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IF_RAT
ID   IF_RAT                  Reviewed;         417 AA.
AC   P17267; O35801;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Cobalamin binding intrinsic factor {ECO:0000305};
DE   AltName: Full=Gastric intrinsic factor {ECO:0000305};
DE   AltName: Full=Intrinsic factor;
DE            Short=IF;
DE            Short=INF;
DE   Flags: Precursor;
GN   Name=Cblif {ECO:0000312|RGD:62084}; Synonyms=Gif {ECO:0000312|RGD:62084};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 262-276 AND 384-392.
RX   PubMed=3422425; DOI=10.1073/pnas.85.1.46;
RA   Dieckgraefe B.K., Seetharam B., Banaszak L., Leykam J.F., Alpers D.H.;
RT   "Isolation and structural characterization of a cDNA clone encoding rat
RT   gastric intrinsic factor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:46-50(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-170.
RC   STRAIN=Sprague-Dawley; TISSUE=Liver;
RX   PubMed=7490275; DOI=10.1093/oxfordjournals.jbchem.a124859;
RA   Maeda M., Asahara S., Nishi T., Mushiake S., Oka T., Shimada S., Chiba T.,
RA   Tohyama M., Futai M.;
RT   "The rat intrinsic factor gene: its 5'-upstream region and chief cell-
RT   specific transcription.";
RL   J. Biochem. 117:1305-1311(1995).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Promotes absorption of the essential vitamin cobalamin (Cbl)
CC       in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex
CC       is internalized via receptor-mediated endocytosis (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with CUBN (via CUB domains). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Gastric mucosa.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the eukaryotic cobalamin transport proteins
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA41361.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAA08140.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; J03577; AAA41361.1; ALT_INIT; mRNA.
DR   EMBL; D45200; BAA08140.1; ALT_INIT; Genomic_DNA.
DR   PIR; A34003; A34003.
DR   RefSeq; NP_058858.1; NM_017162.3.
DR   AlphaFoldDB; P17267; -.
DR   SMR; P17267; -.
DR   STRING; 10116.ENSRNOP00000028507; -.
DR   GlyGen; P17267; 4 sites.
DR   iPTMnet; P17267; -.
DR   PhosphoSitePlus; P17267; -.
DR   PaxDb; P17267; -.
DR   GeneID; 29319; -.
DR   KEGG; rno:29319; -.
DR   UCSC; RGD:62084; rat.
DR   CTD; 2694; -.
DR   RGD; 62084; Cblif.
DR   eggNOG; ENOG502RXIA; Eukaryota.
DR   InParanoid; P17267; -.
DR   OrthoDB; 1233171at2759; -.
DR   PhylomeDB; P17267; -.
DR   TreeFam; TF333092; -.
DR   Reactome; R-RNO-9758881; Uptake of dietary cobalamins into enterocytes.
DR   PRO; PR:P17267; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0016324; C:apical plasma membrane; ISO:RGD.
DR   GO; GO:0005768; C:endosome; ISO:RGD.
DR   GO; GO:0098591; C:external side of apical plasma membrane; ISO:RGD.
DR   GO; GO:0005615; C:extracellular space; ISO:RGD.
DR   GO; GO:0005902; C:microvillus; ISO:RGD.
DR   GO; GO:0140355; F:cargo receptor ligand activity; ISO:RGD.
DR   GO; GO:0031419; F:cobalamin binding; IDA:RGD.
DR   GO; GO:0019842; F:vitamin binding; TAS:RGD.
DR   GO; GO:0009235; P:cobalamin metabolic process; IDA:RGD.
DR   GO; GO:0015889; P:cobalamin transport; ISO:RGD.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR002157; Cbl-bd_prot.
DR   Pfam; PF01122; Cobalamin_bind; 1.
DR   PROSITE; PS00468; COBALAMIN_BINDING; 1.
PE   1: Evidence at protein level;
KW   Cobalt; Cobalt transport; Direct protein sequencing; Disulfide bond;
KW   Glycoprotein; Ion transport; Phosphoprotein; Reference proteome; Secreted;
KW   Signal; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000305"
FT   CHAIN           19..417
FT                   /note="Cobalamin binding intrinsic factor"
FT                   /id="PRO_0000005560"
FT   BINDING         171
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT                   /evidence="ECO:0000250"
FT   BINDING         222
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT                   /evidence="ECO:0000250"
FT   BINDING         270
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT                   /evidence="ECO:0000250"
FT   BINDING         365..370
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT                   /evidence="ECO:0000250"
FT   BINDING         386..395
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         191
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   CARBOHYD        209
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        311
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        330
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        413
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        26..246
FT                   /evidence="ECO:0000250"
FT   DISULFID        103..288
FT                   /evidence="ECO:0000250"
FT   DISULFID        143..182
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   417 AA;  45956 MW;  881BF5B2C77563C3 CRC64;
     MAWLSFYLLN VLWAVAGTST RAQRSCSVPP DQQPWVNGLQ LLMENSVTES DLPNPSILIA
     MNLASTYNLE AQKLLTYQLM ASDSADLTNG QLALTIMALT SSCRDPGSKV SILQKNMESW
     TPSNLGAESS SFYGPALAIL ALCQKNSEAT LPIAVRFAKT LMMESSPFSV DTGAVATLAL
     TCMYNRIPVG SQENYRDLFG QALKVIVDNI SLRIKADGII GDIYSTGLAM QALSVTPEQP
     TKEWDCEKTM YTILKEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD
     YPTPVPTSIS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA QRRNHMFKFE
     TTMTSWGLIV SSINNIAENV KHKTYWEFLS GKTPLGEGVA YYIPFNYEHI TANFTQY
 
 
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