ID   API_OSTOS               Reviewed;         227 AA.
AC   Q95PP1;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Aspartyl protease inhibitor;
DE   Flags: Precursor;
GN   Name=API;
OS   Ostertagia ostertagi (Brown stomach worm) (Strongylus ostertagi).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Strongylida;
OC   Trichostrongyloidea; Haemonchidae; Ostertagia.
OX   NCBI_TaxID=6317;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   De Maere V., Vercauteren I., Claerebout E., Vercruysse J.;
RT   "Identification of aspartyl protease inhibitor of Ostertagia ostertagi.";
RL   Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Aspartyl protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I33 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ418047; CAD10783.1; -; mRNA.
DR   AlphaFoldDB; Q95PP1; -.
DR   SMR; Q95PP1; -.
DR   MEROPS; I33.002; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019828; F:aspartic-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1120.50; -; 2.
DR   InterPro; IPR010480; Pepsin-I3.
DR   InterPro; IPR038412; Pepsin-I3_sf.
DR   Pfam; PF06394; Pepsin-I3; 2.
PE   2: Evidence at transcript level;
KW   Aspartic protease inhibitor; Disulfide bond; Protease inhibitor; Secreted;
KW   Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..227
FT                   /note="Aspartyl protease inhibitor"
FT                   /id="PRO_0000002398"
FT   REGION          88..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        88..111
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        131..223
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   227 AA;  24958 MW;  BC744D97B2E0A5E4 CRC64;
     MKLVVLCVLC GIALAAPRQK RLTVGTIAVT GGVGGSTGCV VTGNVLYANG FRLRELNPSE
     QQELVNYEKQ VADYKAAVKQ ALKERQESLK SRMAGKKEKA VTPKEEDLPK APQKPSFCTE
     DDTTQFYFDG CMVQGNKVYV GNTFARDLDQ NEIQELKEFE KKQTVYQEYV QKQIQAQVSN
     LFGGADFFSS FFNGGSEKGS STTTVAPVLP EDAPEQPAGP NFCTRIY