IFT43_MOUSE
ID IFT43_MOUSE Reviewed; 206 AA.
AC Q9DA69; Q8CFI3; Q8K213;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Intraflagellar transport protein 43 homolog {ECO:0000305};
GN Name=Ift43 {ECO:0000312|MGI:MGI:1923661};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-206 (ISOFORMS 1 AND 3).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=25243405; DOI=10.1371/journal.pone.0108470;
RA Broekhuis J.R., Verhey K.J., Jansen G.;
RT "Regulation of cilium length and intraflagellar transport by the RCK-
RT kinases ICK and MOK in renal epithelial cells.";
RL PLoS ONE 9:E108470-E108470(2014).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=28973684; DOI=10.1093/hmg/ddx356;
RA Biswas P., Duncan J.L., Ali M., Matsui H., Naeem M.A., Raghavendra P.B.,
RA Frazer K.A., Arts H.H., Riazuddin S., Akram J., Hejtmancik J.F.,
RA Riazuddin S.A., Ayyagari R.;
RT "A mutation in IFT43 causes non-syndromic recessive retinal degeneration.";
RL Hum. Mol. Genet. 26:4741-4751(2017).
CC -!- FUNCTION: As a component of IFT complex A (IFT-A), a complex required
CC for retrograde ciliary transport and entry into cilia of G protein-
CC coupled receptors (GPCRs), it is involved in ciliogenesis. Involved in
CC retrograde ciliary transport along microtubules from the ciliary tip to
CC the base. {ECO:0000250|UniProtKB:Q96FT9}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) complex. IFT-A complex
CC is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which
CC is associated with TULP3 and a peripheral subcomplex composed of
CC IFT43:WDR35:TTC21B. Interacts directy with IFT122, WDR35 and TTC21B.
CC {ECO:0000250|UniProtKB:Q96FT9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q96FT9}. Cell projection, cilium
CC {ECO:0000269|PubMed:25243405}. Note=Associated with microtubules.
CC Localized at the distal tip of the cilium.
CC {ECO:0000250|UniProtKB:Q96FT9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9DA69-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9DA69-2; Sequence=VSP_021171;
CC Name=3;
CC IsoId=Q9DA69-3; Sequence=VSP_021170;
CC -!- TISSUE SPECIFICITY: Expressed in the retina, iris-ciliary body, lens
CC and cornea. Higher expression is observed in the retina, predominantly
CC in the photoreceptor outer segment. {ECO:0000269|PubMed:28973684}.
CC -!- SIMILARITY: Belongs to the IFT43 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH38915.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK006115; BAB24415.1; -; mRNA.
DR EMBL; BC034668; AAH34668.1; -; mRNA.
DR EMBL; BC038915; AAH38915.1; ALT_INIT; mRNA.
DR CCDS; CCDS26064.1; -. [Q9DA69-2]
DR CCDS; CCDS88375.1; -. [Q9DA69-1]
DR RefSeq; NP_001186772.1; NM_001199843.1. [Q9DA69-1]
DR RefSeq; NP_083877.1; NM_029601.3. [Q9DA69-2]
DR AlphaFoldDB; Q9DA69; -.
DR ComplexPortal; CPX-5027; IFT-A complex.
DR STRING; 10090.ENSMUSP00000061891; -.
DR iPTMnet; Q9DA69; -.
DR PhosphoSitePlus; Q9DA69; -.
DR MaxQB; Q9DA69; -.
DR PRIDE; Q9DA69; -.
DR ProteomicsDB; 269457; -. [Q9DA69-1]
DR ProteomicsDB; 269458; -. [Q9DA69-2]
DR ProteomicsDB; 269459; -. [Q9DA69-3]
DR Antibodypedia; 191; 87 antibodies from 20 providers.
DR DNASU; 76411; -.
DR Ensembl; ENSMUST00000054565; ENSMUSP00000061891; ENSMUSG00000007867. [Q9DA69-2]
DR Ensembl; ENSMUST00000222821; ENSMUSP00000152079; ENSMUSG00000007867. [Q9DA69-1]
DR GeneID; 76411; -.
DR KEGG; mmu:76411; -.
DR UCSC; uc007oho.2; mouse. [Q9DA69-2]
DR UCSC; uc007ohp.2; mouse. [Q9DA69-1]
DR CTD; 112752; -.
DR MGI; MGI:1923661; Ift43.
DR VEuPathDB; HostDB:ENSMUSG00000007867; -.
DR eggNOG; ENOG502RXJ2; Eukaryota.
DR GeneTree; ENSGT00390000012060; -.
DR HOGENOM; CLU_104337_0_0_1; -.
DR InParanoid; Q9DA69; -.
DR OMA; RKTGWGD; -.
DR OrthoDB; 1272229at2759; -.
DR PhylomeDB; Q9DA69; -.
DR TreeFam; TF323566; -.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 76411; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Ift43; mouse.
DR PRO; PR:Q9DA69; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q9DA69; protein.
DR Bgee; ENSMUSG00000007867; Expressed in interventricular septum and 229 other tissues.
DR ExpressionAtlas; Q9DA69; baseline and differential.
DR Genevisible; Q9DA69; MM.
DR GO; GO:0034451; C:centriolar satellite; ISO:MGI.
DR GO; GO:0005814; C:centriole; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0030991; C:intraciliary transport particle A; IDA:MGI.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0035721; P:intraciliary retrograde transport; ISS:UniProtKB.
DR InterPro; IPR029302; IFT43.
DR PANTHER; PTHR33724; PTHR33724; 1.
DR Pfam; PF15305; IFT43; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell projection;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..206
FT /note="Intraflagellar transport protein 43 homolog"
FT /id="PRO_0000254042"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q96FT9"
FT MOD_RES 77
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96FT9"
FT VAR_SEQ 1..18
FT /note="MDDLLDLGEERRRSSATS -> MLGAG (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_021170"
FT VAR_SEQ 71..82
FT /note="KLGKKVSEEMED -> N (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021171"
SQ SEQUENCE 206 AA; 23501 MW; 318C95F1D108B9FE CRC64;
MDDLLDLGEE RRRSSATSGA KMGRRAHQES TQAENYLNSK NSSLTQTGEA PPPKPPRRQG
GWADDSMKMA KLGKKVSEEM EDHRLRQQNL MGSDDDDIPV IPDLEDVQEE DFVLQVASPP
SIQVNRVMTY RDLDNDLMKY SAFQTLDGEI DLKLLTKVLA PEHEVREDDV GWDWDHLYTE
VSSELLTEWD LLRAEKEDPV GPSRYI
