IFT56_DANRE
ID IFT56_DANRE Reviewed; 557 AA.
AC Q5PR66; F1QZ19;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-JAN-2015, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Intraflagellar transport protein 56 {ECO:0000303|PubMed:24596149};
DE AltName: Full=Tetratricopeptide repeat protein 26;
DE Short=TPR repeat protein 26;
GN Name=ttc26; Synonyms=ift56 {ECO:0000303|PubMed:24596149};
GN ORFNames=zgc:103412;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22718903; DOI=10.1091/mbc.e12-01-0019;
RA Zhang Q., Liu Q., Austin C., Drummond I., Pierce E.A.;
RT "Knockdown of ttc26 disrupts ciliogenesis of the photoreceptor cells and
RT the pronephros in zebrafish.";
RL Mol. Biol. Cell 23:3069-3078(2012).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=24596149; DOI=10.7554/elife.01566;
RA Ishikawa H., Ide T., Yagi T., Jiang X., Hirono M., Sasaki H.,
RA Yanagisawa H., Wemmer K.A., Stainier D.Y., Qin H., Kamiya R.,
RA Marshall W.F.;
RT "TTC26/DYF13 is an intraflagellar transport protein required for transport
RT of motility-related proteins into flagella.";
RL Elife 3:E01566-E01566(2014).
RN [5]
RP ERRATUM OF PUBMED:24596149.
RX PubMed=24692455; DOI=10.7554/elife.02897;
RA Ishikawa H., Ide T., Yagi T., Jiang X., Hirono M., Sasaki H.,
RA Yanagisawa H., Wemmer K.A., Stainier D.Y., Qin H., Kamiya R.,
RA Marshall W.F.;
RL Elife 3:E02897-E02897(2014).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B
CC required for transport of proteins in the motile cilium. Required for
CC transport of specific ciliary cargo proteins related to motility, while
CC it is neither required for IFT complex B assembly or motion nor for
CC cilium assembly. Plays a key role in maintaining the integrity of the
CC IFT complex B and the proper ciliary localization of the IFT complex B
CC components. Essential for maintaining proper microtubule organization
CC within the ciliary axoneme (By similarity).
CC {ECO:0000250|UniProtKB:Q8BS45, ECO:0000269|PubMed:22718903,
CC ECO:0000305|PubMed:24596149}.
CC -!- SUBUNIT: Component of the IFT complex B.
CC {ECO:0000250|UniProtKB:Q8BS45}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000250|UniProtKB:Q8BS45}.
CC -!- DISRUPTION PHENOTYPE: Defective cilia (PubMed:22718903,
CC PubMed:24596149). Morpholino knockdown of the protein results in cilia
CC defects in the pronephric kidney at 27 hpf and distension/dilation of
CC pronephros at 5 dpf. In the eyes, the outer segments of photoreceptor
CC cells appear shortened or absent, whereas cellular lamination seems
CC normal in retinas at 5 dpf (PubMed:22718903). Left-right asymmetry
CC defects with abnormal heart looping, hydrocephalus, pronephric cysts,
CC abnormal ear otolith formation and curly body axis (PubMed:24596149).
CC {ECO:0000269|PubMed:22718903, ECO:0000269|PubMed:24596149}.
CC -!- SIMILARITY: Belongs to the IFT56 family. {ECO:0000305}.
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DR EMBL; AL954384; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC086810; AAH86810.1; -; mRNA.
DR RefSeq; NP_001008617.1; NM_001008617.1.
DR AlphaFoldDB; Q5PR66; -.
DR SMR; Q5PR66; -.
DR STRING; 7955.ENSDARP00000032176; -.
DR PaxDb; Q5PR66; -.
DR Ensembl; ENSDART00000032494; ENSDARP00000032176; ENSDARG00000012039.
DR GeneID; 494074; -.
DR KEGG; dre:494074; -.
DR CTD; 79989; -.
DR ZFIN; ZDB-GENE-041212-41; ttc26.
DR eggNOG; KOG3785; Eukaryota.
DR GeneTree; ENSGT00390000000159; -.
DR HOGENOM; CLU_036306_2_0_1; -.
DR InParanoid; Q5PR66; -.
DR OMA; FIIRRDY; -.
DR OrthoDB; 383516at2759; -.
DR PhylomeDB; Q5PR66; -.
DR TreeFam; TF105816; -.
DR Reactome; R-DRE-5620924; Intraflagellar transport.
DR PRO; PR:Q5PR66; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000012039; Expressed in testis and 35 other tissues.
DR GO; GO:0036064; C:ciliary basal body; IBA:GO_Central.
DR GO; GO:0097546; C:ciliary base; IBA:GO_Central.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:0120170; F:intraciliary transport particle B binding; IBA:GO_Central.
DR GO; GO:0035082; P:axoneme assembly; ISS:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IDA:MGI.
DR GO; GO:0035720; P:intraciliary anterograde transport; IBA:GO_Central.
DR GO; GO:0042073; P:intraciliary transport; IMP:UniProtKB.
DR GO; GO:0035735; P:intraciliary transport involved in cilium assembly; IBA:GO_Central.
DR GO; GO:0046530; P:photoreceptor cell differentiation; IDA:MGI.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IDA:MGI.
DR GO; GO:0035845; P:photoreceptor cell outer segment organization; IMP:ZFIN.
DR GO; GO:0039023; P:pronephric duct morphogenesis; IMP:ZFIN.
DR GO; GO:0061512; P:protein localization to cilium; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007224; P:smoothened signaling pathway; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR030511; TTC26.
DR PANTHER; PTHR14781; PTHR14781; 1.
DR SMART; SM00028; TPR; 4.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Protein transport; Reference proteome; Repeat;
KW TPR repeat; Transport.
FT CHAIN 1..557
FT /note="Intraflagellar transport protein 56"
FT /id="PRO_0000289086"
FT REPEAT 60..93
FT /note="TPR 1"
FT REPEAT 95..128
FT /note="TPR 2"
FT REPEAT 154..187
FT /note="TPR 3"
FT REPEAT 471..504
FT /note="TPR 4"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 274
FT /note="E -> G (in Ref. 2; AAH86810)"
SQ SEQUENCE 557 AA; 64186 MW; DCED6622EFF8BFBE CRC64;
MLLSRMKPAV GGEASTSSNE KKRKNKSKKI PRLEDYLNQR DYLGALTLLE FQRNSGVSVE
HADLWTGFCA FHVGDHKRAM EEYKALTLRP DCPVDVWVYL GCALFFLGLY KEAEEAALKG
SKTQLQNRLL FHLAHKFNDE KKLMGFHQNL EDVTEDQLSL ASIHYMRSHY QEAIDIYKRI
LLQNREFLAL NVYVALCYYK LDYYDVSQEV LAVYLQSIPD STIALNLKAC NHFRLYNGKA
AETELKNLID ISSSSFQFAK ELIQHNLVVF RGGEGALQVL PPLIDVISEA RLNLVIYYLR
QDDIQEAYKL IKDLEPTTPQ EYILKGVVNA ALGQEIGSRD HLKIAQQFFQ LVGGSASECD
TIPGRQCMAS CFFLLKQFED VLIYLNSVKS YFYNDDTFSF NYAQAKAALG NYREAEELFL
LIQNEKIKSD YVFQSWLARC YIMNQKPRQA WELYLRMETS SDPFSLLQLI ANDCYKMGQF
YYAAKAFDAL ERLDPNPEYW EGKRGACVGI FQLILAGRES REILKEVLPM LRSTGNPQVE
YIIRIMKKWA KDNRVAL
