ID   IKA1A_XENLA             Reviewed;         316 AA.
AC   A7LKB2;
DT   18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=PAK4-inhibitor inka1 {ECO:0000305};
DE   AltName: Full=Induced in neural crest by AP2-alpha protein A {ECO:0000303|PubMed:17314132};
DE            Short=IncaA {ECO:0000303|PubMed:17314132};
GN   Name=inka1-a {ECO:0000305};
GN   Synonyms=fam212a-a {ECO:0000250|UniProtKB:Q96EL1}, inca-a,
GN   incaa {ECO:0000303|PubMed:17314132};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355 {ECO:0000312|EMBL:ABS71684.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL
RP   STAGE, INDUCTION, AND INTERACTION WITH PAK5.
RX   PubMed=17314132; DOI=10.1242/dev.02813;
RA   Luo T., Xu Y., Hoffman T.L., Zhang T., Schilling T., Sargent T.D.;
RT   "Inca: a novel p21-activated kinase-associated protein required for cranial
RT   neural crest development.";
RL   Development 134:1279-1289(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Inhibitor of the serine/threonine-protein kinase pak4/pak5
CC       (By similarity). Acts by binding pak4/pak5 in a substrate-like manner,
CC       inhibiting the protein kinase activity (By similarity). Required for
CC       the proper migration of neural crest cells during embryonic
CC       development, probably by inhibiting pak4/pak5 (PubMed:17314132).
CC       {ECO:0000250|UniProtKB:Q96EL1, ECO:0000305|PubMed:17314132}.
CC   -!- SUBUNIT: Interacts with pak4/pak5. {ECO:0000269|PubMed:17314132}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96EL1}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q96EL1}.
CC   -!- DEVELOPMENTAL STAGE: Primarily detected in migrating neural crest cells
CC       and their derivatives during embryogenesis.
CC       {ECO:0000269|PubMed:17314132}.
CC   -!- INDUCTION: Expression is regulated by the transcription factor AP-2-
CC       alpha/tfap2a. {ECO:0000269|PubMed:17314132}.
CC   -!- DOMAIN: Contains 2 Inka boxes (also named iBox or inca box). The Inka
CC       boxes bind and inhibit PAK4 by binding a substrate-like manner.
CC       {ECO:0000250|UniProtKB:Q96EL1}.
CC   -!- DISRUPTION PHENOTYPE: Dramatic reduction in craniofacial cartilage
CC       formation. {ECO:0000269|PubMed:17314132}.
CC   -!- SIMILARITY: Belongs to the INKA family. {ECO:0000305}.
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DR   EMBL; BC169391; AAI69391.1; -; mRNA.
DR   EMBL; BC169393; AAI69393.1; -; mRNA.
DR   EMBL; EU030286; ABS71684.1; -; mRNA.
DR   RefSeq; NP_001099053.1; NM_001105583.1.
DR   AlphaFoldDB; A7LKB2; -.
DR   GeneID; 100126002; -.
DR   KEGG; xla:100126002; -.
DR   CTD; 100126002; -.
DR   Xenbase; XB-GENE-6254968; inka1.S.
DR   OrthoDB; 1263048at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 100126002; Expressed in neurula embryo and 16 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; ISS:UniProtKB.
DR   InterPro; IPR029267; FAM212.
DR   InterPro; IPR039201; Inka.
DR   PANTHER; PTHR28615; PTHR28615; 1.
DR   Pfam; PF15342; FAM212; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..316
FT                   /note="PAK4-inhibitor inka1"
FT                   /id="PRO_0000438817"
FT   REGION          108..130
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          182..219
FT                   /note="Inka box 1"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EL1"
FT   REGION          289..316
FT                   /note="Inka box 2"
FT                   /evidence="ECO:0000250|UniProtKB:Q96EL1"
SQ   SEQUENCE   316 AA;  35224 MW;  94FFAA8901FBA9E5 CRC64;
     MRKLHLDSLT LQLRSDLQCI KASSSSLCDQ MDCMLRVLQD LKRSSPPPSP DIEKTCAVPQ
     RRVPRRDNRI SHRTSDLSEA DSACCMDLPS DVSPGSCGQR GLEWDSGYSE VSGSSLRGEE
     DDIVEEESET RAPAVVLRRL PTPSCQRLSS GGFLNSRQGR IRPKSTSDVC LEQWRGIGLG
     SDSQDWTGCL LSQSRSRQPL ILGDNSFADL VKQWMDLPEN GDEEGRRVRD GGRWLHKPHG
     FLISLSGNVK RRLGNMSRLR HSDQEAVKRM SCPQLGCRPL SPYYHQSLSD IAEASTNLLN
     CRSRRPIICN EGAGFL