ID   API2_MYCTU              Reviewed;         125 AA.
AC   O69623;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Apoptosis inhibitor Rv3655c {ECO:0000305|PubMed:20454556};
DE   Flags: Precursor;
GN   OrderedLocusNames=Rv3655c {ECO:0000312|EMBL:CCP46478.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HUMAN
RP   RNF213.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20454556; DOI=10.1371/journal.pone.0010474;
RA   Danelishvili L., Yamazaki Y., Selker J., Bermudez L.E.;
RT   "Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c proteins
RT   participate in the suppression of macrophage apoptosis.";
RL   PLoS ONE 5:E10474-E10474(2010).
CC   -!- FUNCTION: Effector protein that participates in the suppression of
CC       macrophage apoptosis by blocking the extrinsic pathway. Interferes with
CC       caspase-8 activation and binds to the host E3 ubiquitin-protein ligase
CC       RNF213, whose fusion partners have anti-apoptotic function.
CC       {ECO:0000269|PubMed:20454556}.
CC   -!- SUBUNIT: Interacts with human E3 ubiquitin-protein ligase RNF213.
CC       {ECO:0000269|PubMed:20454556}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20454556}. Host
CC       cytoplasm {ECO:0000269|PubMed:20454556}. Note=Is secreted into the
CC       macrophage cytoplasm. Might be secreted via a type IV pili apparatus
CC       that seems to be encoded in the same operon.
CC       {ECO:0000269|PubMed:20454556}.
CC   -!- INDUCTION: Component of the Rv3654c-Rv3660c operon that is highly up-
CC       regulated during M.tuberculosis infection of macrophages.
CC       {ECO:0000269|PubMed:20454556}.
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DR   EMBL; AL123456; CCP46478.1; -; Genomic_DNA.
DR   RefSeq; NP_218172.1; NC_000962.3.
DR   RefSeq; WP_010886180.1; NC_000962.3.
DR   AlphaFoldDB; O69623; -.
DR   STRING; 83332.Rv3655c; -.
DR   PaxDb; O69623; -.
DR   GeneID; 885614; -.
DR   KEGG; mtu:Rv3655c; -.
DR   PATRIC; fig|83332.12.peg.4075; -.
DR   TubercuList; Rv3655c; -.
DR   eggNOG; ENOG5033A2X; Bacteria.
DR   OMA; GICALSM; -.
DR   Reactome; R-HSA-9635465; Suppression of apoptosis.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033668; P:negative regulation by symbiont of host apoptotic process; IDA:MTBBASE.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..125
FT                   /note="Apoptosis inhibitor Rv3655c"
FT                   /id="PRO_0000438184"
SQ   SEQUENCE   125 AA;  13007 MW;  3847D59540748EC7 CRC64;
     MEAALAIATL VLVLVLCLAG VTAVSMQVRC IDAAREAARL AARGDVRSAT DVARSIAPRA
     ALVQVHRDGE FVVATVTAHS NLLPTLDIAA RAISVAEPGS TAARPPCLPS RWSRCCCASP
     VRVHI