ID   APIM_APIME              Reviewed;          78 AA.
AC   Q8ISL8;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Apisimin {ECO:0000303|PubMed:12297291};
DE   AltName: Full=Apisin subunit Apisimin {ECO:0000303|PubMed:27721892};
DE   AltName: Full=Royal jelly protein RJP54 {ECO:0000303|PubMed:12297291};
DE   Flags: Precursor;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 25-31,
RP   SUBCELLULAR LOCATION, SUBUNIT, AND MASS SPECTROMETRY.
RX   PubMed=12297291; DOI=10.1016/s0014-5793(02)03272-6;
RA   Bilikova K., Hanes J., Nordhoff E., Saenger W., Klaudiny J., Simuth J.;
RT   "Apisimin, a new serine-valine-rich peptide from honeybee (Apis mellifera
RT   L.) royal jelly: purification and molecular characterization.";
RL   FEBS Lett. 528:125-129(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DH4;
RG   Honey bee genome project;
RA   Zhang L., Deng J., Wu Y.-Q., Kovar C., Aqrawi P., Bandaranaike D.,
RA   Blankenburg K., Chen D., Denson S., Dinh H., Firestine M., Gross S.,
RA   Han Y., Hernandez B., Holder M., Jackson L., Javaid M., Jing C., Jones J.,
RA   Joshi V., Kamau G., Korchina V., Lee S., Lorensuhewa L., Mata R.,
RA   Mathew T., Mims S., Ngo R., Nguyen L., Okwuonu G., Ongeri F., Osuji N.,
RA   Pham C., Puazo M., Qu C., Quiroz J., Raj R., Rio Deiros D., Santibanez J.,
RA   Scheel M., Scherer S., Vee V., Wang M., Xin Y., Richards S., Reid J.G.,
RA   Newsham I., Worley K.C., Muzny D.M., Gibbs R.;
RL   Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   TISSUE SPECIFICITY.
RA   Klaudiny J., Kulifajova J., Crailsheim K., Simuth J.;
RT   "New approach to the study of division of labour in the honeybee colony
RT   (Apis mellifera L).";
RL   Apidologie 32:69-80(1994).
RN   [4]
RP   SUBUNIT.
RX   PubMed=27721892; DOI=10.1155/2016/5040528;
RA   Furusawa T., Arai Y., Kato K., Ichihara K.;
RT   "Quantitative analysis of apisin, a major protein unique to royal jelly.";
RL   Evid. Based Complement Alternat. Med. 2016:5040528-5040528(2016).
CC   -!- FUNCTION: May play an important role in honeybee nutrition. However,
CC       its role could not only be associated with larval development, because
CC       high level of mRNA is found in both forager and nurse honeybees
CC       (PubMed:12297291). No antimicrobial activity (tested on E.coli,
CC       B.subtilis or Paenibacillus l. larvae) has been observed
CC       (PubMed:12297291). {ECO:0000269|PubMed:12297291}.
CC   -!- SUBUNIT: Occurs as monomer and a variety of oligomers
CC       (PubMed:12297291). Interacts with apisimin to form the heterooligomer
CC       apisin (350 kDa) (PubMed:27721892). {ECO:0000269|PubMed:12297291,
CC       ECO:0000269|PubMed:27721892}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12297291}.
CC       Note=Royal jelly. {ECO:0000269|PubMed:12297291}.
CC   -!- TISSUE SPECIFICITY: High level of apisimin-mRNA is found in the heads
CC       of both nurse and forager honeybees. {ECO:0000269|Ref.3}.
CC   -!- DEVELOPMENTAL STAGE: Is synthesized during the whole life span of
CC       honeybee. {ECO:0000305|PubMed:12297291}.
CC   -!- MASS SPECTROMETRY: Mass=5540.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12297291};
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAQ16586.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AY055108; AAL15544.1; -; mRNA.
DR   EMBL; AY340960; AAQ16586.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AADG06006130; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001011582.1; NM_001011582.1.
DR   PDB; 5YYL; X-ray; 2.65 A; C/D=1-78.
DR   PDB; 7ASD; EM; 3.50 A; AB/BB/CB/DB/EB/FB/GB/HB=1-78.
DR   PDBsum; 5YYL; -.
DR   PDBsum; 7ASD; -.
DR   AlphaFoldDB; Q8ISL8; -.
DR   SMR; Q8ISL8; -.
DR   STRING; 7460.GB53576-PA; -.
DR   PaxDb; Q8ISL8; -.
DR   EnsemblMetazoa; NM_001011582; NP_001011582; LOC406093.
DR   GeneID; 406093; -.
DR   KEGG; ame:406093; -.
DR   HOGENOM; CLU_2624048_0_0_1; -.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:12297291"
FT   CHAIN           25..78
FT                   /note="Apisimin"
FT                   /evidence="ECO:0000269|PubMed:12297291"
FT                   /id="PRO_5014588991"
FT   HELIX           38..51
FT                   /evidence="ECO:0007829|PDB:5YYL"
FT   HELIX           57..73
FT                   /evidence="ECO:0007829|PDB:5YYL"
SQ   SEQUENCE   78 AA;  7946 MW;  C572397FB420B8B9 CRC64;
     MSKIVAVVVL AAFCVAMLVS DVSAKTSISV KGESNVDVVS QINSLVSSIV SGANVSAVLL
     AQTLVNILQI LIDANVFA