IFT27_BOVIN
ID IFT27_BOVIN Reviewed; 186 AA.
AC Q0VCN3; A6H6X3;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Intraflagellar transport protein 27 homolog;
DE Short=IFT27;
DE AltName: Full=Putative GTP-binding protein RAY-like;
DE AltName: Full=Rab-like protein 4;
GN Name=IFT27; Synonyms=RABL4, RAYL;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal brain, and Fetal cerebellum;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Small GTPase-like component of the intraflagellar transport
CC (IFT) complex B that promotes the exit of the BBSome complex from cilia
CC via its interaction with ARL6. Not involved in entry of the BBSome
CC complex into cilium. Prevents aggregation of GTP-free ARL6. Required
CC for hedgehog signaling. Forms a subcomplex within the IFT complex B
CC with IFT25. Its role in intraflagellar transport is mainly seen in
CC tissues rich in ciliated cells such as kidney and testis. Essential for
CC male fertility, spermiogenesis and sperm flagella formation. Plays a
CC role in the early development of the kidney. May be involved in the
CC regulation of ureteric bud initiation. {ECO:0000250|UniProtKB:A8HN58,
CC ECO:0000250|UniProtKB:Q9D0P8}.
CC -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74,
CC IFT80, IFT81, and IFT88. Interacts with HSPB11/IFT25. Interacts with
CC TTC30B (By similarity). Interacts with RABL2/RABL2A; binding is equal
CC in the presence of GTP or GDP. Interacts with IFT88. Interacts with
CC ARL6; recognizes and binds with the GTP-free form of ARL6.
CC {ECO:0000250|UniProtKB:A8HN58, ECO:0000250|UniProtKB:Q9D0P8}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q9D0P8}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q9D0P8}. Cell projection, cilium, flagellum
CC {ECO:0000250|UniProtKB:Q9D0P8}. Note=Localizes to the sperm flagellum.
CC {ECO:0000250|UniProtKB:Q9D0P8}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; BC120086; AAI20087.1; -; mRNA.
DR EMBL; BC146031; AAI46032.1; -; mRNA.
DR RefSeq; NP_001069913.1; NM_001076445.1.
DR AlphaFoldDB; Q0VCN3; -.
DR SMR; Q0VCN3; -.
DR STRING; 9913.ENSBTAP00000037810; -.
DR PaxDb; Q0VCN3; -.
DR Ensembl; ENSBTAT00000037991; ENSBTAP00000037810; ENSBTAG00000026657.
DR GeneID; 617147; -.
DR KEGG; bta:617147; -.
DR CTD; 11020; -.
DR VEuPathDB; HostDB:ENSBTAG00000026657; -.
DR eggNOG; KOG0079; Eukaryota.
DR GeneTree; ENSGT00870000136549; -.
DR HOGENOM; CLU_041217_10_6_1; -.
DR InParanoid; Q0VCN3; -.
DR OMA; EMSVKEM; -.
DR OrthoDB; 1585129at2759; -.
DR TreeFam; TF329292; -.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000026657; Expressed in olfactory segment of nasal mucosa and 106 other tissues.
DR ExpressionAtlas; Q0VCN3; baseline and differential.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
DR GO; GO:0042073; P:intraciliary transport; ISS:UniProtKB.
DR GO; GO:0001822; P:kidney development; ISS:UniProtKB.
DR GO; GO:0007224; P:smoothened signaling pathway; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd04101; RabL4; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR034112; RabL4_euk.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cytoplasm; Differentiation; Flagellum;
KW GTP-binding; Nucleotide-binding; Protein transport; Reference proteome;
KW Spermatogenesis; Transport.
FT CHAIN 1..186
FT /note="Intraflagellar transport protein 27 homolog"
FT /id="PRO_0000283072"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 64..68
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 123..126
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 186 AA; 20646 MW; B4B999C8E3A548EE CRC64;
MVKLAAKCIL AGDPTVGKTA LAQLFRSDGA HFQKNYTLTT GVDLVVKTVP VPDTGDSVEL
FIFDSAGKEL FSEMLDKLWE SPNVLCLVYD VTNEQSFTNC SKWLEKARSQ IPGTTLPGVL
VGNKTDLAGR RVVDVAQAQA WALGQGLECF ETSVKEMENY EAPFHYLAKQ FHHLYREKLE
VFQALV
