IFT22_TRYB2
ID IFT22_TRYB2 Reviewed; 219 AA.
AC Q381A3;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Intraflagellar transport protein 22;
GN Name=IFT22; ORFNames=Tb11.01.8590;
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19240117; DOI=10.1242/jcs.040444;
RA Adhiambo C., Blisnick T., Toutirais G., Delannoy E., Bastin P.;
RT "A novel function for the atypical small G protein Rab-like 5 in the
RT assembly of the trypanosome flagellum.";
RL J. Cell Sci. 122:834-841(2009).
CC -!- FUNCTION: Required for flagellum formation.
CC {ECO:0000269|PubMed:19240117}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum basal body
CC {ECO:0000269|PubMed:19240117}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:19240117}. Note=ColocaliZes with intraflagellar
CC transport (IFT) proteins at the basal body and in the flagellum matrix.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; CH464491; EAN80628.1; -; Genomic_DNA.
DR RefSeq; XP_829740.1; XM_824647.1.
DR PDB; 6IA7; X-ray; 2.30 A; A/B=1-219.
DR PDB; 6IAE; X-ray; 2.49 A; A/B=1-219.
DR PDB; 6IAN; X-ray; 3.20 A; E=1-219.
DR PDBsum; 6IA7; -.
DR PDBsum; 6IAE; -.
DR PDBsum; 6IAN; -.
DR AlphaFoldDB; Q381A3; -.
DR SMR; Q381A3; -.
DR STRING; 5691.EAN80628; -.
DR PaxDb; Q381A3; -.
DR GeneID; 3664573; -.
DR KEGG; tbr:Tb11.01.8590; -.
DR VEuPathDB; TriTrypDB:Tb927.11.16830; -.
DR eggNOG; ENOG502RXD4; Eukaryota.
DR InParanoid; Q381A3; -.
DR Proteomes; UP000008524; Chromosome 11 Scaffold 1.
DR GO; GO:0036064; C:ciliary basal body; IDA:GeneDB.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0030992; C:intraciliary transport particle B; IPI:GeneDB.
DR GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW GTP-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..219
FT /note="Intraflagellar transport protein 22"
FT /id="PRO_0000429421"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 72..79
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:6IA7"
FT HELIX 18..25
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 41..47
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 69..74
FT /evidence="ECO:0007829|PDB:6IA7"
FT TURN 80..83
FT /evidence="ECO:0007829|PDB:6IAN"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 93..98
FT /evidence="ECO:0007829|PDB:6IA7"
FT HELIX 105..116
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 145..147
FT /evidence="ECO:0007829|PDB:6IAN"
FT STRAND 149..151
FT /evidence="ECO:0007829|PDB:6IA7"
FT TURN 162..166
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 169..172
FT /evidence="ECO:0007829|PDB:6IA7"
FT STRAND 174..177
FT /evidence="ECO:0007829|PDB:6IA7"
FT HELIX 181..198
FT /evidence="ECO:0007829|PDB:6IA7"
SQ SEQUENCE 219 AA; 24467 MW; 849EE097AF5E8103 CRC64;
MSDDLVKILV LGPSKSGKST VTNFLAGTRD TPTKEYHETN PLRVLEVEIA LDDTRRSGRQ
AAGLKKAVVQ LWDVGGSSKH QAGWPAIASN ADGIIYVFNP EVKGSEKELL LWYKNFALNQ
DELDDDNNFK MRVTDGHSLI FSHHSSLPEF AVGDNAIPPM PKQLQGIRAL ETSLDYQSDN
FKEAFDALVE QIIASRLAAE ENDLLQKERE AKDYPRLKR
