IFT27_TRYB2
ID IFT27_TRYB2 Reviewed; 183 AA.
AC Q580S0; D6XEA9;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Intraflagellar transport protein 27 homolog;
GN ORFNames=Tb927.3.5550 {ECO:0000312|EMBL:AAX81064.1};
OS Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX NCBI_TaxID=185431;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX PubMed=16020726; DOI=10.1126/science.1112642;
RA Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT "The genome of the African trypanosome Trypanosoma brucei.";
RL Science 309:416-422(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP THR-19 AND GLN-67.
RX PubMed=24843028; DOI=10.7554/elife.02419;
RA Huet D., Blisnick T., Perrot S., Bastin P.;
RT "The GTPase IFT27 is involved in both anterograde and retrograde
RT intraflagellar transport.";
RL Elife 3:E02419-E02419(2014).
CC -!- FUNCTION: Small GTPase-like component of the intraflagellar transport
CC (IFT) complex B required for both anterograde and retrograde
CC intraflagellar transport. May be involved in cargo loading of the
CC retrograde transport. {ECO:0000269|PubMed:24843028}.
CC -!- SUBUNIT: Component of the IFT complex B.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:24843028}.
CC -!- DEVELOPMENTAL STAGE: Expressed during procyclic stages.
CC {ECO:0000269|PubMed:24843028}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; AC093543; AAX81064.1; -; Genomic_DNA.
DR EMBL; CP000066; AAZ10586.1; -; Genomic_DNA.
DR RefSeq; XP_844145.1; XM_839052.1.
DR AlphaFoldDB; Q580S0; -.
DR SMR; Q580S0; -.
DR STRING; 5691.AAZ10586; -.
DR PaxDb; Q580S0; -.
DR GeneID; 3656495; -.
DR KEGG; tbr:Tb927.3.5550; -.
DR VEuPathDB; TriTrypDB:Tb927.3.5550; -.
DR eggNOG; KOG0079; Eukaryota.
DR InParanoid; Q580S0; -.
DR OMA; EMSVKEM; -.
DR Proteomes; UP000008524; Chromosome 3.
DR GO; GO:0036064; C:ciliary basal body; IDA:GeneDB.
DR GO; GO:0005929; C:cilium; IDA:GeneDB.
DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0030990; C:intraciliary transport particle; IDA:GeneDB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0005525; F:GTP binding; ISM:GeneDB.
DR GO; GO:0003924; F:GTPase activity; ISM:GeneDB.
DR GO; GO:0035720; P:intraciliary anterograde transport; IMP:UniProtKB.
DR GO; GO:0035721; P:intraciliary retrograde transport; IMP:UniProtKB.
DR GO; GO:0035735; P:intraciliary transport involved in cilium assembly; IMP:GeneDB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:1902017; P:regulation of cilium assembly; IMP:GeneDB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; ISM:GeneDB.
DR CDD; cd04101; RabL4; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR034112; RabL4_euk.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Flagellum; GTP-binding; Nucleotide-binding;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..183
FT /note="Intraflagellar transport protein 27 homolog"
FT /id="PRO_0000431686"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 63..67
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 120..123
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MUTAGEN 19
FT /note="T->N: GDP-locked, leading to impaired flagellum
FT assembly. Unable to enter the flagellum and to interact
FT with other IFT-B proteins."
FT MUTAGEN 67
FT /note="Q->L: GTP-locked."
SQ SEQUENCE 183 AA; 20639 MW; AF7127BC5D910634 CRC64;
MVNLRLQVAV VGAPTVGKTA FVQMLHSNGT TFPKNYLMTL GCDFIVKEVP VDDDNTVEMI
IFDVSGQREY EPMVSSYLQN TAVFIVMYDV SNKVTFEACA RWVNQVRTNS KESVGILIAN
KSDLSDKAEV TDRQGKDLAN ANKMKFYKIS TLRGVGITEP IDEIARHYVD AYQKRIEQLT
QMR
