IFT46_DANRE
ID IFT46_DANRE Reviewed; 384 AA.
AC A7MBP4; Q0P418;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Intraflagellar transport protein 46 homolog {ECO:0000250|UniProtKB:Q9DB07, ECO:0000312|ZFIN:ZDB-GENE-080102-3};
GN Name=ift46 {ECO:0000312|EMBL:AAI51862.1,
GN ECO:0000312|ZFIN:ZDB-GENE-080102-3};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAI51862.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-384.
RC TISSUE=Olfactory epithelium {ECO:0000312|EMBL:AAI22333.1}, and
RC Testis {ECO:0000312|EMBL:AAI51862.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17720815; DOI=10.1074/jbc.m705730200;
RA Gouttenoire J., Valcourt U., Bougault C., Aubert-Foucher E., Arnaud E.,
RA Giraud L., Mallein-Gerin F.;
RT "Knockdown of the intraflagellar transport protein IFT46 stimulates
RT selective gene expression in mouse chondrocytes and affects early
RT development in zebrafish.";
RL J. Biol. Chem. 282:30960-30973(2007).
CC -!- FUNCTION: Forms part of a complex involved in intraflagellar transport
CC (IFT), the bi-directional movement of particles required for the
CC assembly, maintenance and functioning of primary cilia. Plays a role in
CC early embryonic development. {ECO:0000250|UniProtKB:Q9DB07,
CC ECO:0000269|PubMed:17720815}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000250|UniProtKB:Q9DB07}. Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q9DB07}.
CC -!- DEVELOPMENTAL STAGE: Expressed ubiquitously during early embryonic
CC development. Expression is detected at 3 hours post fertilization (hpf)
CC and reaches a peak at 20 hpf. {ECO:0000269|PubMed:17720815}.
CC -!- DISRUPTION PHENOTYPE: Individuals display a number of developmental
CC defects, including dorsalization and formation of ectopic tail.
CC {ECO:0000269|PubMed:17720815}.
CC -!- SIMILARITY: Belongs to the IFT46 family. {ECO:0000255}.
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DR EMBL; CU570991; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC122332; AAI22333.1; -; mRNA.
DR EMBL; BC151861; AAI51862.1; -; mRNA.
DR AlphaFoldDB; A7MBP4; -.
DR SMR; A7MBP4; -.
DR STRING; 7955.ENSDARP00000083038; -.
DR PaxDb; A7MBP4; -.
DR PeptideAtlas; A7MBP4; -.
DR ZFIN; ZDB-GENE-080102-3; ift46.
DR eggNOG; ENOG502QPNA; Eukaryota.
DR InParanoid; A7MBP4; -.
DR PhylomeDB; A7MBP4; -.
DR Reactome; R-DRE-5620924; Intraflagellar transport.
DR PRO; PR:A7MBP4; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0036064; C:ciliary basal body; IDA:ZFIN.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR GO; GO:0048264; P:determination of ventral identity; IMP:ZFIN.
DR GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR GO; GO:0048793; P:pronephros development; IMP:ZFIN.
DR GO; GO:0060041; P:retina development in camera-type eye; IMP:ZFIN.
DR InterPro; IPR022088; Intraflagellar_transp_cmplxB.
DR PANTHER; PTHR13376; PTHR13376; 1.
DR Pfam; PF12317; IFT46_B_C; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..384
FT /note="Intraflagellar transport protein 46 homolog"
FT /id="PRO_0000399499"
FT REGION 52..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..109
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..141
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 153
FT /note="H -> R (in Ref. 2; AAI51862)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="F -> S (in Ref. 2; AAI51862)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="K -> R (in Ref. 2; AAI51862)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 43342 MW; 2CF853240CD34B56 CRC64;
MASVAYICSL PVNTSRDQVS LATVHRLVFM ERSERQKIQL TTNQPYDESF EVNAEEAASV
HTPSPRQIVS RRTGRQKQST MSGYAGEKLE EDTKRKKEPP PGPRGANKND EEEDEDDDDD
DDDDDSDDTE SDEEEEEPGS APEGAYDPAD YEHLPVTGEI KELFQYITRY TPQTIELDHK
LKPFIPDFIP AVGDIDAFLK VPRPDGKPDN LGLLVLDEPC TKQSDPTVLS LWLSENSKQH
NVTEVKVKSI ENPEKNPKAI DNWIESISEL HRSKPPATVH YTRPMPDIDS LMQEWPSEFE
ELLGKVNLPT ADIDCDLAEY VDMICGILDI PVYKNRIHSL HVLFTLYSEF KNSQHFKSAT
DGQKSDTPPA SRSATAEIER LTLD
