IFT74_MOUSE
ID IFT74_MOUSE Reviewed; 600 AA.
AC Q8BKE9; B1AWG2; Q80ZI3; Q9CSY1; Q9CUS0; Q9D9T5;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Intraflagellar transport protein 74 homolog;
DE AltName: Full=Capillary morphogenesis gene 1 protein;
DE Short=CMG-1;
DE AltName: Full=Coiled-coil domain-containing protein 2;
GN Name=Ift74; Synonyms=Ccdc2, Cmg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Eye, Head, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT88, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16775004; DOI=10.1091/mbc.e06-02-0133;
RA Follit J.A., Tuft R.A., Fogarty K.E., Pazour G.J.;
RT "The intraflagellar transport protein IFT20 is associated with the Golgi
RT complex and is required for cilia assembly.";
RL Mol. Biol. Cell 17:3781-3792(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-73, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E.,
RA Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y.,
RA Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP IDENTIFICATION IN THE IFT COMPLEX B, AND INTERACTION WITH IFT57 AND TTC30B.
RX PubMed=23810713; DOI=10.1016/j.yexcr.2013.06.010;
RA Howard P.W., Jue S.F., Maurer R.A.;
RT "Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport
RT complex B proteins and KIF17.";
RL Exp. Cell Res. 319:2275-2281(2013).
RN [7]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-51, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=31004481; DOI=10.1093/biolre/ioz071;
RA Shi L., Zhou T., Huang Q., Zhang S., Li W., Zhang L., Hess R.A.,
RA Pazour G.J., Zhang Z.;
RT "Intraflagellar transport protein 74 is essential for spermatogenesis and
RT male fertility in mice.";
RL Biol. Reprod. 101:188-199(2019).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B:
CC together with IFT81, forms a tubulin-binding module that specifically
CC mediates transport of tubulin within the cilium. Binds beta-tubulin via
CC its basic region. Required for ciliogenesis (By similarity). Essential
CC for flagellogenesis during spermatogenesis (PubMed:31004481).
CC {ECO:0000250|UniProtKB:Q96LB3, ECO:0000269|PubMed:31004481}.
CC -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74,
CC IFT80, IFT81, and IFT88 (PubMed:16775004, PubMed:23810713). Interacts
CC with IFT81; the interaction is direct: within the IFT complex B, IFT74
CC and IFT81 mediate the transport of tubulin within the cilium. Interacts
CC (via basic region) with beta-tubulin (via acidic region); interaction
CC is direct. Interacts with ARL13B and IFT88. Interacts (via the
CC IFT74/IFT81 heterodimer) with RABL2B (By similarity). Interacts with
CC IFT57 and TTC30B (PubMed:23810713). {ECO:0000250|UniProtKB:Q96LB3,
CC ECO:0000269|PubMed:16775004, ECO:0000269|PubMed:23810713}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:16775004}. Cytoplasmic vesicle
CC {ECO:0000250|UniProtKB:Q96LB3}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:31004481}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000269|PubMed:31004481}. Note=In male germ cells,
CC strongly expressed in the vesicles of spermatocytes and round
CC spermatids and also in the acrosome and centrosome regions of
CC elongating spermatids and in developing sperm tails.
CC {ECO:0000269|PubMed:31004481}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in testis, but also
CC detected in other organs containing cilia-bearing cells, including
CC lung, brain and kidney (at protein level).
CC {ECO:0000269|PubMed:31004481}.
CC -!- DEVELOPMENTAL STAGE: In testis, expression starts at day 12 and
CC strongly increases on day 20 and thereafter, temporally correlating
CC with the first wave of spermatogenesis. {ECO:0000269|PubMed:31004481}.
CC -!- SIMILARITY: Belongs to the IFT74 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24617.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK006492; BAB24617.1; ALT_INIT; mRNA.
DR EMBL; AK011690; BAB27780.1; -; mRNA.
DR EMBL; AK014770; BAB29542.3; -; mRNA.
DR EMBL; AK053369; BAC35365.1; -; mRNA.
DR EMBL; AL732597; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL772277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51232.1; -.
DR RefSeq; NP_001277497.1; NM_001290568.1.
DR RefSeq; NP_080595.2; NM_026319.3.
DR RefSeq; XP_006503387.1; XM_006503324.3.
DR RefSeq; XP_006503389.1; XM_006503326.2.
DR RefSeq; XP_011238899.1; XM_011240597.2.
DR AlphaFoldDB; Q8BKE9; -.
DR SMR; Q8BKE9; -.
DR BioGRID; 212373; 2.
DR ComplexPortal; CPX-5028; IFT-B complex.
DR STRING; 10090.ENSMUSP00000030311; -.
DR iPTMnet; Q8BKE9; -.
DR PhosphoSitePlus; Q8BKE9; -.
DR MaxQB; Q8BKE9; -.
DR PaxDb; Q8BKE9; -.
DR PRIDE; Q8BKE9; -.
DR ProteomicsDB; 267286; -.
DR Antibodypedia; 5487; 199 antibodies from 32 providers.
DR DNASU; 67694; -.
DR Ensembl; ENSMUST00000030311; ENSMUSP00000030311; ENSMUSG00000028576.
DR GeneID; 67694; -.
DR KEGG; mmu:67694; -.
DR UCSC; uc008tsg.2; mouse.
DR CTD; 80173; -.
DR MGI; MGI:1914944; Ift74.
DR VEuPathDB; HostDB:ENSMUSG00000028576; -.
DR eggNOG; ENOG502QS4E; Eukaryota.
DR GeneTree; ENSGT00390000007109; -.
DR HOGENOM; CLU_027673_2_0_1; -.
DR InParanoid; Q8BKE9; -.
DR OMA; MQDDLSF; -.
DR OrthoDB; 622935at2759; -.
DR PhylomeDB; Q8BKE9; -.
DR TreeFam; TF318352; -.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 67694; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Ift74; mouse.
DR PRO; PR:Q8BKE9; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BKE9; protein.
DR Bgee; ENSMUSG00000028576; Expressed in seminiferous tubule of testis and 250 other tissues.
DR ExpressionAtlas; Q8BKE9; baseline and differential.
DR Genevisible; Q8BKE9; MM.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0030992; C:intraciliary transport particle B; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0008544; P:epidermis development; IMP:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0035720; P:intraciliary anterograde transport; IC:ComplexPortal.
DR GO; GO:0042073; P:intraciliary transport; IC:MGI.
DR GO; GO:0035735; P:intraciliary transport involved in cilium assembly; ISS:UniProtKB.
DR GO; GO:0003334; P:keratinocyte development; IMP:MGI.
DR GO; GO:0043616; P:keratinocyte proliferation; IMP:MGI.
DR GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IMP:MGI.
DR GO; GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:MGI.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR GO; GO:0007219; P:Notch signaling pathway; IMP:MGI.
DR GO; GO:0033630; P:positive regulation of cell adhesion mediated by integrin; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR InterPro; IPR029602; IFT74.
DR PANTHER; PTHR31432; PTHR31432; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW Cytoplasmic vesicle; Developmental protein; Flagellum; Methylation;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..600
FT /note="Intraflagellar transport protein 74 homolog"
FT /id="PRO_0000084170"
FT REGION 1..90
FT /note="Basic region"
FT /evidence="ECO:0000250"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 97..390
FT /evidence="ECO:0000255"
FT MOD_RES 51
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 73
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17525332"
SQ SEQUENCE 600 AA; 69301 MW; 671606E5CA9096D5 CRC64;
MASNHKSSAP RPISRGGIGL TGRPPSGIRP PSGNVRVATA MPPTTARPGS RGGPLGTGGV
LSSQIKVADR PVTQQGLSGM KTGMKGPQRQ ILDKSYYLGL LRSKISELTT EINKLQKEIE
MYNQENSVYL SYEKRAETLA VEIKDFQGQL ADYNMLVDKL NTNTEMEEVM SDYNMLKAQN
DRETQSMDVI FTERQAKEKQ IRSVEEEVEQ EKQAADGIIK NMSPEKQVKY IEMKTTNEKL
LQELDTLQQQ LDSLNMKKES LETEIAHSQV KQEAVLLYEK LYELESHRDQ MIAEDKSMGS
PMEERERLLK QVKEDNQEIA SMERQLTDIK EKINQFSEEI RQLDMDLEEH QGEMNQKYKE
LKKREENMDA FIETFEETKN QELERKAQIE ASIITLLEHC SRNINRMKQI SSITNQELKM
MQDDLSFKST EMQKSQTTAR NLTSDSQRLQ LDLQKMELLE SKMTEEQQSL KNKIKQMTAD
LETYSDLAAL KSSAEEKKKK LHQERTVLST HRNAFKKIME KLTSDYDTLK RQLQDNETHA
QLTNLERKWQ HLEQNNFVMK EFIATKSQES DYQPVIKNVM KQIAEYNKTI MDALHNASRS
