APL25_ORYSJ
ID APL25_ORYSJ Reviewed; 460 AA.
AC A0A0N7KJT8; B9FCV0; Q7XT53;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=APETALA2-like protein 5 {ECO:0000303|PubMed:28066457};
DE AltName: Full=Protein SHATTERING ABORTION 1 {ECO:0000303|PubMed:22408071};
GN Name=AP2-5 {ECO:0000303|PubMed:28066457};
GN Synonyms=SHAT1 {ECO:0000303|PubMed:22408071};
GN OrderedLocusNames=LOC_Os04g55560 {ECO:0000305}, Os04g0649100 {ECO:0000305};
GN ORFNames=OSJNBa0010D21.13 {ECO:0000312|EMBL:CAE01667.2},
GN OSNPB_040649100 {ECO:0000312|EMBL:BAS91337.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Huayong;
RX PubMed=22408071; DOI=10.1105/tpc.111.094383;
RA Zhou Y., Lu D., Li C., Luo J., Zhu B.-F., Zhu J., Shangguan Y., Wang Z.,
RA Sang T., Zhou B., Han B.;
RT "Genetic control of seed shattering in rice by the APETALA2 transcription
RT factor shattering abortion1.";
RL Plant Cell 24:1034-1048(2012).
RN [6]
RP INDUCTION BY SH5 AND COLD, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Dongjin;
RX PubMed=24923192; DOI=10.1111/tpj.12581;
RA Yoon J., Cho L.-H., Kim S.L., Choi H., Koh H.-J., An G.;
RT "The BEL1-type homeobox gene SH5 induces seed shattering by enhancing
RT abscission-zone development and inhibiting lignin biosynthesis.";
RL Plant J. 79:717-728(2014).
RN [7]
RP FUNCTION, AND REPRESSION BY MIR172.
RX PubMed=26631749; DOI=10.1073/pnas.1521949112;
RA Wang L., Sun S., Jin J., Fu D., Yang X., Weng X., Xu C., Li X., Xiao J.,
RA Zhang Q.;
RT "Coordinated regulation of vegetative and reproductive branching in rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:15504-15509(2015).
RN [8]
RP FUNCTION, REPRESSION BY MIR172, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Zhonghua 11;
RX PubMed=28066457; DOI=10.3389/fpls.2016.01891;
RA Dai Z., Wang J., Zhu M., Miao X., Shi Z.;
RT "OsMADS1 represses microRNA172 in elongation of palea/lemma development in
RT rice.";
RL Front. Plant Sci. 7:1891-1891(2016).
CC -!- FUNCTION: Transcription factor (PubMed:22408071). Involved in spikelet
CC transition and development (Probable) (PubMed:22408071). Prevents lemma
CC and palea elongation as well as grain growth (PubMed:28066457,
CC PubMed:22408071). Required for seed shattering through specifying
CC abscission zone (AZ) development (PubMed:22408071).
CC {ECO:0000269|PubMed:22408071, ECO:0000269|PubMed:28066457,
CC ECO:0000305|PubMed:26631749}.
CC -!- SUBUNIT: May form homodimer. {ECO:0000250|UniProtKB:P47927}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366,
CC ECO:0000269|PubMed:22408071}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, leaves, stems, nodes,
CC sheaths, panicles and young spikelets (PubMed:22408071). Accumulates in
CC the spikelet abscission zone (AZ) via a positive regulation by the
CC transcription factors SH4 and SH5 (PubMed:22408071, PubMed:24923192).
CC {ECO:0000269|PubMed:22408071, ECO:0000269|PubMed:24923192}.
CC -!- DEVELOPMENTAL STAGE: In developing panicles, highly expressed in the
CC spikelet abscission zone (AZ) and the inner floral organs of 2 mm long
CC spikelets. Later present at weak levels in the apiculus as well as in
CC the palea and lemma to progressively fades out in 8 mm long spikelets.
CC {ECO:0000269|PubMed:22408071}.
CC -!- INDUCTION: Target of miR172 microRNA mediated cleavage, particularly
CC during floral organ development (Probable). Induced by SH5 in spikelets
CC abscission zones (AZ). Triggered by cold stress (PubMed:24923192).
CC {ECO:0000269|PubMed:24923192, ECO:0000305|PubMed:26631749,
CC ECO:0000305|PubMed:28066457}.
CC -!- DISRUPTION PHENOTYPE: Several spikelet and inflorescence developmental
CC defects, including altered florets palea and lemma structures, abnormal
CC numbers, size, appearance, and identities of floral organs, reduced
CC primary branches number, and longer and fewer grains associated with a
CC loss of grain shattering. Crook-neck-like rachilla between the sterile
CC lemmas and the rudimentary glumes in place of the spikelet abscission
CC zone (AZ). {ECO:0000269|PubMed:22408071}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEE61805.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL606635; CAE01667.2; -; Genomic_DNA.
DR EMBL; AP014960; BAS91337.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE61805.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_015636848.1; XM_015781362.1.
DR RefSeq; XP_015636849.1; XM_015781363.1.
DR AlphaFoldDB; A0A0N7KJT8; -.
DR SMR; A0A0N7KJT8; -.
DR STRING; 4530.OS04T0649100-01; -.
DR PaxDb; A0A0N7KJT8; -.
DR EnsemblPlants; Os04t0649100-03; Os04t0649100-03; Os04g0649100.
DR GeneID; 9269072; -.
DR Gramene; Os04t0649100-03; Os04t0649100-03; Os04g0649100.
DR KEGG; osa:9269072; -.
DR OrthoDB; 1171664at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; A0A0N7KJT8; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0060860; P:regulation of floral organ abscission; IMP:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0080050; P:regulation of seed development; IMP:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEP:UniProtKB.
DR GO; GO:0097548; P:seed abscission; IMP:UniProtKB.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 2.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 2.
DR SMART; SM00380; AP2; 2.
DR SUPFAM; SSF54171; SSF54171; 2.
DR PROSITE; PS51032; AP2_ERF; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..460
FT /note="APETALA2-like protein 5"
FT /id="PRO_0000445995"
FT DNA_BIND 111..167
FT /note="AP2/ERF 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT DNA_BIND 203..260
FT /note="AP2/ERF 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 87..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 99..108
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 283..287
FT /note="EAR"
FT /evidence="ECO:0000250|UniProtKB:P47927"
FT CONFLICT 272
FT /note="Missing (in Ref. 1; CAE01667)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 460 AA; 48500 MW; 3EDE49A203C8E4CC CRC64;
MWDLNDSPAA EAAPPPLSPS ADDSGASSSS AAAVVEIPDD ADDDSAAVVV VTRQFFPPAV
PGGGGDPAPG NARAGWLRLA GAAPPVAATG PAASAAVSKK SRRGPRSRSS QYRGVTFYRR
TGRWESHIWD CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFSLED YEDDLKQMSN
LTKEEFVHVL RRQSTGFPRG SSKYRGVTLH KCGRWEARMG QFLGKKYVYL GLFDTEEEAA
RAYDRAAIKC NGKDAVTNFD PSIYAGEFEP PAAATGDAAE HNLDLSLGSS AGSKRGNVDG
GGDDEITGGG GGGAGSDQRV PMAFDLDWQT AAARSTKAKF DQNSNHPQMP PVLQVTHLPF
SPRHHHQFLS NGDPGTAGGL SLTIGAGMAG HWPPQQQQGW GNAGGMSWPH PPHPPPPPTN
AAAAATATAA AASSRFPPYI ATQASTWLQK NGFHSLTRPT
