IFT46_CAEEL
ID IFT46_CAEEL Reviewed; 471 AA.
AC Q0G838; G5EDH6; G5EF67; Q1KYM3; Q1KYP5; Q20474;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Intraflagellar transport protein 46 homolog {ECO:0000250|UniProtKB:Q9NQC8};
DE AltName: Full=Abnormal dye filling protein 6 {ECO:0000312|WormBase:F46F6.4a};
GN Name=dyf-6 {ECO:0000312|WormBase:F46F6.4a};
GN ORFNames=F46F6.4 {ECO:0000312|WormBase:F46F6.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:ABC88649.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16648645; DOI=10.1534/genetics.106.056721;
RA Bell L.R., Stone S., Yochem J., Shaw J.E., Herman R.K.;
RT "The molecular identities of the Caenorhabditis elegans intraflagellar
RT transport genes dyf-6, daf-10 and osm-1.";
RL Genetics 173:1275-1286(2006).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=7705621; DOI=10.1093/genetics/139.1.171;
RA Starich T.A., Herman R.K., Kari C.K., Yeh W.H., Schackwitz W.S.,
RA Schuyler M.W., Collet J., Thomas J.H., Riddle D.L.;
RT "Mutations affecting the chemosensory neurons of Caenorhabditis elegans.";
RL Genetics 139:171-188(1995).
RN [4]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX B, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B
CC required for transport of proteins in the motile cilium
CC (PubMed:28479320). May be required for ciliary entrance and transport
CC of specific ciliary cargo proteins such as che-3 which are related to
CC motility (PubMed:28479320). Required for normal morphology and function
CC of ciliated amphid sensory neurons (PubMed:16648645, PubMed:7705621).
CC {ECO:0000269|PubMed:16648645, ECO:0000269|PubMed:28479320,
CC ECO:0000269|PubMed:7705621}.
CC -!- SUBUNIT: Component of the IFT complex B composed of at least che-2,
CC che-13, dyf-1, dyf-3, dyf-6, dyf-11, dyf-13, ift-20, ift-74, ift-81,
CC ifta-2, osm-1, osm-5 and osm-6. {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:16648645}. Cytoplasm, cytoskeleton, cilium basal
CC body {ECO:0000269|PubMed:16648645}. Cell projection, dendrite
CC {ECO:0000269|PubMed:16648645}. Perikaryon
CC {ECO:0000269|PubMed:16648645}. Note=Highly expressed in the transition
CC zones between the cilium basal body and the dendrites.
CC {ECO:0000269|PubMed:16648645}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a {ECO:0000312|WormBase:F46F6.4a};
CC IsoId=Q0G838-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F46F6.4b}; Synonyms=c
CC {ECO:0000312|WormBase:F46F6.4c};
CC IsoId=Q0G838-2; Sequence=VSP_058390;
CC Name=c {ECO:0000312|WormBase:F46F6.4c};
CC IsoId=Q0G838-3; Sequence=VSP_058391, VSP_058392;
CC Name=d {ECO:0000312|WormBase:F46F6.4d};
CC IsoId=Q0G838-4; Sequence=VSP_058390, VSP_058393;
CC -!- TISSUE SPECIFICITY: Expressed in the hypodermis and sensory neurons
CC including inner labial, PDE, amphid and phasmid neurons.
CC {ECO:0000269|PubMed:16648645}.
CC -!- DEVELOPMENTAL STAGE: Expressed from hatching to adulthood. Expressed in
CC dauer larvae. {ECO:0000269|PubMed:16648645}.
CC -!- DISRUPTION PHENOTYPE: Shorter and compacted dendritic endings of the
CC amphid and phasmid neurons with an abnormal distribution of the
CC intraflagellar transport protein osm-6 along these ciliated endings
CC (PubMed:16648645). Defective chemotaxis with 78% of mutants exhibiting
CC reduced tracking to the chemoattractant ammonium chloride
CC (PubMed:7705621). {ECO:0000269|PubMed:16648645,
CC ECO:0000269|PubMed:7705621}.
CC -!- SIMILARITY: Belongs to the IFT46 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABC88649.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABD14369.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ360812; ABC88649.1; ALT_INIT; mRNA.
DR EMBL; DQ360813; ABC88650.1; -; mRNA.
DR EMBL; DQ365983; ABD14368.1; -; mRNA.
DR EMBL; DQ365984; ABD14369.1; ALT_INIT; mRNA.
DR EMBL; BX284606; CAA90337.1; -; Genomic_DNA.
DR EMBL; BX284606; CAL36517.1; -; Genomic_DNA.
DR EMBL; BX284606; CAL36518.1; -; Genomic_DNA.
DR EMBL; BX284606; CAL36519.1; -; Genomic_DNA.
DR PIR; T22317; T22317.
DR RefSeq; NP_001076767.1; NM_001083298.1. [Q0G838-1]
DR RefSeq; NP_001076768.1; NM_001083299.2. [Q0G838-3]
DR RefSeq; NP_001076769.1; NM_001083300.2.
DR RefSeq; NP_001076770.1; NM_001083301.2. [Q0G838-4]
DR AlphaFoldDB; Q0G838; -.
DR SMR; Q0G838; -.
DR ComplexPortal; CPX-1290; Intraflagellar transport complex B.
DR STRING; 6239.F46F6.4a; -.
DR EPD; Q0G838; -.
DR PaxDb; Q0G838; -.
DR EnsemblMetazoa; F46F6.4a.1; F46F6.4a.1; WBGene00001122. [Q0G838-1]
DR EnsemblMetazoa; F46F6.4b.1; F46F6.4b.1; WBGene00001122. [Q0G838-3]
DR EnsemblMetazoa; F46F6.4c.1; F46F6.4c.1; WBGene00001122. [Q0G838-2]
DR EnsemblMetazoa; F46F6.4c.2; F46F6.4c.2; WBGene00001122. [Q0G838-2]
DR EnsemblMetazoa; F46F6.4d.1; F46F6.4d.1; WBGene00001122. [Q0G838-4]
DR GeneID; 3565899; -.
DR KEGG; cel:CELE_F46F6.4; -.
DR UCSC; F46F6.4d; c. elegans.
DR CTD; 3565899; -.
DR WormBase; F46F6.4a; CE40388; WBGene00001122; dyf-6. [Q0G838-1]
DR WormBase; F46F6.4b; CE02236; WBGene00001122; dyf-6. [Q0G838-3]
DR WormBase; F46F6.4c; CE40389; WBGene00001122; dyf-6. [Q0G838-2]
DR WormBase; F46F6.4d; CE40390; WBGene00001122; dyf-6. [Q0G838-4]
DR eggNOG; ENOG502QPNA; Eukaryota.
DR GeneTree; ENSGT00390000005544; -.
DR InParanoid; Q0G838; -.
DR OMA; TQIDEPP; -.
DR OrthoDB; 1245404at2759; -.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:Q0G838; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001122; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005929; C:cilium; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IDA:WormBase.
DR GO; GO:0030992; C:intraciliary transport particle B; IBA:GO_Central.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IDA:WormBase.
DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR GO; GO:0042073; P:intraciliary transport; IDA:WormBase.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:WormBase.
DR InterPro; IPR022088; Intraflagellar_transp_cmplxB.
DR PANTHER; PTHR13376; PTHR13376; 1.
DR Pfam; PF12317; IFT46_B_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Reference proteome; Transport.
FT CHAIN 1..471
FT /note="Intraflagellar transport protein 46 homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436569"
FT REGION 1..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 226..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 232..246
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..151
FT /note="Missing (in isoform b and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_058390"
FT VAR_SEQ 147..158
FT /note="ALENPMAANGEF -> VTLFIEAFINQK (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_058391"
FT VAR_SEQ 159..471
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_058392"
FT VAR_SEQ 202..223
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_058393"
FT CONFLICT 1
FT /note="M -> MLTM (in Ref. 1; ABC88649)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 471 AA; 53420 MW; DF615C1CAC35A9AA CRC64;
MSSETTAPMI INEKSSLPVK SEPGQLGNPI SDESSEDEQS QIRALEEDQG IYYNEEVVAS
PKNESDDDIP LRRIQLSHQT STQQHPEDSE PQEVIDVNDI ELPAGEAQPN MERRRSVRFS
GRHDEEEDGR NKHFRTPSPE SLRYIQALEN PMAANGEFEE EFNDLADPQV QPPPMGSPPA
YTSADEGPKT PPPRASAGSN MRQESMNELI MRKISDPLQN LIRRASRLED DSSNDDDDDD
DDEDDDYTED EIAILTYIDA YKTQEVELRP QLRPFTIEYI PAMGDVDLFI KVPRPDEIDD
NVGLTQIDEP PSNQSDATIV DMQIRNATKD AAILDDDVPV KLLERADENP DEIKKWISDI
KEFHKSKPAQ TVHYRTQLPD VETLMQEWPQ KLEEVLKTTK IPSAELDVSL EKYVEICLNI
VDIPVGKSRI EALHLMFSLL NEFNNSQHFR NLAQNNNLGG ETGETMDRLE L
