ID   API1_MYCTU              Reviewed;          84 AA.
AC   O69622;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Apoptosis inhibitor Rv3654c {ECO:0000305|PubMed:20454556};
DE   Flags: Precursor;
GN   OrderedLocusNames=Rv3654c {ECO:0000312|EMBL:CCP46477.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HUMAN PSF.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20454556; DOI=10.1371/journal.pone.0010474;
RA   Danelishvili L., Yamazaki Y., Selker J., Bermudez L.E.;
RT   "Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c proteins
RT   participate in the suppression of macrophage apoptosis.";
RL   PLoS ONE 5:E10474-E10474(2010).
CC   -!- FUNCTION: Effector protein that participates in the suppression of
CC       macrophage apoptosis by blocking the extrinsic pathway. Recognizes the
CC       host polypyrimidine tract binding protein-associated splicing factor
CC       (PSF), which probably leads to its cleavage, diminishing the level of
CC       caspase-8 in macrophages. {ECO:0000269|PubMed:20454556}.
CC   -!- SUBUNIT: Interacts with human polypyrimidine tract binding protein-
CC       associated splicing factor (PSF). {ECO:0000269|PubMed:20454556}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20454556}. Host
CC       cytoplasm {ECO:0000269|PubMed:20454556}. Note=Is secreted into the
CC       macrophage cytoplasm. Might be secreted via a type IV pili apparatus
CC       that seems to be encoded in the same operon.
CC       {ECO:0000269|PubMed:20454556}.
CC   -!- INDUCTION: Component of the Rv3654c-Rv3660c operon that is highly up-
CC       regulated during M.tuberculosis infection of macrophages.
CC       {ECO:0000269|PubMed:20454556}.
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DR   EMBL; AL123456; CCP46477.1; -; Genomic_DNA.
DR   AlphaFoldDB; O69622; -.
DR   STRING; 83332.Rv3654c; -.
DR   PaxDb; O69622; -.
DR   TubercuList; Rv3654c; -.
DR   OMA; GWWSATH; -.
DR   Reactome; R-HSA-9635465; Suppression of apoptosis.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033668; P:negative regulation by symbiont of host apoptotic process; IDA:MTBBASE.
DR   InterPro; IPR021202; Rv3654c-like.
DR   TIGRFAMs; TIGR03816; tadE_like_DECH; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..39
FT                   /evidence="ECO:0000255"
FT   CHAIN           40..84
FT                   /note="Apoptosis inhibitor Rv3654c"
FT                   /id="PRO_0000438183"
SQ   SEQUENCE   84 AA;  8359 MW;  BC16A908A438F4BF CRC64;
     MVARHRAQAA ADLASLAAAA RLPSGLAAAC ARATLVARAM RVEHAQCRVV DLDVVVTVEV
     AVAFAGVATA TARAGPAKVP TTPG