IFTNT_MYCTU
ID IFTNT_MYCTU Reviewed; 176 AA.
AC O05443; F2GDR3; I6YDB2; Q7D4M5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Immunity factor for TNT {ECO:0000303|PubMed:26237511};
DE Short=IFT {ECO:0000303|PubMed:26237511};
DE AltName: Full=Tuberculosis necrotizing toxin antitoxin {ECO:0000305};
DE Short=TNT antitoxin {ECO:0000305};
GN OrderedLocusNames=Rv3902c {ECO:0000312|EMBL:CCP46731.1},
GN LH57_21250 {ECO:0000312|EMBL:AIR16696.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4] {ECO:0007744|PDB:4QLP}
RP X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF 2-176 IN COMPLEX WITH TNT,
RP FUNCTION, AND SUBUNIT.
RX PubMed=26237511; DOI=10.1038/nsmb.3064;
RA Sun J., Siroy A., Lokareddy R.K., Speer A., Doornbos K.S., Cingolani G.,
RA Niederweis M.;
RT "The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD.";
RL Nat. Struct. Mol. Biol. 22:672-678(2015).
CC -!- FUNCTION: Antitoxin for tuberculosis necrotizing toxin (TNT). Acts by
CC binding directly to TNT, which inhibits NAD(+) glycohydrolase activity
CC of TNT and protects M.tuberculosis from self-poisoning.
CC {ECO:0000269|PubMed:26237511}.
CC -!- SUBUNIT: Interacts with the tuberculosis necrotizing toxin (TNT), the
CC C-terminal domain of the outer membrane channel protein CpnT.
CC {ECO:0000269|PubMed:26237511}.
CC -!- INTERACTION:
CC O05443; O05442: cpnT; NbExp=5; IntAct=EBI-16167151, EBI-16167127;
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DR EMBL; CP009480; AIR16696.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP46731.1; -; Genomic_DNA.
DR RefSeq; NP_218419.1; NC_000962.3.
DR RefSeq; WP_003400086.1; NZ_NVQJ01000005.1.
DR PDB; 4QLP; X-ray; 1.10 A; A=2-176.
DR PDBsum; 4QLP; -.
DR AlphaFoldDB; O05443; -.
DR SMR; O05443; -.
DR IntAct; O05443; 1.
DR STRING; 83332.Rv3902c; -.
DR PaxDb; O05443; -.
DR DNASU; 886236; -.
DR GeneID; 886236; -.
DR KEGG; mtu:Rv3902c; -.
DR PATRIC; fig|83332.111.peg.4346; -.
DR TubercuList; Rv3902c; -.
DR eggNOG; ENOG502ZPGV; Bacteria.
DR HOGENOM; CLU_1546538_0_0_11; -.
DR OMA; YEGVERF; -.
DR Proteomes; UP000001584; Chromosome.
DR InterPro; IPR028953; Imm_IFT-like.
DR Pfam; PF15598; Imm61; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..176
FT /note="Immunity factor for TNT"
FT /id="PRO_0000437788"
FT HELIX 9..17
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 21..23
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 28..30
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 38..40
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 41..49
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 52..59
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 65..72
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 73..91
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 103..105
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 111..117
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:4QLP"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 138..149
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 152..160
FT /evidence="ECO:0007829|PDB:4QLP"
FT TURN 165..167
FT /evidence="ECO:0007829|PDB:4QLP"
FT HELIX 171..173
FT /evidence="ECO:0007829|PDB:4QLP"
SQ SEQUENCE 176 AA; 19822 MW; 69925EC3709502EB CRC64;
MTIGVDLSTD LQDWIRLSGM NMIQGSETND GRTILWNKGG EVRYFIDRLA GWYVITSSDR
MSREGYEFAA ASMSVIEKYL YGYFGGSVRS ERELPAIRAP FQPEELMPEY SIGTMTFAGR
QRDTLIDSSG TVVAITAADR LVELSHYLDV SVNVIKDSFL DSEGKPLFTL WKDYKG
