ID   IHFA_RHOCA              Reviewed;         100 AA.
AC   P30787;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Integration host factor subunit alpha;
DE            Short=IHF-alpha;
GN   Name=ihfA; Synonyms=himA;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=1961742; DOI=10.1073/pnas.88.23.10749;
RA   Toussaint B., Bosc C., Richaud P., Colbeau A., Vignais P.M.;
RT   "A mutation in a Rhodobacter capsulatus gene encoding an integration host
RT   factor-like protein impairs in vivo hydrogenase expression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:10749-10753(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-15 (MUTANT IR4).
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=8407826; DOI=10.1128/jb.175.20.6499-6504.1993;
RA   Toussaint B., Delic-Attree I., de Sury D'Aspremont R., David L., Vincon M.,
RA   Vignais P.M.;
RT   "Purification of the integration host factor homolog of Rhodobacter
RT   capsulatus: cloning and sequencing of the hip gene, which encodes the beta
RT   subunit.";
RL   J. Bacteriol. 175:6499-6504(1993).
CC   -!- FUNCTION: This protein is one of the two subunits of integration host
CC       factor, a specific DNA-binding protein that functions in genetic
CC       recombination as well as in transcriptional and translational control.
CC       Involved in hydrogenase gene expression.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- SIMILARITY: Belongs to the bacterial histone-like protein family.
CC       {ECO:0000305}.
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DR   EMBL; M84030; AAA26128.1; -; Genomic_DNA.
DR   PIR; D41608; D41608.
DR   AlphaFoldDB; P30787; -.
DR   SMR; P30787; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd13835; IHF_A; 1.
DR   Gene3D; 4.10.520.10; -; 1.
DR   HAMAP; MF_00380; IHF_alpha; 1.
DR   InterPro; IPR000119; Hist_DNA-bd.
DR   InterPro; IPR020816; Histone-like_DNA-bd_CS.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR005684; IHF_alpha.
DR   PANTHER; PTHR33175; PTHR33175; 1.
DR   Pfam; PF00216; Bac_DNA_binding; 1.
DR   PRINTS; PR01727; DNABINDINGHU.
DR   SMART; SM00411; BHL; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
DR   TIGRFAMs; TIGR00987; himA; 1.
DR   PROSITE; PS00045; HISTONE_LIKE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA recombination; DNA-binding; Transcription;
KW   Transcription regulation; Translation regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8407826"
FT   CHAIN           2..100
FT                   /note="Integration host factor subunit alpha"
FT                   /id="PRO_0000105024"
FT   MUTAGEN         8
FT                   /note="R->C: In IR4; altered DNA-binding specificity."
SQ   SEQUENCE   100 AA;  11306 MW;  A336453F99FF5873 CRC64;
     MSEKTLTRMD LSEAVFREVG LSRNESAQLV ETVLQHMSDA LVRGETVKIS SFGTFSVRDK
     TSRMGRNPKT GEEVPISPRR VLSFRPSHLM KDRVAERNAK