similarity). Interacts with CBL and CBLB (By similarity).
CC Interacts with TRIP11 (By similarity). Interacts with TTC21A (By
CC similarity). Interacts with SPATA1 (By similarity). Interacts with
CC USH1G (By similarity). {ECO:0000250|UniProtKB:Q61025,
CC ECO:0000250|UniProtKB:Q8IY31}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network
CC {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriole
CC {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000250|UniProtKB:Q61025}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q61025}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q61025}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q61025}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:Q61025}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q61025}. Note=Present at the centrosomes during
CC the cell cycle and associated with the proximal portion of the mother
CC centriole and the lateral aspect of the daughter centriole. Associated
CC with basal body at the base of primary cilia. Detected in the Golgi
CC apparatus of round spermatids and late spermatocytes. Also detected in
CC the manchette of step 10-12 spermatids. In step 14 spermatids, found in
CC the basal body of the sperm tail. Localization in the manchette of
CC elongating spermatids is dependent on SPAG17.
CC {ECO:0000250|UniProtKB:Q61025}.
CC -!- TISSUE SPECIFICITY: Expressed in retina and are more abundant in the
CC inner segment of photoreceptors. {ECO:0000269|PubMed:11916979}.
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DR EMBL; BT021871; AAX46718.1; -; mRNA.
DR EMBL; BC103453; AAI03454.1; -; mRNA.
DR RefSeq; NP_001013621.1; NM_001013603.1.
DR RefSeq; XP_005220127.1; XM_005220070.3.
DR RefSeq; XP_005220128.1; XM_005220071.3.
DR AlphaFoldDB; Q58CS6; -.
DR SMR; Q58CS6; -.
DR STRING; 9913.ENSBTAP00000010668; -.
DR PaxDb; Q58CS6; -.
DR PRIDE; Q58CS6; -.
DR Ensembl; ENSBTAT00000010668; ENSBTAP00000010668; ENSBTAG00000008110.
DR Ensembl; ENSBTAT00000084852; ENSBTAP00000056882; ENSBTAG00000008110.
DR GeneID; 445364; -.
DR KEGG; bta:445364; -.
DR CTD; 90410; -.
DR VEuPathDB; HostDB:ENSBTAG00000008110; -.
DR VGNC; VGNC:50160; IFT20.
DR eggNOG; ENOG502RYYR; Eukaryota.
DR GeneTree; ENSGT00390000003413; -.
DR HOGENOM; CLU_134163_2_0_1; -.
DR InParanoid; Q58CS6; -.
DR OMA; TMAKQRQ; -.
DR OrthoDB; 1589590at2759; -.
DR TreeFam; TF319434; -.
DR Reactome; R-BTA-5620924; Intraflagellar transport.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000008110; Expressed in adenohypophysis and 107 other tissues.
DR GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0097546; C:ciliary base; ISS:UniProtKB.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0005801; C:cis-Golgi network; IEA:Ensembl.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0044292; C:dendrite terminus; IEA:Ensembl.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:1902636; C:kinociliary basal body; IEA:Ensembl.
DR GO; GO:0002177; C:manchette; ISS:UniProtKB.
DR GO; GO:0005902; C:microvillus; IEA:Ensembl.
DR GO; GO:0031514; C:motile cilium; IEA:Ensembl.
DR GO; GO:0032391; C:photoreceptor connecting cilium; ISS:UniProtKB.
DR GO; GO:0001750; C:photoreceptor outer segment; IEA:Ensembl.
DR GO; GO:0032420; C:stereocilium; IEA:Ensembl.
DR GO; GO:0002046; F:opsin binding; IEA:Ensembl.
DR GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; IEA:Ensembl.
DR GO; GO:0051642; P:centrosome localization; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; IEA:Ensembl.
DR GO; GO:0090102; P:cochlea development; IEA:Ensembl.
DR GO; GO:0045198; P:establishment of epithelial cell apical/basal polarity; IEA:Ensembl.
DR GO; GO:0001736; P:establishment of planar polarity; IEA:Ensembl.
DR GO; GO:0060122; P:inner ear receptor cell stereocilium organization; IEA:Ensembl.
DR GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR GO; GO:0001822; P:kidney development; IEA:Ensembl.
DR GO; GO:0061351; P:neural precursor cell proliferation; IEA:Ensembl.
DR GO; GO:0036372; P:opsin transport; IEA:Ensembl.
DR GO; GO:0035845; P:photoreceptor cell outer segment organization; IEA:Ensembl.
DR GO; GO:0045724; P:positive regulation of cilium assembly; IEA:Ensembl.
DR GO; GO:0061512; P:protein localization to cilium; IBA:GO_Central.
DR GO; GO:0034067; P:protein localization to Golgi apparatus; IEA:Ensembl.
DR GO; GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl.
DR GO; GO:2000785; P:regulation of autophagosome assembly; ISS:UniProtKB.
DR GO; GO:0060828; P:regulation of canonical Wnt signaling pathway; IEA:Ensembl.
DR GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB.
DR GO; GO:2000583; P:regulation of platelet-derived growth factor receptor-alpha signaling pathway; ISS:UniProtKB.
DR GO; GO:0007224; P:smoothened signaling pathway; IEA:Ensembl.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0072089; P:stem cell proliferation; IEA:Ensembl.
DR GO; GO:0008542; P:visual learning; IEA:Ensembl.
DR InterPro; IPR028172; FT20.
DR PANTHER; PTHR31978; PTHR31978; 1.
DR Pfam; PF14931; IFT20; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Differentiation;
KW Golgi apparatus; Reference proteome; Spermatogenesis.
FT CHAIN 1..156
FT /note="Intraflagellar transport protein 20 homolog"
FT /id="PRO_0000249302"
FT REGION 94..156
FT /note="IFT57-binding"
FT /evidence="ECO:0000250"
FT COILED 98..138
FT /evidence="ECO:0000255"
SQ SEQUENCE 156 AA; 17847 MW; 1F7DFC428A95DDF6 CRC64;
MTHLSLADPV REPLFSGEAG RQTAMAKDIL AEAGLHFDEL NKLRVLDPEV SQQTIELKEE
CKDFVDKIGQ FQKIVGGLIE LVDQLAKEAE NEKMKAIGAR NLLKSIAKQR EAQQQQLQAL
IAEKKMQLER YRVEYEALCK VEAEQNEFID QFIFQK
