IFTAP_MOUSE
ID IFTAP_MOUSE Reviewed; 244 AA.
AC Q9CQI4; A2AVN5; A2AVN6; Q8C2H0; Q8R186;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Intraflagellar transport-associated protein;
DE AltName: Full=Protein HEPIS;
DE AltName: Full=Protein NWC;
GN Name=Iftap; Synonyms=Nwc;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=15971274; DOI=10.1002/eji.200526225;
RA Cebrat M.A., Miazek A., Kisielow P.;
RT "Identification of a third evolutionarily conserved gene within RAG locus
RT and its RAG1 dependent and independent regulation.";
RL Eur. J. Immunol. 35:2230-2238(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, Spinal ganglion, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=30476139; DOI=10.1093/jb/mvy100;
RA Takahara M., Kunii M., Nakamura K., Harada A., Hirano T., Katoh Y.,
RA Nakayama K.;
RT "C11ORF74 interacts with the IFT-A complex and participates in ciliary
RT BBSome localization.";
RL J. Biochem. 165:257-267(2019).
CC -!- FUNCTION: Seems to play a role in ciliary BBSome localization, maybe
CC through interaction with IFT-A complex. {ECO:0000250|UniProtKB:Q86VG3}.
CC -!- SUBUNIT: Interacts with IFT122; the interaction associates IFTAP with
CC IFT-A complex. {ECO:0000250|UniProtKB:Q86VG3}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:15971274}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice show no obvious anatomical
CC abnormalities associated with ciliary dysfunction.
CC {ECO:0000269|PubMed:30476139}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM20885.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAM20886.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ112091; AAZ17507.1; -; mRNA.
DR EMBL; DQ112092; AAZ17508.1; -; mRNA.
DR EMBL; AK010820; BAB27203.1; -; mRNA.
DR EMBL; AK020980; BAB32268.1; -; mRNA.
DR EMBL; AK051679; BAC34718.1; -; mRNA.
DR EMBL; AK088637; BAC40469.1; -; mRNA.
DR EMBL; AL929569; CAM20884.1; -; Genomic_DNA.
DR EMBL; AL929569; CAM20885.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL929569; CAM20886.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC025075; AAH25075.1; -; mRNA.
DR CCDS; CCDS16461.1; -.
DR RefSeq; NP_080868.1; NM_026592.3.
DR AlphaFoldDB; Q9CQI4; -.
DR BioGRID; 212696; 1.
DR STRING; 10090.ENSMUSP00000106862; -.
DR iPTMnet; Q9CQI4; -.
DR PhosphoSitePlus; Q9CQI4; -.
DR jPOST; Q9CQI4; -.
DR MaxQB; Q9CQI4; -.
DR PaxDb; Q9CQI4; -.
DR PRIDE; Q9CQI4; -.
DR ProteomicsDB; 285452; -.
DR Antibodypedia; 26025; 123 antibodies from 20 providers.
DR DNASU; 68170; -.
DR Ensembl; ENSMUST00000090513; ENSMUSP00000087999; ENSMUSG00000027165.
DR Ensembl; ENSMUST00000111231; ENSMUSP00000106862; ENSMUSG00000027165.
DR Ensembl; ENSMUST00000160037; ENSMUSP00000123987; ENSMUSG00000027165.
DR Ensembl; ENSMUST00000160722; ENSMUSP00000124783; ENSMUSG00000027165.
DR GeneID; 68170; -.
DR KEGG; mmu:68170; -.
DR UCSC; uc008lhg.1; mouse.
DR CTD; 119710; -.
DR MGI; MGI:1915420; Iftap.
DR VEuPathDB; HostDB:ENSMUSG00000027165; -.
DR eggNOG; ENOG502S1X7; Eukaryota.
DR GeneTree; ENSGT00390000013149; -.
DR HOGENOM; CLU_077972_0_0_1; -.
DR InParanoid; Q9CQI4; -.
DR OMA; FTHKNEP; -.
DR OrthoDB; 1316206at2759; -.
DR PhylomeDB; Q9CQI4; -.
DR TreeFam; TF335968; -.
DR BioGRID-ORCS; 68170; 0 hits in 71 CRISPR screens.
DR ChiTaRS; B230118H07Rik; mouse.
DR PRO; PR:Q9CQI4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9CQI4; protein.
DR Bgee; ENSMUSG00000027165; Expressed in saccule of membranous labyrinth and 249 other tissues.
DR ExpressionAtlas; Q9CQI4; baseline and differential.
DR Genevisible; Q9CQI4; MM.
DR GO; GO:0097731; C:9+0 non-motile cilium; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0120160; F:intraciliary transport particle A binding; IDA:MGI.
DR GO; GO:0007340; P:acrosome reaction; IMP:MGI.
DR GO; GO:0009566; P:fertilization; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR InterPro; IPR040028; IFTAP.
DR PANTHER; PTHR35543; PTHR35543; 1.
DR Pfam; PF17722; IFTAP; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..244
FT /note="Intraflagellar transport-associated protein"
FT /id="PRO_0000288855"
FT MOD_RES 58
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86VG3"
FT MOD_RES 235
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 77
FT /note="R -> C (in Ref. 4; AAH25075)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="S -> T (in Ref. 4; AAH25075)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 244 AA; 27654 MW; 89C7DC7152BD782B CRC64;
MLAQIPRPEI MDEDQLMKEV LDKFVNCHEQ TYEEFLSTFT HLSKEDNVAK WGAHGTDSSE
NIFSTVKWNH KTESNGRHFR NTSIFLHTSS EHSEEDQIVI DEGHKVGSSF QGDMTRAGKV
KVDNFLDLED VDMEEESKPQ MNKDCLLLPG EVEQDISTSV PSYDPSVDLP LTSEVRPKPA
VGRTQKQTEE ILGDEVQPFF LDEEFDYDNV TLTPKFTAGE IATIQELSKQ KRVGSGTNVC
ELHD
