ID   API_DIRIM               Reviewed;         234 AA.
AC   Q27384;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Pepsin inhibitor Dit33;
DE   Flags: Precursor;
GN   Name=DIT33;
OS   Dirofilaria immitis (Canine heartworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Dirofilaria.
OX   NCBI_TaxID=6287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8728997; DOI=10.1017/s0031182000065859;
RA   Hong X.Q., Mejia J.S., Kumar S., Perler F.B., Carlow C.K.S.;
RT   "Cloning and expression of DiT33 from Dirofilaria immitis: a specific and
RT   early marker of heartworm infection.";
RL   Parasitology 112:331-338(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Wisnewski N., Frank G.R., Grieve R.B.;
RT   "Molecular cloning of a Dirofilaria immitis aspartyl protease inhibitor
RT   homologue.";
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Aspartyl protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I33 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U24180; AAC47103.1; -; mRNA.
DR   EMBL; U31450; AAA70419.1; -; mRNA.
DR   AlphaFoldDB; Q27384; -.
DR   MEROPS; I33.002; -.
DR   PRIDE; Q27384; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019828; F:aspartic-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1120.50; -; 2.
DR   InterPro; IPR010480; Pepsin-I3.
DR   InterPro; IPR038412; Pepsin-I3_sf.
DR   Pfam; PF06394; Pepsin-I3; 2.
PE   2: Evidence at transcript level;
KW   Aspartic protease inhibitor; Disulfide bond; Protease inhibitor; Secreted;
KW   Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..234
FT                   /note="Pepsin inhibitor Dit33"
FT                   /id="PRO_0000002396"
FT   REGION          200..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        135..230
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   234 AA;  26433 MW;  8C44F6A50E6322A7 CRC64;
     MKILFCFVLL AIAALRASVI NRHNKRFAGF SVAGIGGTAG CVVVDNKLFA NSFYLRDLTT
     EEQRELAQYV EDSNQYKEEV KTSLEERRKG WQLARHGEKD AKVLSSLAEK KFPKPPKKPS
     FCSAGDTTQY YFDGCMVQNN KIYVGRMYVR DLTSDEINQL KTFDAKMTAY QKYLSSSIQQ
     QVDSLFGDKS NLFNLFTDTR HETSSQPSDA TTISTTTQAP VEPPETPHFC IAIY