IFT80_MOUSE
ID IFT80_MOUSE Reviewed; 777 AA.
AC Q8K057; Q6ZPT0; Q8C9F1; Q91YV4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Intraflagellar transport protein 80 homolog;
DE AltName: Full=WD repeat-containing protein 56;
GN Name=Ift80; Synonyms=Kiaa1374, Wdr56;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-751 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=17468754; DOI=10.1038/ng2038;
RA Beales P.L., Bland E., Tobin J.L., Bacchelli C., Tuysuz B., Hill J.,
RA Rix S., Pearson C.G., Kai M., Hartley J., Johnson C., Irving M.,
RA Elcioglu N., Winey M., Tada M., Scambler P.J.;
RT "IFT80, which encodes a conserved intraflagellar transport protein, is
RT mutated in Jeune asphyxiating thoracic dystrophy.";
RL Nat. Genet. 39:727-729(2007).
RN [5]
RP IDENTIFICATION IN THE IFT COMPLEX B, INTERACTION WITH IFT88, AND
RP SUBCELLULAR LOCATION.
RX PubMed=19253336; DOI=10.1002/cm.20346;
RA Follit J.A., Xu F., Keady B.T., Pazour G.J.;
RT "Characterization of mouse IFT complex B.";
RL Cell Motil. Cytoskeleton 66:457-468(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP IDENTIFICATION IN THE IFT COMPLEX B, AND INTERACTION WITH IFT57 AND TTC30B.
RX PubMed=23810713; DOI=10.1016/j.yexcr.2013.06.010;
RA Howard P.W., Jue S.F., Maurer R.A.;
RT "Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport
RT complex B proteins and KIF17.";
RL Exp. Cell Res. 319:2275-2281(2013).
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B,
CC which is essential for the development and maintenance of motile and
CC sensory cilia. {ECO:0000250}.
CC -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74,
CC IFT80, IFT81, and IFT88 (PubMed:19253336, PubMed:23810713). Interacts
CC with IFT88 (PubMed:19253336). Interacts with IFT57 and TTC30B
CC (PubMed:23810713). {ECO:0000269|PubMed:19253336,
CC ECO:0000269|PubMed:23810713}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, cilium basal
CC body. Cytoplasm, cytoskeleton, cilium axoneme. Note=Basal body and
CC ciliary axoneme in the chondrocytic ATDC-5 cell line.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K057-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K057-2; Sequence=VSP_027991, VSP_027992;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98149.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK129339; BAC98149.1; ALT_INIT; mRNA.
DR EMBL; BC013814; AAH13814.1; -; mRNA.
DR EMBL; BC034101; AAH34101.1; -; mRNA.
DR EMBL; AK042231; BAC31200.1; -; mRNA.
DR CCDS; CCDS38451.1; -. [Q8K057-1]
DR RefSeq; NP_080917.1; NM_026641.2. [Q8K057-1]
DR RefSeq; XP_006502029.1; XM_006501966.3. [Q8K057-1]
DR AlphaFoldDB; Q8K057; -.
DR SMR; Q8K057; -.
DR BioGRID; 212765; 5.
DR ComplexPortal; CPX-5028; IFT-B complex.
DR STRING; 10090.ENSMUSP00000133263; -.
DR iPTMnet; Q8K057; -.
DR PhosphoSitePlus; Q8K057; -.
DR EPD; Q8K057; -.
DR MaxQB; Q8K057; -.
DR PaxDb; Q8K057; -.
DR PRIDE; Q8K057; -.
DR ProteomicsDB; 267214; -. [Q8K057-1]
DR ProteomicsDB; 267215; -. [Q8K057-2]
DR Antibodypedia; 50241; 78 antibodies from 17 providers.
DR DNASU; 68259; -.
DR Ensembl; ENSMUST00000029347; ENSMUSP00000029347; ENSMUSG00000027778. [Q8K057-1]
DR Ensembl; ENSMUST00000107812; ENSMUSP00000103442; ENSMUSG00000027778. [Q8K057-1]
DR Ensembl; ENSMUST00000169064; ENSMUSP00000133263; ENSMUSG00000027778. [Q8K057-1]
DR GeneID; 68259; -.
DR KEGG; mmu:68259; -.
DR UCSC; uc033hub.1; mouse. [Q8K057-1]
DR CTD; 57560; -.
DR MGI; MGI:1915509; Ift80.
DR VEuPathDB; HostDB:ENSMUSG00000027778; -.
DR eggNOG; KOG1524; Eukaryota.
DR GeneTree; ENSGT00440000033499; -.
DR HOGENOM; CLU_024638_1_0_1; -.
DR InParanoid; Q8K057; -.
DR OMA; WDAQGAN; -.
DR OrthoDB; 203079at2759; -.
DR PhylomeDB; Q8K057; -.
DR TreeFam; TF106117; -.
DR Reactome; R-MMU-5620924; Intraflagellar transport.
DR BioGRID-ORCS; 68259; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Ift80; mouse.
DR PRO; PR:Q8K057; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8K057; protein.
DR Bgee; ENSMUSG00000027778; Expressed in dorsal pancreas and 244 other tissues.
DR ExpressionAtlas; Q8K057; baseline and differential.
DR Genevisible; Q8K057; MM.
DR GO; GO:0097731; C:9+0 non-motile cilium; IDA:MGI.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0036064; C:ciliary basal body; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030992; C:intraciliary transport particle B; IDA:UniProtKB.
DR GO; GO:0061975; P:articular cartilage development; IMP:MGI.
DR GO; GO:0035630; P:bone mineralization involved in bone maturation; IMP:MGI.
DR GO; GO:0060349; P:bone morphogenesis; IMP:MGI.
DR GO; GO:0002062; P:chondrocyte differentiation; IMP:MGI.
DR GO; GO:0060271; P:cilium assembly; IMP:MGI.
DR GO; GO:0001958; P:endochondral ossification; IMP:MGI.
DR GO; GO:0003418; P:growth plate cartilage chondrocyte differentiation; IMP:MGI.
DR GO; GO:0003417; P:growth plate cartilage development; IMP:MGI.
DR GO; GO:0035720; P:intraciliary anterograde transport; IC:ComplexPortal.
DR GO; GO:0042073; P:intraciliary transport; IC:MGI.
DR GO; GO:0043616; P:keratinocyte proliferation; IMP:MGI.
DR GO; GO:0060173; P:limb development; IMP:MGI.
DR GO; GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:MGI.
DR GO; GO:2000051; P:negative regulation of non-canonical Wnt signaling pathway; IGI:MGI.
DR GO; GO:0035567; P:non-canonical Wnt signaling pathway; IGI:MGI.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR GO; GO:0001649; P:osteoblast differentiation; IMP:MGI.
DR GO; GO:0033687; P:osteoblast proliferation; IMP:MGI.
DR GO; GO:0097500; P:receptor localization to non-motile cilium; IMP:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0007224; P:smoothened signaling pathway; IMP:MGI.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..777
FT /note="Intraflagellar transport protein 80 homolog"
FT /id="PRO_0000051043"
FT REPEAT 12..50
FT /note="WD 1"
FT REPEAT 104..143
FT /note="WD 2"
FT REPEAT 145..185
FT /note="WD 3"
FT REPEAT 186..225
FT /note="WD 4"
FT REPEAT 227..265
FT /note="WD 5"
FT REPEAT 267..306
FT /note="WD 6"
FT REPEAT 504..542
FT /note="WD 7"
FT VAR_SEQ 439
FT /note="I -> K (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_027991"
FT VAR_SEQ 440
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_027992"
SQ SEQUENCE 777 AA; 87811 MW; 2B01621A12E660C4 CRC64;
MRLKISLSKE PKHQELVSCV GWTTAEELYS CSDDHQIVKW NLLTSETSLI VKLPDDIYPI
DLHWFPKSLG IKKQTQAESF VLTSSDGKFH LISKLGRVEK SVEAHCGAVL AGRWNYEGTA
LVTVGEDGQV KIWSKTGMLR STLAQQGTPV YSVAWGPDSE KVLYTAGKQL IIKPLQPNAK
VLQWKAHDGI ILKVDWNSVN DLILSAGEDC KYKVWDSYGR VLYGSQPHEH PITSVAWAPD
GELFAVGSFH TLRLCDKTGW SYALEKPNTG SIFNIAWSID GTQIAGACGN GHVVFAHVVE
QRWEWKNFQV TLTKRRTMQV RNVLNDAVDL LEFRDRVIKA SLNHAHLVVS TSLQCYVFST
KNWNTPLIFD LKEGTVSLIL QAERHFLLVD GGGIYLHSYE GRFISSPKFP GMRTDILNAQ
TVSLSNDTIA IKDKADEKII FLFEASTGKP LGDGKLLSHK NEISEIALDQ KGLTNDRKIA
FIDKNRDLYI TSVKRFGKEE QIIKLGTMVH TLAWCDTCNI LCGIQDTRFT VWYYPNTIYV
DRDILPKTLY ERDASEYSKN PHIVSFVGNQ VTIRRADGSL VHISISPYPA ILHEYVSSSK
WEEAVRLCRF VKEQSMWACL AAMAVANRDM VTAEIAYAAV GEIDKVRYIN AIKDLPSRES
KMAHILMFSG NIQEAETVLL QAGLVYQAIQ ININLYNWER ALELAVKYKT HVDTVLAYRQ
KFLDTFGKQE TNKRYLQYAE GLQIDWEKIK AKIEMEITKE RDRSSSGQSS KSVGLKH
