IFT81_RAT
ID IFT81_RAT Reviewed; 675 AA.
AC P83829;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Intraflagellar transport protein 81 homolog;
DE AltName: Full=Carnitine deficiency-associated protein expressed in ventricle 1;
DE Short=CDV-1;
GN Name=Ift81; Synonyms=Cdv1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000312|EMBL:AAQ23131.1};
RN [1] {ECO:0000312|EMBL:AAQ23131.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAQ23131.1};
RA Zhou G., Wang J., Yu L., Zhao S.;
RT "Cloning of Rattus norvegicus carnitine deficiency-associated gene
RT expressed in ventricle 1.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the intraflagellar transport (IFT) complex B:
CC together with IFT74, forms a tubulin-binding module that specifically
CC mediates transport of tubulin within the cilium. Binds tubulin via its
CC CH (calponin-homology)-like region. Required for ciliogenesis. Required
CC for proper regulation of SHH signaling (By similarity). Plays an
CC important role during spermatogenesis by modulating the assembly and
CC elongation of the sperm flagella (By similarity).
CC {ECO:0000250|UniProtKB:O35594, ECO:0000250|UniProtKB:Q8WYA0}.
CC -!- SUBUNIT: Component of the IFT complex B, at least composed of IFT20,
CC IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74,
CC IFT80, IFT81, and IFT88. Interacts with IFT74; the interaction is
CC direct: within the IFT complex B, IFT74 and IFT81 mediate the transport
CC of tubulin within the cilium. Interacts with tubulin; the interaction
CC is direct (By similarity). Interacts with IFT57 and TTC30B (By
CC similarity). Interacts with RABL2/RABL2A; binding is equal in the
CC presence of GTP or GDP. Interacts with IFT88 (By similarity). Interacts
CC (via the IFT74/IFT81 heterodimer) with RABL2B (By similarity).
CC {ECO:0000250|UniProtKB:O35594, ECO:0000250|UniProtKB:Q8WYA0}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000250|UniProtKB:O35594}. Cytoplasm
CC {ECO:0000250|UniProtKB:O35594}.
CC -!- DOMAIN: The CH (calponin-homology)-like region shows high similarity to
CC a CH (calponin-homology) domain and mediated binding to the globular
CC domain of tubulin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the IFT81 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY345164; AAQ23131.1; -; mRNA.
DR RefSeq; NP_954551.1; NM_199120.1.
DR AlphaFoldDB; P83829; -.
DR SMR; P83829; -.
DR STRING; 10116.ENSRNOP00000061697; -.
DR jPOST; P83829; -.
DR PaxDb; P83829; -.
DR PRIDE; P83829; -.
DR GeneID; 373066; -.
DR KEGG; rno:373066; -.
DR UCSC; RGD:727862; rat.
DR CTD; 28981; -.
DR RGD; 727862; Ift81.
DR eggNOG; ENOG502QSBR; Eukaryota.
DR InParanoid; P83829; -.
DR OrthoDB; 301582at2759; -.
DR Reactome; R-RNO-5620924; Intraflagellar transport.
DR PRO; PR:P83829; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005814; C:centriole; ISO:RGD.
DR GO; GO:0005813; C:centrosome; ISO:RGD.
DR GO; GO:0036064; C:ciliary basal body; ISO:RGD.
DR GO; GO:0005929; C:cilium; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; ISS:UniProtKB.
DR GO; GO:0097225; C:sperm midpiece; ISO:RGD.
DR GO; GO:0097228; C:sperm principal piece; ISO:RGD.
DR GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR GO; GO:0035735; P:intraciliary transport involved in cilium assembly; ISS:UniProtKB.
DR GO; GO:0008589; P:regulation of smoothened signaling pathway; ISS:UniProtKB.
DR GO; GO:0120316; P:sperm flagellum assembly; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR Gene3D; 1.10.418.70; -; 1.
DR InterPro; IPR029600; IFT81.
DR InterPro; IPR041146; IFT81_CH.
DR InterPro; IPR043016; IFT81_N_sf.
DR PANTHER; PTHR15614; PTHR15614; 1.
DR Pfam; PF18383; IFT81_CH; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cell projection; Cilium; Cilium biogenesis/degradation;
KW Coiled coil; Cytoplasm; Differentiation; Phosphoprotein;
KW Reference proteome; Spermatogenesis.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8WYA0"
FT CHAIN 2..675
FT /note="Intraflagellar transport protein 81 homolog"
FT /id="PRO_0000084171"
FT REGION 2..121
FT /note="CH (calponin-homology)-like region"
FT /evidence="ECO:0000250"
FT COILED 132..258
FT /evidence="ECO:0000255"
FT COILED 306..389
FT /evidence="ECO:0000255"
FT COILED 433..453
FT /evidence="ECO:0000255"
FT COILED 509..627
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WYA0"
FT MOD_RES 61
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8WYA0"
SQ SEQUENCE 675 AA; 79029 MW; 08FD5F3D07864545 CRC64;
MSDQIKFIVD SLNKEPFKKN YNLITFDSLG PMQLLQVLND VLAEIDPKQD VDIREETPEQ
TAKRMLSLLG ILKYKPPGNA TDMSTFRQGL VIGSKPVIYP VLHWLLQRSS ELKKRAYLAR
FLIKLEVPSE FLQDETVADT NKQYEELMEA FKALHKECEQ LKTSGFSTAE IRRDISAMEE
EKDQLMKRVE RLKKRVETVQ NHQRMLKIAR QLRVEKEREE FLAQQKQEQK NQLFHAVQRL
QRVQNQLKSM RHAAADAKPE SLMKRLEEEI KFNSYMVTEK FPKELESKKK ELHFLQKVVS
EPAMGHSDLL ELESKVNEVN AEINQLIEKK MMRNEPIEGK LSLYRQQASI ISRKKEAKAE
ELQETKEKLA SLEREVSVKT NQTREFDGTE VLKGDEFKRY VSKLRSKSTV FKKKHQIIAE
FKAEFGLLQR TEELLKQRQE TIQHQLRTIE EKKGISGYSY TQEELERVSA LKSEVDEMKG
RTLDDMSEMV KKLNSLVSEK KSALAPVIKE LRQLRQKCQL TQECDEKRTQ YDSCAAGLES
NRSKLEQEVR GLREECLQEE SKYHYTNCMI KNLEVQLRRA TDEMKAYVSS DQQEKRKAIR
EQYTKNITEQ ENLGKKLREK QKAVRESHGP NMKQAKMWRD LEQLMECKKQ CFLKQQSPAS
IGQVIQEGGE DRLVL
